menaquinone-6 and brine

A novel Gram-stain-negative, aerobic and rod-shaped halophilic archaeon, designated HD8-45

Topics: Base Composition; China; DNA, Archaeal; Genes, Archaeal; Glycolipids; Halobacteriales; Laminaria; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Salts; Sequence Analysis, DNA; Vitamin K 2

Strain CC65(T), a novel extremely halophilic archaeon, was isolated from a brine sample of a salt lake in Iran. The novel strain was light yellow-pigmented, non-motile, pleomorphic and required at least 1.7 M NaCl and 0.02 M MgCl₂ for growth. Optimal growth was achieved at 3.5 M NaCl and 0.4 M MgCl₂. The optimum pH and temperature for growth were pH 7.5 and 40 °C, respectively, while it was able to grow over a pH and a temperature range of pH 6.5-9.0 and 30-50 °C, respectively. Analysis of 16S rRNA gene sequence revealed that strain CC65(T) clustered with the sole member of the genus Halopenitus, Halopenitus persicus DC30(T) with a sequence similarity of 98.0%. The polar lipid profile of strain CC65(T) consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. An unidentified glycolipid and two minor phospholipids were also observed. The only quinone present was MK-8(II-H₂). The DNA G+C content of strain CC65(T) was 63.8 mol%. On the basis of the biochemical and physiological characteristics, as well as DNA-DNA hybridization (44% with Halopenitus persicus IBRC 10041(T)), strain CC65(T) is classified as a novel species of the genus Halopenitus, for which the name Halopenitus malekzadehii sp. nov. is proposed. The type strain is CC65(T) ( = IBRC-M 10418(T) =KCTC 4045(T)).

Topics: Base Composition; DNA, Archaeal; Halobacteriaceae; Iran; Lakes; Magnesium Chloride; Molecular Sequence Data; Nucleic Acid Hybridization; Phospholipids; Phylogeny; RNA, Ribosomal, 16S; Salts; Sequence Analysis, DNA; Sodium Chloride; Vitamin K 2; Water Microbiology

A moderately halophilic, Gram-positive, catalase- and oxidase-positive, rod-shaped, aerobic bacterium, designated strain JSM 071077(T), was isolated from a subterranean brine sample collected from a salt mine in Hunan Province, China. Cells were motile by means of peritrichous flagella and formed ellipsoidal endospores lying in subterminal swollen sporangia. Strain JSM 071077(T) was able to grow with 2-25% (w/v) total salts (optimum, 5-10%), at pH 6.0-10.0 (optimum, pH 7.5) and 10-40 degrees C (optimum, 25-30 degrees C). meso-Diaminopimelic acid was present in the cell-wall peptidoglycan. The predominant menaquinone was MK-7, and the major cellular fatty acids were anteiso-C(15:0), anteiso-C(17:0) and iso-C(15:0). The genomic DNA G+C content was 41.8 mol%. Phylogenetic analyses based on 16S rRNA gene sequence comparisons revealed that strain JSM 071077(T) should be assigned to the genus Halobacillus, being related most closely to the type strain of Halobacillus naozhouensis (98.8% sequence similarity), and the two strains formed a distinct subline in the neighbor-joining, minimum-evolution and maximum-parsimony phylogenetic trees. The sequence similarities between the novel isolate and the type strains of other recognized Halobacillus species ranged from 97.6% (with Halobacillus alkaliphilus) to 95.2% (with Halobacillus kuroshimensis). The results of the phylogenetic analyses, combined with DNA-DNA relatedness data, phenotypic characteristics and chemotaxonomic information, support that strain JSM 071077(T) represents a new species of the genus Halobacillus, for which the name Halobacillus hunanensis sp. nov. is proposed. The type strain is JSM 071077(T) (=DSM 21184(T) = KCTC 13235(T)).

Topics: Aerobiosis; Bacillaceae; Base Composition; Catalase; Cell Wall; China; Cluster Analysis; Diaminopimelic Acid; DNA, Bacterial; DNA, Ribosomal; Fatty Acids; Flagella; Hydrogen-Ion Concentration; Locomotion; Molecular Sequence Data; Oxidoreductases; Phylogeny; RNA, Ribosomal, 16S; Salts; Sequence Analysis, DNA; Soil Microbiology; Spores, Bacterial; Vitamin K 2