mannich-bases and 2-4-6-trihydroxyacetophenone
mannich-bases has been researched along with 2-4-6-trihydroxyacetophenone* in 1 studies
Other Studies
1 other study(ies) available for mannich-bases and 2-4-6-trihydroxyacetophenone
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Molecular docking study, synthesis and biological evaluation of Mannich bases as Hsp90 inhibitors.
The ubiquitously expressed heat shock protein 90 is an encouraging target for the development of novel anticancer agents. In a program directed towards uncovering novel chemical scaffolds against Hsp90, we performed molecular docking studies using Tripos-Sybyl drug designing software by including the required conserved water molecules. The results of the docking studies predicted Mannich bases derived from 2,4-dihydroxy acetophenone/5-chloro 2,4-dihydroxy acetophenone as potential Hsp90 inhibitors. Subsequently, a few of them were synthesized (1-6) and characterized by IR, (1)H NMR, (13)C NMR and mass spectral analysis. The synthesized Mannich compounds were evaluated for their potential to suppress Hsp90 ATPase activity by the colorimetric Malachite green assay. Subsequently, the molecules were screened for their antiproilferative effect against PC3 pancreatic carcinoma cells by adopting the 3-(4,5-dimethythiazol- 2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay method. The activity profile of the identified derivatives correlated well with their docking results. Topics: Acetophenones; Adenosine Triphosphatases; Antineoplastic Agents; Cell Line, Tumor; Cell Proliferation; Chemistry Techniques, Synthetic; Drug Design; HSP90 Heat-Shock Proteins; Humans; Mannich Bases; Molecular Docking Simulation; Protein Conformation; Water | 2015 |