maneb and nabam

Mancozeb is a fungicide of the ethylene bisdithiocarbamate (EBDC) class complexed to the metals manganese and zinc. Nabam is the sodium salt of the EBDC backbone. The purpose of this study was to determine if these EBDC compounds alter essential metal homeostasis and glutathione status in Sprague-Dawley rats. Our findings indicate EBDCs caused accumulation of copper in kidneys, but not liver. EBDC compounds also increased glutathione reductase activity in liver, but not kidneys, whereas only mancozeb increased glutathione peroxidase activity in the liver. Mancozeb and nabam increased total glutathione in liver, but only mancozeb increased total glutathione in the kidney. Neither mancozeb nor nabam altered glutathione ratio in either liver or kidney compared to control. Our data suggest that the EBDC backbone of mancozeb, and not the zinc or manganese moieties, is responsible for changes in glutathione status and alteration of essential metal homeostasis in rat liver and kidney.

Topics: Animals; Ethylenebis(dithiocarbamates); Ethylenes; Fungicides, Industrial; Glutathione; Kidney; Liver; Maneb; Manganese; Metals; Rats; Rats, Sprague-Dawley; Zinc; Zineb

A simple and rapid flow-injection method is reported for the determination of dithiocarbamate fungicides (maneb, nabam and thiram) based on chemiluminescence detection. The method involves the photodegradation of dithiocarbamate fungicides via UV light in an alkaline medium. Photoproducts are then reacted with luminol in the absence of an oxidant. Linear calibration graphs were obtained in the range 0.01 - 4.0 mg L(-1) for maneb and nabam and 0.05 - 1.0 mg L(-1) for thiram with relative standard deviations (n = 4) in the range 1.0 - 2.6%. The detection limits (S/N = 3) of maneb, nabam and thiram were 10, 8.0 and 5.0 ng mL(-1), respectively, with a sample throughput of 100 h(-1). The method was successfully applied to determine these dithiocarbamate fungicides in spiked natural water samples.

Topics: Antifungal Agents; Ethylenebis(dithiocarbamates); Flow Injection Analysis; Fresh Water; Luminescent Measurements; Maneb; Photolysis; Reproducibility of Results; Sensitivity and Specificity; Thiram

Residues of EBDC (ethylenebisdithiocarbamate) fungicides and ETU (ethylenethiourea; 2-imidazolidinethione) were monitored in beers and wines from different locations. No EBDC residues were detected in any of the samples examined. Concerning the ETU residues, the residue levels higher than the limit of method detection (0.01 ppm) were 22.6% and 7.3% in the commercial beer and wine samples respectively, but the number of samples containing more than 0.1 ppm of ETU was practically negligeable.

Topics: Beer; Chromatography, Gas; Ethylenebis(dithiocarbamates); Ethylenethiourea; Fungicides, Industrial; Gas Chromatography-Mass Spectrometry; Imidazoles; Maneb; Pesticide Residues; Thiocarbamates; Wine; Zineb

Topics: Abnormalities, Drug-Induced; Animals; Chromatophores; Digestive System Abnormalities; Ear; Ethylenebis(dithiocarbamates); Eye Abnormalities; Female; Fungicides, Industrial; Maneb; Melanins; Microscopy; Microscopy, Electron; Pregnancy; Thiocarbamates; Xenopus