maleimide has been researched along with cysteine in 118 studies
Timeframe | Studies, this research(%) | All Research% |
---|---|---|
pre-1990 | 2 (1.69) | 18.7374 |
1990's | 4 (3.39) | 18.2507 |
2000's | 42 (35.59) | 29.6817 |
2010's | 63 (53.39) | 24.3611 |
2020's | 7 (5.93) | 2.80 |
Authors | Studies |
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Frank, JA; Krueger, RJ; Lin, C; Sarath, G | 1 |
Deziel, MR; Mau, MM | 1 |
May, JM | 1 |
Kimura, T; Sawai, T; Yamaguchi, A | 1 |
Bös, C; Braun, V | 1 |
Asai, Y; Homma, M; Kawagishi, I; Shoji, T | 1 |
Pang, T; Shigekawa, M; Su, X; Wakabayashi, S | 1 |
Blaustein, RO; Collier, RJ; Finkelstein, A; Gordon, M; Oh, KJ; Senzel, L | 1 |
Blaustein, RO; Cole, PA; Miller, C; Williams, C | 1 |
Bashtovyy, D; Hemminga, MA; Marsh, D; Páli, T | 1 |
Casey, JR; Taylor, AM; Zhu, Q | 1 |
Blaustein, RO | 1 |
Aizawa, S; Handa, H; Hasegawa, M; Hiramoto, M; Kataoka, K; Nishi, T; Sato, I; Shimizu, N; Tanaka, H; Watanabe, H; Yamaguchi, Y | 1 |
Hong, SH; Maret, W | 1 |
Handel, S; Jose, J | 1 |
Pretzer, E; Tyagarajan, K; Wiktorowicz, JE | 1 |
Lolkema, JS; Sobczak, I | 1 |
TUPPY, H; WITTER, A | 1 |
Cao, W; Matherly, LH | 1 |
Chevallet, M; Diemer, H; Leize-Wagner, E; Luche, S; Rabilloud, T; Tastet, C; Van Dorsselaer, A | 1 |
Alisio, A; Mueckler, M | 1 |
Callaghan, R; Campbell, J; Kerr, ID; Linton, KJ; Rothnie, A; Storm, J | 1 |
Jin, DY; Loiselle, DR; Pope, RM; Stafford, DW; Straight, DL; Tie, JK | 1 |
Bauer, PJ; Krause, E | 1 |
Guillemette, JG; Lang, S; Palmer, M; Spratt, DE | 1 |
Coulton, JW; Eisenhauer, HA; Pawelek, PD; Shames, S | 1 |
Boldogh, IR; McKane, M; Pon, LA; Ramcharan, S; Rubenstein, PA; Wen, KK | 1 |
Brown, MH; O'Rourke, BA; Skurray, RA; Xu, Z | 1 |
Eggleston, I; Paterson, M; Pittman, H; Torrance, L; Toth, R; Ziegler, A | 1 |
Denardo, GL; Denardo, SJ; Natarajan, A; Shi, XB; Xiong, CY | 1 |
Mark, S; Mizrachi, R; Schuldiner, S; Soskine, M; Tayer, N | 1 |
Annamalai, R; Ferreira, LC; Jiang, X; Jin, B; Kaserer, W; Klebba, PE; Ma, L; Massis, LM; Maymani, H; Newton, SM; Scott, DC; Xiao, Q | 1 |
Conrads, TP; Hardwick, JP; Hood, BL; Kim, BJ; Moon, KH; Song, BJ; Veenstra, TD | 1 |
Bokma, E; Hughes, C; Koronakis, E; Koronakis, V; Lobedanz, S; Symmons, MF | 1 |
Deutsch, C; Kobertz, WR; Lu, J | 1 |
Kamo, N; Kikukawa, T; Kouyama, T; Kurata, Y; Miyauchi, S; Nara, T | 1 |
Chen, Y; Holmes, AE; Nakanishi, K; Parr, T | 1 |
Ahlgren, S; Baastrup, B; Fant, G; Feldwisch, J; Orlova, A; Rosik, D; Sandström, M; Sjöberg, A; Tolmachev, V; Widmark, O | 1 |
Bhakta, S; Eigenbrot, C; Ervin, KE; Junutula, JR; Lowman, HB; Raab, H; Scheller, RH; Vandlen, R | 1 |
Inouye, S; Sato, J | 2 |
Herrero, RM; Leuba, G; Riederer, BM; Riederer, IM | 1 |
Fang, J; Iwasa, KH; Marriott, G; Sakata, T | 1 |
Janshoff, A; Pietuch, A; Schuy, S; Treutlein, B | 1 |
Malito, E; Manolopoulou, M; Ralat, LA; Tang, WJ; Tsay, JL; Wadlington, NL | 1 |
Hosaka, M; Kosaka, K; Saitoh, S; Satoh, T | 1 |
Beuttler, J; Frejd, FY; Kontermann, RE; Müller, D; Rothdiener, M | 1 |
Bays, E; Chang, CW; Maynard, HD; Tao, L | 1 |
Baker, JR; Nathani, RI; Smith, ME; Tedaldi, LM | 1 |
Baio, JE; Baugh, L; Castner, DG; Gamble, LJ; Nguyen, PC; Stayton, PS; Weidner, T | 1 |
Campos-Reales Pineda, NB; Caron, K; Castonguay, R; Guy, J; Jacquier, V; Keillor, JW; Michnick, SW | 1 |
Kang, ST; Yeh, CK | 1 |
Hu, G; Johnson, EF; Kemper, B | 1 |
Chen, Z; Han, X; Mello, CM; Slutsky, M; Soblosky, L | 1 |
Kurosky, A; Perez-Polo, JR; Rea, H; Savidge, TC; Soman, K; Stafford, S; Urvil, P; Wiktorowicz, JE | 1 |
Lemke, EA | 1 |
Hubálek, F; Jensen, KJ; Kjeldsen, TB; Pedersen, TA; Ribel, U; Vinther, TN | 1 |
Arion, VB; Dyson, PJ; Groessl, M; Hanif, M; Hartinger, CG; Jakupec, MA; Keppler, BK; Legin, A; Nazarov, AA; Tsybin, YO | 1 |
Kemper, B; Szczesna-Skorupa, E | 1 |
Kalle, AM; Kand, D; Talukdar, P; Varma, SJ | 1 |
Auclair, K; Cosa, G; Huang, Y; Karam, P; Ménard, A | 1 |
Fang, H; Guo, P; Haque, F; Lunn, J; Smithrud, D | 1 |
Kim, HS; Nikaido, H | 1 |
Cuccuru, MA; Dessì, D; Fiori, PL; Rappelli, P | 1 |
Barrantes-Freer, A; Bhargava, Y; Dutertre, S; Grutter, T; Kaczmarek-Hájek, K; Lörinczi, É; Marino, SF; Nicke, A; Rettinger, J; Taly, A | 1 |
Hinrichs, K; Hovestaedt, M; Rappich, J; Reut, J; Sun, G; Syritski, V; Tretjakov, A; Volkmer, R; Zhang, X | 1 |
Ball, ZT; Kundu, R | 1 |
Elduque, X; Grandas, A; Pedroso, E | 1 |
Hu, XK; Li, J; Lipson, RH | 1 |
Kaji, T; Kumagai, Y; Shinkai, Y; Toyama, T | 1 |
Dong, H; Gao, X; Guo, L; Hu, X; Qian, X; Tian, H; Tong, Y; Yao, W | 1 |
Chen, Z; Liu, Y; Marsh, EN; Ogorzalek, TL; Schroeder, MM; Yang, P | 1 |
Absalom, NL; Ambrus, JI; Balle, T; Chebib, M; Harpsøe, K; Karim, N; Lewis, TM; McLeod, MD; Qudah, T; Quek, G; von Arenstorff, I | 1 |
Chen, G; Lam, S; Murray, BW; Santoso, B | 1 |
Asano, S; Barbas, CF; Toda, N | 1 |
Brooks, CL; Chen, Z; Han, X; He, N; Wang, Z | 1 |
Liang, S; Liu, W; Liu, Y | 1 |
Akiyama, K; Endo, S; Harima, K; Kinbara, K; Muraoka, T; Noji, H; Tabata, KV; Takei, T; Tsumoto, K; Ui, M | 1 |
Hara, S; Hisabori, T; Ohuchi, Y; Tatenaka, Y | 1 |
Murray, BW; Santoso, B | 1 |
Baniulis, D; Haimi, P; Sikorskaite-Gudziuniene, S | 1 |
Bao, RM; Cheng, YZ; Tang, JB; Yang, HM | 1 |
Knipp, G; Kulczar, C; Lavan, M; Mehtala, JG; Wei, A | 1 |
Honma, E; Masuda, K; Muramoto, T; Nagatomo, K; Shinmi, D; Shiraishi, Y; Yamasaki, M | 1 |
Behnke, T; Falkenhagen, J; Hamers-Allin, C; Klein-Hartwig, K; Moser, M; Resch-Genger, U | 1 |
Bertrand, HC; Demay-Drouhard, P; Policar, C; Tabares, LC; Un, S; Vincent Ching, HY | 1 |
Anderson, GP; Shriver-Lake, LC; Taitt, CR | 1 |
Chung, J; Kim, H; Kim, S; Shin, JW; Yoon, A | 1 |
Gunnoo, SB; Madder, A | 1 |
Li, H; Li, X; Liu, G; Lou, K; Qian, R; Tang, Y; Tong, H; Wang, W; Zheng, Y; Zhou, L | 1 |
Barletta, F; Clark, T; Dirico, K; Falahaptisheh, H; Gerber, HP; Graziani, EI; Green, M; Han, X; He, H; Koehn, F; Loganzo, F; Lucas, J; Ma, D; Musto, S; O'Donnell, CJ; Puthenveetil, S; Rago, B; Sapra, P; Subramanyam, C; Teske, J; Tumey, LN; Veneziale, R | 1 |
Prabhune, M; Rehfeldt, F; Schmidt, CF; von Roden, K | 1 |
Chen, Z; Fan, X; Qian, J; Sun, Q; Yao, Y; Zhang, W | 1 |
Bublil, EM; Carrillo, RS; Kraus, JP; Majtan, T; Park, I | 1 |
Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T | 1 |
Hang, H; Percher, A; Thinon, E | 1 |
Mindell, JA; Mulligan, C | 1 |
Bridgford, JL; Chen, MZ; Hatters, DM; Hong, Y; Moily, NS; Pouladi, MA; Radwan, M; Reid, GE; Smith, TA; Song, Z; Tang, BZ; Tilley, L; Wood, RJ; Xu, X | 1 |
Cosino, E; Erickson, HK; Fourie-O'Donohue, A; He, J; Kamath, A; Kozak, KR; Lazar, GA; Lee, G; Leipold, D; Li, G; Ohri, R; Phillips, GL; Vollmar, BS; Wei, B; Xu, K; Yee, S; Yu, SF; Zhou, J | 1 |
Chang, J; Duan, W; Hou, T; Li, F; Li, H | 1 |
Bhakta, J; Bhakta, S; Bos, AB; Cook, R; Dela Cruz-Chuh, J; Erickson, H; Farahi, F; Fourie-O'Donohue, A; Junutula, JR; Kozak, KR; Liu, Y; Ng, C; Ohri, R; Pillow, TH; Polakis, P; Raab, H; Tsai, SP; Vandlen, R; Wei, B; Wong, AW | 1 |
Cistrone, PA; Dawson, PE; Deniz, AA; Lee, TC; Moran, CR | 1 |
Chen, S; Dong, L; Li, C; Locuson, C; Qian, MG | 1 |
Geiger, F; Wege, C | 1 |
Chen, Y; Hutchinson, M; Ji, J; Persson, J; Wang, J; Zheng, K; Zheng, L | 1 |
Akkapeddi, P; Bernardes, GJL; Bernardim, B; Burtoloso, ACB; Compañón, I; Corzana, F; Ferhati, X; Guerreiro, A; Jiménez-Osés, G; Matos, MJ | 1 |
Cardoso, MC; Glanz, M; Hackenberger, CPR; Helma, J; Kasper, MA; Klenk, S; Krause, E; Leonhardt, H; Penkert, M; Sauer, T; Schumacher, D; Stengl, A | 1 |
Ford, A; Ruan, X; Tavares-Carreón, F; Valvano, MA | 1 |
Brun, YV; Dalia, AB; Dalia, TN; Ellison, CK | 1 |
Cook, KM | 1 |
Honjo, T; Matsuura, K | 1 |
Krzyscik, MA; Nawrocka, D; Opaliński, Ł; Otlewski, J; Zakrzewska, M | 1 |
Brik, A; Glickman, MH; Mann, G; Satish, G; Sulkshane, P; Vamisetti, GB | 1 |
Fan, J; Hu, L; Li, H; Song, Y; Sun, Y; Zhang, R; Zheng, T | 1 |
Gober, IN; Riemen, AJ; Villain, M | 1 |
Chabenne, J; DiMarchi, RD; Finan, B; He, R; Mayer, JP; Mowery, SA | 1 |
Fan, H; Guan, L; Jia, Y; Sun, H; Sun, J; Tian, J; Wang, L; Xie, C; Xue, L; Yu, D | 1 |
Gober, IN; Sharan, R; Villain, M | 1 |
2 review(s) available for maleimide and cysteine
Article | Year |
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Maleimide-based method for elaboration of cysteine-containing peptide phage libraries.
Topics: Animals; Cysteine; ErbB Receptors; Humans; Maleimides; Peptide Library | 2015 |
Chemical Protein Modification through Cysteine.
Topics: Cysteine; Maleimides; Protein Processing, Post-Translational; Proteins | 2016 |
116 other study(ies) available for maleimide and cysteine
Article | Year |
---|---|
Bivalent ACTH antagonists: influence of peptide and spacer components on potency enhancement.
Topics: Adrenal Cortex; Adrenocorticotropic Hormone; Animals; Binding Sites; Corticosterone; Cysteine; Male; Maleimides; Peptide Fragments; Rats | 1991 |
Biotin-conjugated reagents as site-specific probes of membrane protein structure: application to the study of the human erythrocyte hexose transporter.
Topics: Biotin; Carrier Proteins; Cysteine; Erythrocyte Membrane; Galactose Oxidase; Hexoses; Humans; Hydrazines; Hydrolysis; Maleimides; Membrane Proteins; Serine Endopeptidases; Trypsin | 1990 |
Reaction of an exofacial sulfhydryl group on the erythrocyte hexose carrier with an impermeant maleimide. Relevance to the mechanism of hexose transport.
Topics: 3-O-Methylglucose; Affinity Labels; Binding, Competitive; Biological Transport; Cysteine; Cytochalasin B; Dithiothreitol; Drug Synergism; Erythrocytes; Humans; Maleimides; Maltose; Methylglucosides; Monosaccharide Transport Proteins; Phloretin; Photochemistry; Protein Conformation; Sulfhydryl Compounds | 1988 |
Remote conformational effects of the Gly-62 --> Leu mutation of the Tn10-encoded metal-tetracycline/H+ antiporter of Escherichia coli and its second-site suppressor mutation.
Topics: Amino Acid Sequence; Bacterial Proteins; Base Sequence; Cysteine; Escherichia coli; Glycine; Leucine; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Structure, Secondary; Restriction Mapping | 1997 |
Specific in vivo thiol-labeling of the FhuA outer membrane ferrichrome transport protein of Escherichia coli K-12: evidence for a disulfide bridge in the predicted gating loop.
Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Disulfides; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Hot Temperature; Ligands; Maleimides; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Receptors, Virus; Sulfhydryl Reagents | 1997 |
Cysteine-scanning mutagenesis of the periplasmic loop regions of PomA, a putative channel component of the sodium-driven flagellar motor in Vibrio alginolyticus.
Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Dithiothreitol; Electroporation; Flagella; Maleimides; Molecular Sequence Data; Mutagenesis; Protein Structure, Secondary; Sodium; Vibrio | 2000 |
A novel topology model of the human Na(+)/H(+) exchanger isoform 1.
Topics: Amino Acid Sequence; Biological Transport; Biotinylation; Cell Membrane Permeability; Cysteine; Humans; Maleimides; Mesylates; Models, Molecular; Molecular Sequence Data; Mutation; Protein Isoforms; Protein Structure, Tertiary; Sodium-Hydrogen Exchangers; Sulfhydryl Reagents; Surface Properties | 2000 |
Topography of diphtheria Toxin's T domain in the open channel state.
Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cell Membrane; Cysteine; Diphtheria Toxin; Humans; Ion Channel Gating; Ion Channels; Lipid Bilayers; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Streptavidin | 2000 |
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
Topics: Amino Acid Substitution; Animals; Binding Sites; Calibration; Cysteine; Drosophila melanogaster; Drosophila Proteins; Electric Conductivity; Ion Channel Gating; Ligands; Maleimides; Molecular Weight; Oocytes; Point Mutation; Potassium Channel Blockers; Potassium Channels; Protein Conformation; Quaternary Ammonium Compounds; Shaker Superfamily of Potassium Channels; Solvents; Tetraethylammonium; Xenopus laevis | 2000 |
Constrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer.
Topics: Amino Acid Substitution; Bacteriophage M13; Capsid; Capsid Proteins; Cysteine; Electron Spin Resonance Spectroscopy; Lipid Bilayers; Maleimides; Membrane Proteins; Models, Molecular; Mutation; Nuclear Magnetic Resonance, Biomolecular; Phospholipids; Protein Structure, Quaternary; Protein Structure, Tertiary; Spin Labels | 2001 |
Cysteine-directed cross-linking localizes regions of the human erythrocyte anion-exchange protein (AE1) relative to the dimeric interface.
Topics: Amino Acid Sequence; Anion Exchange Protein 1, Erythrocyte; Biotin; Cell Line; Cross-Linking Reagents; Cysteine; Dimerization; Humans; Maleimides; Molecular Sequence Data; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Transfection | 2001 |
Kinetics of tethering quaternary ammonium compounds to K(+) channels.
Topics: Animals; Cysteine; Kinetics; Maleimides; Oocytes; Potassium Channel Blockers; Potassium Channels; Quaternary Ammonium Compounds; Shaker Superfamily of Potassium Channels; Tetraethylammonium; Xenopus | 2002 |
Spatial redox regulation of a critical cysteine residue of NF-kappa B in vivo.
Topics: Cysteine; DNA; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation; Humans; Jurkat Cells; Maleimides; Microscopy, Fluorescence; NF-kappa B; NF-kappa B p50 Subunit; Oxidation-Reduction; Protein Binding; Recombinant Proteins; Sulfhydryl Compounds; Time Factors; Transcription Factor RelA; Transcription, Genetic | 2002 |
A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein.
Topics: Cadmium; Circular Dichroism; Cysteine; DNA, Complementary; Escherichia coli; Fluorescence Resonance Energy Transfer; Humans; Maleimides; Metallothionein; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Isoforms; Protein Structure, Tertiary; Proteins; Recombinant Fusion Proteins; Recombinant Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds; Time Factors; Ultraviolet Rays; Zinc | 2003 |
Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry.
Topics: Adrenodoxin; Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins; Base Sequence; Biotin; Cattle; Cysteine; Escherichia coli; Escherichia coli Proteins; Flow Cytometry; Fluorescein; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Transport; Recombinant Fusion Proteins; Staining and Labeling | 2003 |
Thiol-reactive dyes for fluorescence labeling of proteomic samples.
Topics: Animals; Cysteine; Fluorescent Dyes; Humans; Iodoacetamide; Isoelectric Point; Maleimides; Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds | 2003 |
Accessibility of cysteine residues in a cytoplasmic loop of CitS of Klebsiella pneumoniae is controlled by the catalytic state of the transporter.
Topics: Bacterial Proteins; Binding Sites; Carbon Isotopes; Carrier Proteins; Catalysis; Cell Membrane; Cell Membrane Permeability; Citric Acid; Cysteine; Cytoplasm; Ethylmaleimide; Klebsiella pneumoniae; Maleimides; Mesylates; Proton-Motive Force; Sodium; Transport Vesicles | 2003 |
N-(4-Dimethylamino-3,5-dinitrophenyl)maleimide: a coloured sulfhydryl reagent. Isolation and investigation of cysteine-containing peptides from human and bovine serum albumin.
Topics: Albumins; Cysteine; Humans; Maleates; Maleimides; Peptides; Serum Albumin; Serum Albumin, Bovine; Sulfhydryl Compounds; Sulfhydryl Reagents | 1960 |
Analysis of the membrane topology for transmembrane domains 7-12 of the human reduced folate carrier by scanning cysteine accessibility methods.
Topics: Animals; Biological Transport; Biotinylation; CHO Cells; Cricetinae; Cysteine; Humans; Maleimides; Membrane Transport Proteins; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Reduced Folate Carrier Protein; Sulfhydryl Reagents | 2004 |
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis.
Topics: Alkylation; Animals; Cattle; Cysteine; Cystine; Disulfides; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Gels; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Maleimides; Mass Spectrometry; Mitochondria; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Temperature; Time Factors; Water | 2004 |
Relative proximity and orientation of helices 4 and 8 of the GLUT1 glucose transporter.
Topics: Animals; Binding Sites; Cross-Linking Reagents; Cysteine; Cytoplasm; DNA, Complementary; Factor Xa; Glucose Transporter Type 1; Glycosylation; Humans; Immunoblotting; Maleimides; Models, Molecular; Monosaccharide Transport Proteins; Mutation; Oocytes; Protein Conformation; Protein Structure, Secondary; Transcription, Genetic; Xenopus laevis | 2004 |
The topography of transmembrane segment six is altered during the catalytic cycle of P-glycoprotein.
Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Boron Compounds; Catalysis; Cell Line; Cell Membrane; Codon; Coumarins; Cysteine; Electrophoresis, Polyacrylamide Gel; Hydrolysis; Insecta; Kinetics; Maleimides; Models, Chemical; Models, Molecular; Protein Conformation; Protein Isoforms; Protein Structure, Tertiary; Recombinant Proteins; Sequence Analysis, DNA | 2004 |
Chemical modification of cysteine residues is a misleading indicator of their status as active site residues in the vitamin K-dependent gamma-glutamyl carboxylation reaction.
Topics: Amino Acid Sequence; Binding Sites; Carbon-Carbon Ligases; Cysteine; Enzyme Inhibitors; Maleimides; Molecular Sequence Data; Molecular Structure; Mutagenesis, Site-Directed; Peptide Fragments; Point Mutation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stilbenes; Structure-Activity Relationship; Sulfates; Sulfhydryl Reagents; Sulfonic Acids; Trypsin; Vitamin K | 2004 |
Accessibility of cysteines in the native bovine rod cGMP-gated channel.
Topics: Amino Acid Sequence; Animals; Cattle; Conserved Sequence; Cyclic GMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Ethylmaleimide; Eye Proteins; Ion Channels; Maleimides; Molecular Sequence Data; Polyethylene Glycols; Protein Subunits; Rod Cell Outer Segment; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | 2005 |
Dual-targeted labeling of proteins using cysteine and selenomethionine residues.
Topics: Bacterial Proteins; Calmodulin; Cysteine; Energy Transfer; Escherichia coli; Fluorescence; Fluorescent Dyes; Hemolysin Proteins; Iodoacetamide; Maleimides; Mutagenesis, Site-Directed; Naphthalenesulfonates; Proteins; Selenomethionine | 2005 |
Siderophore transport through Escherichia coli outer membrane receptor FhuA with disulfide-tethered cork and barrel domains.
Topics: Bacterial Outer Membrane Proteins; Biological Transport; Blotting, Western; Cross-Linking Reagents; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Biological; Models, Molecular; Mutation; Oxygen; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Virus; Siderophores; Sulfhydryl Compounds | 2005 |
A mammalian actin substitution in yeast actin (H372R) causes a suppressible mitochondria/vacuole phenotype.
Topics: Actins; Allosteric Site; Arginine; Carbon; Crystallography, X-Ray; Cysteine; Cytoskeleton; Glucose; Glycerol; Green Fluorescent Proteins; Histidine; Maleimides; Microscopy, Fluorescence; Mitochondria; Models, Molecular; Muscle, Skeletal; Muscles; Mutagenesis; Mutation; Phenotype; Polymers; Protein Conformation; Protein Structure, Tertiary; Pyrenes; Saccharomyces cerevisiae; Temperature; Time Factors; Vacuoles | 2005 |
Role of transmembrane segment 10 in efflux mediated by the staphylococcal multidrug transport protein QacA.
Topics: Amino Acid Sequence; Aspartic Acid; Bacterial Proteins; Binding Sites; Biological Transport; Blotting, Western; Cell Membrane; Cysteine; Drug Resistance, Multiple; Escherichia coli; Kinetics; Maleimides; Membrane Transport Proteins; Methionine; Microscopy, Fluorescence; Molecular Sequence Data; Mutagenesis; Mutation; Phenotype; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Solvents; Staphylococcus aureus | 2006 |
Oriented immobilisation of engineered single-chain antibodies to develop biosensors for virus detection.
Topics: Amino Acid Sequence; Antibodies, Viral; Biosensing Techniques; Comovirus; Complementarity Determining Regions; Cysteine; Genetic Vectors; Gold; Humans; Immunoglobulin Fragments; Immunoglobulin Variable Region; Maleimides; Molecular Sequence Data; Peptide Library; Plasmids; Recombinant Fusion Proteins; Surface Plasmon Resonance | 2006 |
Development of tumor targeting anti-MUC-1 multimer: effects of di-scFv unpaired cysteine location on PEGylation and tumor binding.
Topics: Cell Line, Tumor; Chromatography, Affinity; Cysteine; Epithelial Cells; Glycosylation; Humans; Immunoglobulin Variable Region; Maleimides; Mucin-1; Polyethylene Glycols; Protein Engineering; Recombinant Proteins; Transfection | 2006 |
On parallel and antiparallel topology of a homodimeric multidrug transporter.
Topics: Antiporters; Cross-Linking Reagents; Cysteine; Detergents; Dimerization; Drug Resistance, Multiple; Escherichia coli Proteins; Hot Temperature; Kinetics; Maleimides; Models, Molecular; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Paraquat; Protein Binding; Protein Conformation | 2006 |
Evidence of ball-and-chain transport of ferric enterobactin through FepA.
Topics: Bacterial Outer Membrane Proteins; Carrier Proteins; Colicins; Cysteine; Dose-Response Relationship, Drug; Enterobactin; Escherichia coli; Fluorescein; Maleimides; Models, Molecular; Mutagenesis; Porins; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Cell Surface; Siderophores | 2007 |
Inactivation of oxidized and S-nitrosylated mitochondrial proteins in alcoholic fatty liver of rats.
Topics: Animals; Central Nervous System Depressants; Cysteine; Cytochrome P-450 CYP2E1; Ethanol; Fatty Liver, Alcoholic; Isoenzymes; Male; Maleimides; Mitochondria, Liver; Mitochondrial Proteins; Nitric Oxide Synthase; Nitrites; Nitrosation; Oxidation-Reduction; Oxidative Stress; Rats; Rats, Sprague-Dawley; Sequence Analysis, Protein | 2006 |
A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps.
Topics: Bacterial Outer Membrane Proteins; Cross-Linking Reagents; Cysteine; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Proteins; Genetic Variation; Lipoproteins; Maleimides; Membrane Transport Proteins; Models, Molecular; Multidrug Resistance-Associated Proteins; Periplasm; Protein Binding; Protein Conformation; Protein Structure, Secondary; Pseudomonas aeruginosa | 2007 |
Mapping the electrostatic potential within the ribosomal exit tunnel.
Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Kinetics; Maleimides; Microtubule-Associated Proteins; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Polyethylene Glycols; Protein Biosynthesis; Protein Conformation; Protein Folding; Ribosomes; Static Electricity | 2007 |
Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE.
Topics: Antiporters; Biological Transport; Biotin; Cell Membrane Permeability; Cysteine; Cytoplasm; Dimerization; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Mutation; Periplasm; Polyethylene; Proline; Protein Structure, Secondary; Threonine | 2007 |
Porphyrinmaleimides: towards thiol probes for cysteine residues in proteins.
Topics: Cysteine; Electrophoresis, Polyacrylamide Gel; Light; Maleimides; Porphyrins; Rhodopsin; Sulfhydryl Compounds; Ultraviolet Rays | 2008 |
Evaluation of maleimide derivative of DOTA for site-specific labeling of recombinant affibody molecules.
Topics: Animals; Binding Sites; Chelating Agents; Cysteine; Heterocyclic Compounds, 1-Ring; Histidine; Indium Radioisotopes; Maleimides; Mice; Mice, Inbred BALB C; Neoplasms; Peptides; Radionuclide Imaging; Receptor, ErbB-2; Recombinant Fusion Proteins; Sensitivity and Specificity; Transplantation, Heterologous | 2008 |
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Binding Sites; Biotin; Cell Line, Tumor; Chromatography, Liquid; Cysteine; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation; Genetic Engineering; Genetic Variation; Humans; Immunoglobulin Fab Fragments; Maleimides; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Sensitivity and Specificity; Staining and Labeling; Surface Properties | 2008 |
Recombinant aequorin with a reactive cysteine residue for conjugation with maleimide-activated antibody.
Topics: Aequorin; Amino Acid Sequence; Antibodies; Biosensing Techniques; Cysteine; Genetic Vectors; Luminescent Measurements; Maleimides; Molecular Sequence Data; Recombinant Proteins | 2008 |
Serial protein labeling with infrared maleimide dyes to identify cysteine modifications.
Topics: Affinity Labels; Aged; Cysteine; Fluorescent Dyes; Humans; Male; Maleimides; Nerve Tissue Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Sulfhydryl Compounds | 2008 |
Probing conformational changes of prestin with thiol-reactive optical switches.
Topics: Animals; Anion Transport Proteins; Biotin; Cell Membrane; Cysteine; Electric Capacitance; Guinea Pigs; Hair Cells, Auditory, Outer; Humans; Iodoacetamide; Maleimides; Optics and Photonics; Protein Conformation; Sulfhydryl Compounds; Transfection | 2008 |
In situ synthesis of lipopeptides as versatile receptors for the specific binding of nanoparticles and liposomes to solid-supported membranes.
Topics: Cysteine; Diffusion; Lipid Bilayers; Liposomes; Lysine; Maleimides; Micelles; Microscopy, Confocal; Models, Chemical; Nanoparticles; Nanotechnology; Peptides; Phospholipids; Static Electricity; Water | 2008 |
Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme.
Topics: Alkylation; Amino Acid Substitution; Catalytic Domain; Conserved Sequence; Crystallography, X-Ray; Cysteine; Enzyme Inhibitors; Humans; Insulysin; Kallidin; Maleimides; Models, Molecular; Oxidation-Reduction; Peptides; Protein Processing, Post-Translational; Substrate Specificity; Sulfhydryl Compounds | 2008 |
Simple ortho- and para-hydroquinones as compounds neuroprotective against oxidative stress in a manner associated with specific transcriptional activation.
Topics: Abietanes; Animals; Antioxidants; Cattle; Cell Line; Cysteine; Fluorescent Dyes; Glutathione; Humans; Hydroquinones; Maleimides; Neurons; Neuroprotective Agents; Oxidative Stress; Plant Extracts; Response Elements; Serum Albumin, Bovine; Transcriptional Activation | 2009 |
Targeting of epidermal growth factor receptor (EGFR)-expressing tumor cells with sterically stabilized affibody liposomes (SAL).
Topics: Antibodies; Biological Transport; Cell Line, Tumor; Cysteine; ErbB Receptors; Gene Expression Regulation, Neoplastic; Humans; Inhibitory Concentration 50; Liposomes; Maleimides; Mitoxantrone; Nanostructures; Neoplasms; Phosphatidylethanolamines; Polyethylene Glycols; Recombinant Fusion Proteins; Substrate Specificity | 2009 |
Synthesis of semitelechelic maleimide poly(PEGA) for protein conjugation by RAFT polymerization.
Topics: Chromatography, Gel; Cysteine; Electrophoresis, Polyacrylamide Gel; Maleimides; Molecular Weight; Muramidase; Polyethylene Glycols; Polymerization; Proteins | 2009 |
Bromomaleimides: new reagents for the selective and reversible modification of cysteine.
Topics: Bromine; Cysteine; Indicators and Reagents; Maleimides | 2009 |
Probing the orientation of surface-immobilized protein G B1 using ToF-SIMS, sum frequency generation, and NEXAFS spectroscopy.
Topics: Adsorption; Cysteine; Ethylene Glycol; Gold; Hydrogen-Ion Concentration; Maleimides; Molecular Structure; Mutation; Nerve Tissue Proteins; Particle Size; Spectrometry, Mass, Secondary Ion; Spectrum Analysis; Sulfhydryl Compounds; Surface Properties | 2010 |
De novo helical peptides as target sequences for a specific, fluorogenic protein labelling strategy.
Topics: Amino Acid Sequence; Cell Line; Circular Dichroism; Cysteine; ErbB Receptors; Fluorescent Dyes; Humans; Maleimides; Maltose-Binding Proteins; Microscopy, Confocal; Models, Chemical; Models, Molecular; Molecular Sequence Data; Molecular Structure; Peptides; Periplasmic Binding Proteins; Protein Structure, Secondary; Proteins; Proto-Oncogene Proteins c-fos; Staining and Labeling | 2010 |
A maleimide-based in-vitro model for ultrasound targeted imaging.
Topics: Cysteine; Gelatin; Maleimides; Models, Chemical; Surface Properties; Ultrasonics | 2011 |
CYP2C8 exists as a dimer in natural membranes.
Topics: Amino Acid Sequence; Aryl Hydrocarbon Hydroxylases; Binding Sites; Cell Line; Cross-Linking Reagents; Cysteine; Cytochrome P-450 CYP2C8; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Escherichia coli; Humans; Intracellular Membranes; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Multimerization; Transfection | 2010 |
Solvent effect and time-dependent behavior of C-terminus-cysteine-modified cecropin P1 chemically immobilized on a polymer surface.
Topics: Amino Acid Sequence; Buffers; Cysteine; Immobilized Proteins; Kinetics; Maleimides; Models, Molecular; Molecular Sequence Data; Peptides; Phosphates; Polystyrenes; Potassium Compounds; Protein Multimerization; Protein Structure, Quaternary; Reducing Agents; Solutions; Solvents; Surface Properties; Trifluoroethanol | 2011 |
Quantification of cysteinyl S-nitrosylation by fluorescence in unbiased proteomic studies.
Topics: Animals; Boron Compounds; Calorimetry; Cysteine; Female; Fluorescence; Hypoxia; Ischemia; Luminescence; Maleimides; Nitric Oxide; Perfusion; Phosphorylation; Proteomics; Random Allocation; Rats; Rats, Wistar | 2011 |
Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities.
Topics: Bacteriophage T4; Binding Sites; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Hydroxylamine; Ketones; Maleimides; Muramidase; Oximes; Phenylalanine; Protein Engineering; Recombinant Proteins; Staining and Labeling; Transformation, Genetic | 2011 |
Identification of anchor points for chemical modification of a small cysteine-rich protein by using a cysteine scan.
Topics: Alkylation; Animals; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Insulin; Male; Maleimides; Polyethylene Glycols; Rats; Rats, Wistar; Stereoisomerism; Tandem Mass Spectrometry | 2011 |
Maleimide-functionalised organoruthenium anticancer agents and their binding to thiol-containing biomolecules.
Topics: Acetylcysteine; Antineoplastic Agents; Binding Sites; Cell Line, Tumor; Cell Proliferation; Cysteine; Dose-Response Relationship, Drug; Glutathione; Humans; Maleimides; Models, Molecular; Molecular Structure; Organometallic Compounds; Ruthenium; Structure-Activity Relationship; Sulfhydryl Compounds | 2012 |
The signal-anchor sequence of CYP2C1 inserts into the membrane as a hairpin structure.
Topics: Amino Acid Sequence; Animals; Cell Membrane; Chlorocebus aethiops; COS Cells; Cysteine; Cytochrome P-450 CYP2E1; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; HEK293 Cells; Humans; Maleimides; Molecular Sequence Data; Polyethylene Glycols; Protein Sorting Signals; Protein Structure, Secondary | 2011 |
A chromenoquinoline-based fluorescent off-on thiol probe for bioimaging.
Topics: Benzopyrans; Cell Line, Tumor; Cell Membrane Permeability; Cysteine; Fluorescent Dyes; Humans; Maleimides; Microscopy, Fluorescence; Quantum Theory; Quinolines; Sulfhydryl Compounds | 2012 |
Site-specific fluorescent labeling and oriented immobilization of a triple mutant of CYP3A4 via C64.
Topics: Amino Acid Sequence; Binding Sites; Cysteine; Cytochrome P-450 CYP3A; Enzymes, Immobilized; Fluorescent Dyes; Humans; Maleimides; Microspheres; Models, Molecular; Molecular Sequence Data; Mutant Proteins; Mutation; Protein Conformation; Silicon Dioxide; Substrate Specificity | 2012 |
Real-time sensing and discrimination of single chemicals using the channel of phi29 DNA packaging nanomotor.
Topics: Bacillus Phages; Benzene; Biosensing Techniques; Cysteine; DNA Packaging; DNA, Viral; Ethane; Maleimides; Models, Molecular; Mutation; Nanopores; Nucleic Acid Conformation; Protein Conformation; Thymine; Time Factors; Viral Proteins | 2012 |
Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays.
Topics: Aequorin; alpha-Fetoproteins; Biotin; Cysteine; Histidine; Immunoassay; Luminescent Measurements; Maleimides; Models, Chemical; Recombinant Fusion Proteins; Sensitivity and Specificity; Streptavidin | 2012 |
Different functions of MdtB and MdtC subunits in the heterotrimeric efflux transporter MdtB(2)C complex of Escherichia coli.
Topics: Amino Acid Sequence; Biological Transport; Cloxacillin; Cysteine; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Multimerization | 2012 |
A simple, rapid and inexpensive technique to bind small peptides to polystyrene surfaces for immunoenzymatic assays.
Topics: Antibodies; Carrier Proteins; Cysteine; Enzyme-Linked Immunosorbent Assay; Maleimides; Peptides; Polystyrenes; Surface Properties | 2012 |
Involvement of the cysteine-rich head domain in activation and desensitization of the P2X1 receptor.
Topics: Animals; Cations; Cell Membrane; Crystallography, X-Ray; Cysteine; DNA, Complementary; Electrophysiology; Kinetics; Maleimides; Microscopy, Fluorescence; Mutation; Oocytes; Protein Binding; Protein Structure, Tertiary; Rats; Receptors, Purinergic P2X1; Rhodamines; Xenopus; Xenopus laevis | 2012 |
Electrochemical functionalization of gold and silicon surfaces by a maleimide group as a biosensor for immunological application.
Topics: Antibodies; Biosensing Techniques; Cysteine; Electrochemical Techniques; Electrodes; Gold; Immobilized Proteins; Maleimides; Peptides; Silicon; Spectrophotometry, Infrared; Surface Properties | 2013 |
Rhodium-catalyzed cysteine modification with diazo reagents.
Topics: Azo Compounds; Catalysis; Coordination Complexes; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Humans; Maleimides; Rhodium | 2013 |
Straightforward synthesis of cyclic and bicyclic peptides.
Topics: Cyclization; Cysteine; Maleimides; Molecular Structure; Peptides; Peptides, Cyclic | 2013 |
On-chip enrichment and analysis of peptide subsets using a maleimide-functionalized fluorous affinity biochip and nanostructure initiator mass spectrometry.
Topics: Affinity Labels; Cysteine; Fluorocarbons; Maleimides; Microarray Analysis; Nanostructures; Peptide Fragments; Serum Albumin, Bovine; Silicon; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry | 2013 |
Convenient method to assess chemical modification of protein thiols by electrophilic metals.
Topics: Adaptor Proteins, Signal Transducing; Animals; Biotin; Cysteine; Cytoskeletal Proteins; Electrophoresis, Polyacrylamide Gel; Hepatocytes; Humans; Kelch-Like ECH-Associated Protein 1; Male; Maleimides; Methylmercury Compounds; Mice; Mice, Inbred C57BL; Neuroblastoma; Sulfhydryl Compounds; Tumor Cells, Cultured | 2013 |
Characterization of a site-specific PEGylated analog of exendin-4 and determination of the PEGylation site.
Topics: Amino Acid Sequence; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Cysteine; Electrophoresis, Polyacrylamide Gel; Exenatide; Maleimides; Molecular Sequence Data; Peptide Mapping; Peptides; Polyethylene Glycols; Protein Conformation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Technology, Pharmaceutical; Venoms | 2013 |
Molecular orientation of enzymes attached to surfaces through defined chemical linkages at the solid-liquid interface.
Topics: Binding Sites; Cysteine; Enzymes, Immobilized; Ethylene Glycol; Glycoside Hydrolases; Lactococcus lactis; Maleimides; Models, Molecular; Surface Properties | 2013 |
Covalent trapping of methyllycaconitine at the α4-α4 interface of the α4β2 nicotinic acetylcholine receptor: antagonist binding site and mode of receptor inhibition revealed.
Topics: Aconitine; Animals; Binding Sites; Cysteine; Escherichia coli; Female; Ligands; Maleimides; Mutagenesis, Site-Directed; Nicotinic Antagonists; Oocytes; Protein Binding; Protein Conformation; Rats; Receptors, Nicotinic; Recombinant Proteins; Xenopus laevis | 2013 |
A simple and efficient maleimide-based approach for peptide extension with a cysteine-containing peptide phage library.
Topics: Amino Acid Sequence; Cysteine; ErbB Receptors; Humans; Maleimides; Molecular Sequence Data; Peptide Library; Peptides; Protein Binding; Recombinant Fusion Proteins | 2013 |
Rapid, stable, chemoselective labeling of thiols with Julia-Kocieński-like reagents: a serum-stable alternative to maleimide-based protein conjugation.
Topics: Click Chemistry; Cysteine; Humans; Maleimides; Models, Molecular; Oxadiazoles; Plasma; Protein Stability; Proteins; Sulfhydryl Compounds | 2013 |
Molecular structures of C- and N-terminus cysteine modified cecropin P1 chemically immobilized onto maleimide-terminated self-assembled monolayers investigated by molecular dynamics simulation.
Topics: Amino Acid Sequence; Anti-Infective Agents; Cysteine; Immobilized Proteins; Maleimides; Molecular Dynamics Simulation; Peptides; Protein Structure, Secondary; Surface Properties; Vibration; Water | 2014 |
Two-photon fluorescent probe derived from naphthalimide for cysteine detection and imaging in living cells.
Topics: Cysteine; Fluorescent Dyes; Glutathione; HeLa Cells; Homocysteine; Humans; Magnetic Resonance Spectroscopy; Maleimides; Models, Chemical; Molecular Probe Techniques; Naphthalimides; Photons; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds | 2015 |
Grafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activity.
Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Bacterial Toxins; Cysteine; Erythrocytes; Fluoresceins; Gene Expression Regulation; Hemolysin Proteins; Hemolysis; Hydrogen-Ion Concentration; Maleimides; Microscopy, Electron, Transmission; Molecular Sequence Data; Molecular Structure; Mutation; Protein Conformation; Protein Engineering; Sheep; Staphylococcus aureus | 2014 |
Direct determination of the redox status of cysteine residues in proteins in vivo.
Topics: Arabidopsis; Blotting, Western; Cysteine; DNA; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; HeLa Cells; Humans; Maleimides; Oxidation-Reduction; Oxidative Stress; Proteins; Sulfhydryl Compounds; Ultraviolet Rays | 2015 |
Application of multiplexed cysteine-labeled complex protein sample for 2D electrophoretic gel alignment.
Topics: Benzopyrans; Cysteine; Electrophoresis, Gel, Two-Dimensional; Fluorescent Dyes; Image Processing, Computer-Assisted; Indoles; Maleimides; Malus; Pilot Projects; Plant Proteins; Software | 2015 |
Site-specific covalent attachment of an engineered Z-domain onto a solid matrix: an efficient platform for 3D IgG immobilization.
Topics: Antibodies, Immobilized; Chromatography, Affinity; Chromatography, Gel; Cysteine; Immunoglobulin G; Maleimides; Peptides; Polystyrenes; Protein Engineering; Protein Structure, Tertiary; Surface Properties | 2015 |
Cys34-PEGylated Human Serum Albumin for Drug Binding and Delivery.
Topics: Cell Survival; Chemistry, Pharmaceutical; Cysteine; Drug Carriers; Humans; Maleimides; MCF-7 Cells; Models, Molecular; Nanoparticles; Paclitaxel; Permeability; Polyethylene Glycols; Protein Conformation; Serum Albumin | 2015 |
Identification of highly reactive cysteine residues at less exposed positions in the Fab constant region for site-specific conjugation.
Topics: Cell Line, Tumor; Cysteine; Escherichia coli; Humans; Immunoconjugates; Immunoglobulin Fab Fragments; Maleimides; Polyethylene Glycols; Protein Engineering; Protein Stability; Sulfhydryl Compounds | 2015 |
Quantification of PEG-maleimide ligands and coupling efficiencies on nanoparticles with Ellman's reagent.
Topics: Coloring Agents; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Ligands; Maleimides; Nanoparticles; Polyethylene Glycols; Polystyrenes | 2015 |
Nanometric distance measurements between Mn(ii)DOTA centers.
Topics: Coordination Complexes; Cysteine; Electron Spin Resonance Spectroscopy; Heterocyclic Compounds, 1-Ring; Maleimides; Manganese; Models, Molecular; Peptides; Protein Structure, Secondary; Spin Labels | 2015 |
Oriented Peptide Immobilization on Microspheres.
Topics: Amines; Antimicrobial Cationic Peptides; Carbodiimides; Cysteine; Immobilized Proteins; Maleimides; Microspheres; Polyethylene Glycols; Protein Array Analysis; Protein Structure, Secondary; Succinimides; Surface Properties | 2016 |
Chicken scFvs with an Artificial Cysteine for Site-Directed Conjugation.
Topics: Amino Acid Substitution; Animals; Chickens; Cysteine; Immunoconjugates; Immunoglobulin kappa-Chains; Maleimides; Mutagenesis, Site-Directed; Mutation; Recombinant Fusion Proteins; Single-Chain Antibodies; Sulfhydryl Compounds | 2016 |
Rational Design of an Ultrasensitive and Highly Selective Chemodosimeter by a Dual Quenching Mechanism for Cysteine Based on a Facile Michael-Transcyclization Cascade Reaction.
Topics: Click Chemistry; Cyclization; Cysteine; Electron Transport; Fluorescent Dyes; Glutathione; HeLa Cells; Homocysteine; Humans; Limit of Detection; Maleimides; Microscopy, Fluorescence; Quantum Theory; Spectrometry, Fluorescence | 2016 |
Natural Product Splicing Inhibitors: A New Class of Antibody-Drug Conjugate (ADC) Payloads.
Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biological Products; Carboxylic Acids; Cell Line, Tumor; Cell Transformation, Neoplastic; Cysteine; Humans; Immunoconjugates; Lysine; Maleimides; Mice; Pyrans; Tissue Distribution | 2016 |
Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro.
Topics: Animals; Antineoplastic Agents, Phytogenic; Cysteine; Esters; Maleimides; Microtubules; Paclitaxel; Polymers; Succinimides; Swine; Tubulin | 2016 |
Highly sensitive detection of cysteine over glutathione and homo-cysteine: New insight into the Michael addition of mercapto group to maleimide.
Topics: Animals; Biosensing Techniques; Cattle; Cell Line; Coumarins; Cysteine; Fluorescent Dyes; Glutathione; Homocysteine; Limit of Detection; Maleimides; Mice; Models, Molecular; Optical Imaging; Serum Albumin, Bovine; Spectrometry, Fluorescence; Sulfhydryl Compounds | 2017 |
Engineering and Characterization of an Enzyme Replacement Therapy for Classical Homocystinuria.
Topics: Amino Acid Sequence; Animals; Cross-Linking Reagents; Cystathionine beta-Synthase; Cysteine; Delayed-Action Preparations; Disease Models, Animal; Enzyme Replacement Therapy; Homocysteine; Homocystinuria; Humans; Maleimides; Mice; Polyethylene Glycols; Succinimides | 2017 |
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases | 2018 |
Mass-Tag Labeling Using Acyl-PEG Exchange for the Determination of Endogenous Protein S-Fatty Acylation.
Topics: Acrylates; Acylation; Cell Line; Cysteine; Fatty Acids; HEK293 Cells; Humans; Maleimides; Polyethylene Glycols; Protein Binding; Proteins | 2017 |
Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling.
Topics: Biochemistry; Biological Transport; Carrier Proteins; Cross-Linking Reagents; Cysteine; Electrophoresis, Polyacrylamide Gel; Maleimides; Mass Spectrometry; Mercury; Mesylates; Protein Conformation | 2017 |
A thiol probe for measuring unfolded protein load and proteostasis in cells.
Topics: Animals; Artemisinins; Cells; Cysteine; Fluorescent Dyes; HEK293 Cells; HeLa Cells; Humans; Huntingtin Protein; Malaria; Maleimides; Mice; Molecular Probes; Mutant Proteins; Oligopeptides; Parasites; Protein Folding; Proteome; Proteostasis; Solubility; Sulfhydryl Compounds; Tunicamycin | 2017 |
Attachment Site Cysteine Thiol pK
Topics: Benzodiazepines; Cysteine; Drug Stability; Immunoconjugates; Maleimides; Models, Molecular; Mutation; Protein Conformation; Pyrroles | 2017 |
Paper-based fluorescent sensor via aggregation induced emission fluorogen for facile and sensitive visual detection of hydrogen peroxide and glucose.
Topics: Biosensing Techniques; Blood Glucose; Cysteine; Fluorescent Dyes; Glucose Oxidase; Humans; Hydrogen Peroxide; Limit of Detection; Maleimides; Oxidation-Reduction; Reactive Oxygen Species; Stilbenes | 2018 |
High-Throughput Cysteine Scanning To Identify Stable Antibody Conjugation Sites for Maleimide- and Disulfide-Based Linkers.
Topics: Animals; Antineoplastic Agents, Immunological; Cysteine; Disulfides; Drug Stability; High-Throughput Screening Assays; Humans; Immunoconjugates; Maleimides; Models, Molecular; Mutagenesis, Site-Directed; Oligopeptides; Protein Aggregates; Protein Stability; Rats; Trastuzumab | 2018 |
Site-Specific Three-Color Labeling of α-Synuclein via Conjugation to Uniquely Reactive Cysteines during Assembly by Native Chemical Ligation.
Topics: alpha-Synuclein; Color; Cysteine; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; Maleimides; Protein Conformation | 2018 |
A Two-Step Immunocapture LC/MS/MS Assay for Plasma Stability and Payload Migration Assessment of Cysteine-Maleimide-Based Antibody Drug Conjugates.
Topics: Albumins; Animals; Antibodies, Monoclonal; Antibodies, Monoclonal, Humanized; Chromatography, Liquid; Cysteine; Humans; Immunoassay; Immunoconjugates; Maleimides; Mice; Tandem Mass Spectrometry | 2018 |
Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits.
Topics: Amino Acid Sequence; Capsid Proteins; Cysteine; Maleimides; Nanotechnology; Nicotiana; Protein Subunits; RNA, Viral; Succinimides; Sulfhydryl Compounds; Tobacco Mosaic Virus | 2018 |
Characterization of Ring-Opening Reaction of Succinimide Linkers in ADCs.
Topics: Antibodies, Monoclonal; Antineoplastic Agents; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Cysteine; Hydrolysis; Immunoconjugates; Maleimides; Succinimides; Sulfhydryl Compounds | 2019 |
Efficient and irreversible antibody-cysteine bioconjugation using carbonylacrylic reagents.
Topics: Acrylates; Analytic Sample Preparation Methods; Chromatography, Liquid; Cross-Linking Reagents; Cysteine; Humans; Immunoconjugates; Immunoglobulins; Maleimides; Single-Domain Antibodies; Tandem Mass Spectrometry | 2019 |
Cysteine-Selective Phosphonamidate Electrophiles for Modular Protein Bioconjugations.
Topics: Antineoplastic Agents, Immunological; Cysteine; Humans; Immunoconjugates; Iodoacetamide; Maleimides; Organophosphonates; Receptor, ErbB-2; Sulfhydryl Compounds; Trastuzumab | 2019 |
Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolysaccharide Biosynthesis.
Topics: Bacteria; Bacterial Proteins; Biosynthetic Pathways; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Genes, Bacterial; Lipopolysaccharides; Maleimides; Mutagenesis, Site-Directed; Polyethylene Glycols; Staining and Labeling; Sulfhydryl Compounds | 2019 |
Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules.
Topics: Biotin; Caulobacter; Cysteine; Fimbriae, Bacterial; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Molecular; Optical Imaging; Staining and Labeling; Vibrio cholerae | 2019 |
Determining the Redox Potential of a Protein Disulphide Bond.
Topics: Biotin; Cysteine; Disulfides; Maleimides; Oxidation-Reduction; Protein Domains; Protein Folding; Proteins; Sulfhydryl Compounds; Tandem Mass Spectrometry | 2019 |
Artificial Viral Capsid Dressed Up with Human Serum Albumin.
Topics: Capsid; Cross-Linking Reagents; Cysteine; Humans; Maleimides; Models, Molecular; Nanocapsules; Peptides; Serum Albumin, Human | 2019 |
Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential.
Topics: Animals; Apoptosis; Cell Line, Tumor; Cell Movement; Cysteine; Fibroblast Growth Factor 2; Heparin; Humans; Maleimides; Mice; Mitogens; Mitosis; NIH 3T3 Cells; Osteosarcoma; Polyethylene Glycols; Protein Engineering; Protein Multimerization; Receptor, Fibroblast Growth Factor, Type 1; Recombinant Proteins | 2020 |
On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins.
Topics: Catalysis; Coordination Complexes; Cycloaddition Reaction; Cysteine; Disulfides; Globins; Inteins; Maleimides; Palladium; Peptides; Proteins; Solid-Phase Synthesis Techniques; Succinimides; Thiazolidines; Thioredoxins; Ubiquitin; Ubiquitin Thiolesterase | 2020 |
Ultrasensitive and selective fluorescent sensor for cysteine and application to drug analysis and bioimaging.
Topics: Coumarins; Cysteine; Fluorescent Dyes; HeLa Cells; Humans; Maleimides; Molecular Structure; Optical Imaging | 2021 |
Sequence sensitivity and pH dependence of maleimide conjugated N-terminal cysteine peptides to thiazine rearrangement.
Topics: Cysteine; Hydrogen-Ion Concentration; Maleimides; Molecular Structure; Peptides; Thiazines | 2021 |
A Facile Procedure for One-Pot Stable Conjugation of Two Proglucagon Cysteine-Containing Peptide Analogs.
Topics: Amino Acid Sequence; Animals; Cells, Cultured; Cricetinae; Cysteine; Glucagon-Like Peptide-1 Receptor; Humans; Maleimides; Organic Chemistry Phenomena; Peptides; Polymers; Proglucagon | 2021 |
Rapid GSH detection and versatile peptide/protein labelling to track cell penetration using coumarin-based probes.
Topics: Animals; Coumarins; Cysteine; Fluorescent Dyes; Glutathione; Homocysteine; Nucleosomes; Peptides; Sulfhydryl Compounds; Zebrafish | 2023 |
Improving the stability of thiol-maleimide bioconjugates via the formation of a thiazine structure.
Topics: Cysteine; Glutathione; Maleimides; Peptides; Sulfhydryl Compounds | 2023 |