malonyl-coenzyme-a and hydroxyethyl-methacrylate

malonyl-coenzyme-a has been researched along with hydroxyethyl-methacrylate* in 1 studies

Other Studies

1 other study(ies) available for malonyl-coenzyme-a and hydroxyethyl-methacrylate

Article	Year
Immobilization of fatty acid synthetase from Mycobacterium smegmatis by radiation-induced polymerization. Agricultural and biological chemistry, 1990, Volume: 54, Issue:6 Fatty acid synthetase of Type I from Mycobacterium smegmatis was immobilized by radiation-induced polymerization of 2-hydroxyethyl methacrylate (HEMA) in the presence of trimethylolpropane trimethacrylate (TMPTMA). The stability of immobilized synthetase toward low ionic strength increased in comparison with the free form, but the stabilities of immobilized preparations assessed by pH and temperature were identical to those of the free form. The apparent Km of immobilized enzyme for acetyl-CoA and malonyl-CoA were both 6 microM, essentially the same as those of the free form; acetyl-CoA, 5 microM and malonyl-CoA, 6 microM. Topics: Acetyl Coenzyme A; Chromatography, Gas; Cross-Linking Reagents; Enzyme Stability; Enzymes, Immobilized; Fatty Acid Synthases; Fluorescent Antibody Technique; Hydrogen-Ion Concentration; Malonyl Coenzyme A; Methacrylates; Mycobacterium; Polymers; Temperature	1990

Article

Year

Immobilization of fatty acid synthetase from Mycobacterium smegmatis by radiation-induced polymerization.

Agricultural and biological chemistry, 1990, Volume: 54, Issue:6

Fatty acid synthetase of Type I from Mycobacterium smegmatis was immobilized by radiation-induced polymerization of 2-hydroxyethyl methacrylate (HEMA) in the presence of trimethylolpropane trimethacrylate (TMPTMA). The stability of immobilized synthetase toward low ionic strength increased in comparison with the free form, but the stabilities of immobilized preparations assessed by pH and temperature were identical to those of the free form. The apparent Km of immobilized enzyme for acetyl-CoA and malonyl-CoA were both 6 microM, essentially the same as those of the free form; acetyl-CoA, 5 microM and malonyl-CoA, 6 microM.

Topics: Acetyl Coenzyme A; Chromatography, Gas; Cross-Linking Reagents; Enzyme Stability; Enzymes, Immobilized; Fatty Acid Synthases; Fluorescent Antibody Technique; Hydrogen-Ion Concentration; Malonyl Coenzyme A; Methacrylates; Mycobacterium; Polymers; Temperature

1990