melphalan and acridine-half-mustard

Three nitrogen mustard-sensitive lines of Chinese hamster ovary cells were isolated from mutagenized cultures using the procedure of Thompson et al. (1980). The lines, designated NM1, NM2 and NM3, were 2.1-, 17- and 6.8-fold more sensitive to nitrogen mustard, respectively, than their parent, wild-type, line as determined by the dose required to kill 90% of the cells, IC90. Patterns of cross-sensitivity to other DNA-damaging agents including ultraviolet light, cis-diamminedichloroplatinum, and other alkylating agents were determined for each line. Analysis of these results suggests that the phenotypes of the mutant lines are different from those lines reported previously.

Topics: Aminacrine; Animals; Cells, Cultured; Cisplatin; Cricetinae; Cross Reactions; DNA; Dose-Response Relationship, Drug; Female; Gamma Rays; In Vitro Techniques; Mechlorethamine; Melphalan; Mitomycin; Mitomycins; Mutagens; Mutation; Nitrogen Mustard Compounds