manoalide and edelfosine

manoalide has been researched along with edelfosine* in 1 studies

Other Studies

1 other study(ies) available for manoalide and edelfosine

ArticleYear
Light activation of phospholipase A2 in the photoreceptor of the crayfish (Procambarus clarkii).
    Acta neurobiologiae experimentalis, 2000, Volume: 60, Issue:1

    Retinal lipids of crayfish, kept at 4 degrees C under continuous darkness for 3 weeks, consisted mainly of phosphatidylcholine (PC) and phosphatidylethanolamine (PE); sphingomyelin (SM), phosphatidylinositol (PI) and phosphatidylserine (PS) were minor contributors. PI, involved in the phototransduction cascade, never reached greater concentrations than 7% of the total. High concentrations of polyunsaturated fatty acids (PUFA) such as 20:4n-6, 20:5n-3 and 22:6n-3 (DHA, docosahexaenoic acid) were present in PC, PE and PS, but scarce in SM and PI. In retinae of crayfish kept at 4 degrees C in darkness for 3 weeks and then exposed to white light (6 h; ca. 4,500 lx), SM and PS remained seemingly unaffected. PC, however, significantly decreased within 10 min to 65% of the initial value and 50% at 180 min. To study the reduction of PC, lipids of retinae suspended in physiological solution with/without phospholipase C (PLC) and phospholipase A2 (PLA2) inhibitors such as DMDA (= DEDA), manoalide, ET-18-OCH3, and U-73122 were measured. Only free fatty acids (FFA) of retinae with inhibitors of PLA2 like DMDA and manoalide decreased. Retinae irradiated by white light for 3 h displayed a significant reduction of PC, compared with those that had remained in continuous darkness. However, the PC of retinae with PLA2-inhibitors was not decreased by light. Our results provide evidence that not only photoreceptor cell PLC, but also PLA2 is activated by light.

    Topics: Animals; Astacoidea; Cell Membrane; Dark Adaptation; Enzyme Activation; Estrenes; Fatty Acids, Nonesterified; Fatty Acids, Unsaturated; Phosphatidylcholines; Phosphodiesterase Inhibitors; Phospholipases A; Phospholipases A2; Phospholipid Ethers; Photic Stimulation; Photoreceptor Cells, Invertebrate; Pyrrolidinones; Terpenes; Vision, Ocular

2000