melitten and resorufin

The chemical signal communication among organelles in the cell is extremely important for life. We demonstrate here the chemical signal communication between two protoorganelles using cascade enzyme reactions in a lipid-based artificial cell. Two protoorganelles inside the artificial cell are large unilamellar vesicles containing glucose oxidase (GOx-LUVs) and a vesicle containing horseradish peroxidase (HRP) and Amplex red, respectively. The glucose molecules outside the artificial cell penetrate the lipid bilayer through mellitin pores and enter into one protoroganelle (GOx-LUV) to produce H

Topics: Armoracia; Artificial Cells; Fluorescent Dyes; Glucose; Glucose Oxidase; Horseradish Peroxidase; Hydrogen Peroxide; Lipid Bilayers; Melitten; Oxazines; Oxidation-Reduction; Unilamellar Liposomes