menaquinone-6 and 2-hydroxyputrescine

A bacterial strain designated LYH-15T was isolated from a freshwater fish pond in Taiwan and characterized using a polyphasic taxonomy approach. Cells of LYH-15T were Gram-staining-negative, aerobic, motile by means of a single polar flagellum, poly-β-hydroxybutyrate-containing, non-spore forming, straight rods and formed light-coral-colored colonies. Growth occurred at 15-40 °C (optimum, 30 °C), at pH 5.0-9.0 (optimum, pH 7.0) and with 0-0.5 % NaCl (optimum, 0 %). Phylogenetic analyses based on 16S rRNA gene sequences showed that LYH-15T forms a distinct phyletic line within the order Burkholderiales, with less than 94 % sequence similarity to its closest relatives with validly published names. The predominant fatty acids were summed feature 3 (comprising C16 : 1ω7c and/or C16 : 1ω6c), C16 : 0 and C18 : 1ω7c. The major isoprenoid quinone was Q-8 and the DNA G+C content was 63.8 mol%. The major polar lipids were phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol and several uncharacterized lipids. The major polyamines were 2-hydroxyputrescine and putrescine. On the basis of the genotypic and phenotypic data, LYH-15T represents a novel species of a new genus in the order Burkholderiales, for which the name Piscinibacterium candidicorallinum gen. nov., sp. nov. is proposed. The type strain is LYH-15T (=BCRC 80969T=LMG 29480T=KCTC 52168T).

Topics: Animals; Aquaculture; Bacterial Typing Techniques; Base Composition; Betaproteobacteria; DNA, Bacterial; Fatty Acids; Fishes; Fresh Water; Hydroxybutyrates; Phospholipids; Phylogeny; Polyesters; Ponds; Putrescine; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Taiwan; Vitamin K 2

A bacterial strain designated CMJ-15(T) was isolated from a freshwater shrimp culture pond in Taiwan and characterized using a polyphasic taxonomic approach. Cells of strain CMJ-15(T) were facultatively anaerobic, Gram-stain-negative and motile by a single polar flagellum and formed light-yellow colonies. Growth occurred at 20-40 °C (optimum, 25-30 °C), with 0-0.7% NaCl (optimum, 0-0.2%) and at pH 5.0-9.0 (optimum, pH 6.0-7.0). Phylogenetic analyses based on 16S rRNA gene sequences showed that strain CMJ-15(T) belonged to the genus Undibacterium and its closest neighbour was Undibacterium oligocarboniphilum EM 1(T), with sequence similarity of 96.3%. The predominant cellular fatty acids were summed feature 3 (C16:1ω7c and/or C16:1ω6c), C16:0 and C18:1ω7c. The major cellular hydroxy fatty acid was C10:0 3-OH. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. The polyamine profile was composed of the major compound putrescine and moderate amounts of 2-hydroxyputrescine. The major respiratory quinone was Q-8 and the DNA G+C content was 52.2 mol%. On the basis of these phylogenetic and phenotypic data, strain CMJ-15(T) should be classified as representing a novel species, for which the name Undibacterium squillarum sp. nov. is proposed. The type strain is CMJ-15(T) ( = BCRC 80404(T) = LMG 26892(T) = KCTC 23917(T)).

Topics: Animals; Aquaculture; Bacterial Typing Techniques; Base Composition; Decapoda; DNA, Bacterial; Fatty Acids; Molecular Sequence Data; Oxalobacteraceae; Phylogeny; Pigmentation; Ponds; Putrescine; RNA, Ribosomal, 16S; Sequence Analysis, DNA; Taiwan; Vitamin K 2