masticadienolic-acid and masticadienonic-acid

Hexane extract from the bark of Amphipterygium adstringens, as well as its principal constituents, masticadienonic acid and 3alpha-hydroxymasticadienolic acid, inhibited the growth of five human cancer cell lines. Derivatives of, namely 24,25 S-dihydromasticadienonic acid and masticadienolic acid, were also evaluated. The results showed that both and had greater activity than on colon cancer cell lines. The effects of on the production of nitric oxide (NO) from both resting and lipopolysaccharide-activated macrophages were determined. It was found that and caused an increase in NO release from resting macrophages; in lipopolysaccharide-activated macrophages, only and caused an increase in NO production.

Topics: Anacardiaceae; Animals; Antineoplastic Agents, Phytogenic; Breast Neoplasms; Cell Death; Cell Line, Tumor; Central Nervous System Neoplasms; Colonic Neoplasms; Drug Screening Assays, Antitumor; Female; Hexanes; Humans; Leukemia; Lipopolysaccharides; Macrophages, Peritoneal; Male; Medicine, Traditional; Mexico; Mice; Nitric Oxide; Plant Extracts; Prostatic Neoplasms; Rhodamines; Triterpenes; Ursolic Acid

From the galls of Pistacia terebinthus we obtained an extract that proved to be effective against chronic and acute inflammation. Now we report on the isolation and identification of three triterpenes: two tirucallane-type lanostanoids and one oleanane, which we have identified as masticadienonic acid (1), masticadienolic acid (2), and morolic acid (3), respectively. All of them showed effectiveness on the mouse ear inflammation induced by repeated applications of 12-O-tetradecanoylphorbol 13-acetate and on the phospholipase A2-induced foot paw edema. The pharmacological activity of the compounds was ratified by a histological study of the ear samples. In addition, they inhibited leukotriene B4 production in rat polymorphonuclear leukocytes stimulated with calcium ionophore A 23187.

Topics: Animals; Anti-Inflammatory Agents; Calcimycin; Cyproheptadine; Dexamethasone; Dose-Response Relationship, Drug; Hindlimb; Inflammation; Ionophores; Leukotriene B4; Magnetic Resonance Spectroscopy; Mice; Neutrophils; Phospholipases A; Phospholipases A2; Pistacia; Plant Extracts; Tetradecanoylphorbol Acetate; Triterpenes