menaquinone-6 and hopane

Strain JC207(T) was isolated from a deep (265 m) sea sediment, and appeared as dark yellow colonies on agar plates with cells staining Gram-negative. Catalase, oxidase and caseinase were positive, while chitinase, gelatinase and amylase were negative. Major (>5 %) fatty acids were iso-C15 : 0, anteiso-C15:0, iso-C17 : 1ω9c, iso-C16 : 0, iso-C15 : 0 3-OH, iso-C17 : 0 3-OH, iso-C14 : 0 and iso-C15:1G. Strain JC207(T) contained phosphatidylethanolamine as the major polar lipid, with minor amounts of five unidentified lipids. A bacterial hopane derivative, diplopterol and adenosylhopane were the major hopanoids. Genomic DNA G+C content was 47.5 mol%. 16S rRNA gene sequence comparisons indicated that strain JC207(T) represented a member of the genus Salinimicrobium within the family Flavobacteriaceae of the phylum Bacteroidetes. Strain JC207(T) had sequence similarity with Salinimicrobium terrae YIM-C338(T) (98%), Salinimicrobium xinjiangense BH206(T) (97.6 %) and other members of the genus Salinimicrobium (<96.8 %). However, strain JC207(T) showed an average of 23.6 ± 4 and 37 ± 4 relatedness (based on DNA-DNA hybridization) with Salinimicrobium terrae CGMCC 1.6308(T) ( = YIM-C338(T)) and Salinimicrobium xinjiangense KCTC 12883(T) ( = BH206(T)), respectively. Morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC207(T) as a representative of a novel species in the genus Salinimicrobium, for which the name Salinimicrobium sediminis sp. nov. is proposed. The type strain is JC207(T) ( = KCTC 32444(T) = CGMCC 1.12641(T)).

Topics: Bacterial Typing Techniques; Base Composition; DNA, Bacterial; Fatty Acids; Flavobacteriaceae; Geologic Sediments; India; Molecular Sequence Data; Nucleic Acid Hybridization; Phosphatidylethanolamines; Phylogeny; RNA, Ribosomal, 16S; Seawater; Sequence Analysis, DNA; Triterpenes; Vitamin K 2; Water Microbiology

Two strains of bacteria, JC213(T) and JC215(T), were isolated from desert soil. Colonies were red to pink and cells Gram-stain-negative. Both strains were oxidase- and catalase-positive and hydrolysed casein. In both strains, phosphatidylethanolamine was the major polar lipid, iso-C15 : 0 was the major fatty acid and the bacteriohopane derivative, BHD1, was the major hopanoid. The genomic DNA G+C contents of strains JC213(T) and JC215(T) were 52.7 and 46.3 mol%, respectively. 16S rRNA gene sequence comparisons indicated that both strains belong to the genus Pontibacter within the family Cytophagaceae and the phylum Bacteroidetes. Strain JC213(T) showed the highest sequence similarity to Pontibacter populi HLY7-15(T) (96.6 %) and with other species of the genus Pontibacter sequence similarity was less than 96 %. Strain JC215(T) exhibited highest sequence similarity with Pontibacter lucknowensis DM9(T) (95.1 %) and shared 95 % or less sequence similarity with other species of the genus Pontibacter. The sequence similarity between strains JC213(T) and JC215(T) was 95.8 %. Distinct morphological, physiological and genotypic differences from previously described taxa support JC213(T) and JC215(T) being representatives of two novel species of the genus Pontibacter, for which the names Pontibacter ruber sp. nov. and Pontibacter deserti sp. nov. are proposed and the type strains are JC213(T) ( = KCTC 32442(T) = LMG 27669(T)) and JC215(T) ( = KCTC 32443(T) = LMG 27670(T)), respectively.

Topics: Bacterial Typing Techniques; Base Composition; Cytophagaceae; Desert Climate; DNA, Bacterial; Fatty Acids; Molecular Sequence Data; Phosphatidylethanolamines; Phylogeny; Pigmentation; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Soil Microbiology; Triterpenes; Vitamin K 2