maneb and dodine

The effects of the fungicides dodine, benomyl, thiabendazole, mancozeb, cupric sulfate, and copper oxychloride were examined in vitro upon germination and further development of Evlachovaea sp. and Tolypocladium cylindrosporum. Fungicidal activity depended on concentrations and varied among products, fungi and the strains tested. Depending on the fungicidal concentration, germination of conidia was induced but germlings produced neither mycelium nor new conidia. There was a good recovery of both Evlachovaea sp. and T. cylindrosporum from previously sterilized soils with fungicide-supplemented medium. Fungi were resistant to copper oxychloride up to 30 g/l, and this fungicide was found to have no utility for a selective medium. Minimal fungicide concentrations for successful isolations were 1 mg/l for benomyl, 200 mg/l for cupric sulfate, 50 mg/l for dodine, 100 mg/l for mancozeb, and 4 mg/l for thiabendazole. Thiabendazole, which is easy to obtain and can be used in low quantities, showed the greatest utility for a selective medium with these entomopathogenic fungi.

Topics: Ascomycota; Benomyl; Copper; Copper Sulfate; Fungicides, Industrial; Guanidines; Hypocreales; Maneb; Mycology; Soil Microbiology; Thiabendazole; Zineb

A new bioassay has been developed combining the simplicity of direct bioautography with the improved chromatographic resolution of 2D-TLC. Mixtures of structurally diverse antifungal agents were tested to establish the validity and utility of this method in the discovery of new natural products with activity against agriculturally important fungal pathogens.

Topics: Acrylates; Aniline Compounds; Antifungal Agents; Ascomycota; Benzimidazoles; Biological Assay; Captan; Carbamates; Chromatography, Thin Layer; Colletotrichum; Dimethyldithiocarbamate; Fungicides, Industrial; Guanidines; Maneb; Methacrylates; Nitriles; Nitrobenzenes; Oxazoles; Piperazines; Plant Diseases; Plants; Pyrimidines; Strobilurins; Thiabendazole; Thiophanate; Triazoles