menaquinone-6 and tuberculostearic-acid

A strictly aerobic, non-motile, rod-shaped actinomycete strain, named JC2055T, was isolated from a sediment sample of getbol, the tidal flat of Korea. 16S rDNA analysis revealed that the getbol isolate belonged to the genus Nocardioides with the highest sequence similarity to Nocardioides aquiterrae GW-9T (95.5%). The major menaquinone was MK-8(H4) and the predominant cellular fatty acids were iso-16 : 0 and 17 : 1omega8c. Tuberculostearic acid was absent. The G+C content of DNA was 72 mol%. Based on the morphological, physiological, biochemical and chemotaxonomical data presented in this study, strain JC2055T can be readily differentiated from other validly named Nocardioides species. The name Nocardioides ganghwensis sp. nov. is proposed for the isolate. The type strain is JC2055T (=IMSNU 14028T=KCTC 9920T=JCM 12124T).

Topics: Actinomycetales; Aerobiosis; Bacterial Typing Techniques; Base Composition; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Geologic Sediments; Korea; Molecular Sequence Data; Movement; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Sequence Homology; Stearic Acids; Vitamin K 2; Water Microbiology

A moderately halophilic actinomycete strain, designated YIM 90010T, was isolated from a soil sample collected from a hypersaline habitat in Xinjiang Province, China, and then investigated using a polyphasic taxonomic approach. The strain produced abundant aerial mycelia and fragmented substrate mycelia on most media tested; the optimum NaCl concentration for growth was 10 % (w/v) and the optimum growth temperature and pH were 28 degrees C and 7.2, respectively. Chemotaxonomically and phylogenetically, the strain was related to members of the genus Nocardiopsis. The isolate contained chemotaxonomic markers that were diagnostic for the genus Nocardiopsis, i.e. meso-diaminopimelic acid, no diagnostic sugars, and MK-10(H6), MK-10(H8) and MK-12 as the predominant menaquinones. The major fatty acids were iso- and anteiso-branched acids combined with tuberculostearic acid (Me C(18 : 0)), straight-chain saturated fatty acids and unsaturated fatty acids. The G + C content was 73.1 mol%. Phylogenetic analysis confirmed that strain YIM 90010T was a member of the genus Nocardiopsis and most closely related to Nocardiopsis kunsanensis (97.6 % similarity) and Nocardiopsis xinjiangensis (98.1 % similarity). It can be differentiated from these species by using phenotypic characteristics, phylogenetic analysis and DNA-DNA hybridization results. On the basis of the polyphasic evidence, a novel species, Nocardiopsis salina sp. nov., is proposed. The type strain of the species is YIM 90010T (= KCTC 19003T = CCTCC AA 204009T).

Topics: Actinomycetales; Bacterial Typing Techniques; Base Composition; Carbohydrates; China; Diaminopimelic Acid; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Genes, rRNA; Growth Inhibitors; Hydrogen-Ion Concentration; Microscopy, Electron, Scanning; Molecular Sequence Data; Nucleic Acid Hybridization; Phylogeny; RNA, Bacterial; RNA, Ribosomal, 16S; Saline Solution, Hypertonic; Sequence Analysis, DNA; Soil Microbiology; Stearic Acids; Temperature; Vitamin K 2