medrogestone and methoxyamine

A routine method for the quantitation of plasma progesterone, using only 1 ml of sample, has been developed based on gas chromatography-nitrogen detection of the O-methyloxime derivative. Simple solvent extraction of progesterone from plasma using cyclohexane as solvent and medrogestone as internal standard allows measurement at the 3 nmol/l level, while the coefficient of variation at 16, 64, and 127 nmol/l progesterone is 14, 7.5, and 6.5% respectively. Comparison with a radioimmunoassay gave a linear regression equation of Y = 0.84x + 1.4, Syx = 7.8, r = 0.9255 (n = 55).

Topics: Chromatography, Gas; Humans; Hydroxylamines; Medrogestone; Progesterone; Radioimmunoassay