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maleimide and cysteine

maleimide has been researched along with cysteine in 118 studies

Research

Studies (118)

TimeframeStudies, this research(%)All Research%
pre-19902 (1.69)18.7374
1990's4 (3.39)18.2507
2000's42 (35.59)29.6817
2010's63 (53.39)24.3611
2020's7 (5.93)2.80

Authors

AuthorsStudies
Frank, JA; Krueger, RJ; Lin, C; Sarath, G1
Deziel, MR; Mau, MM1
May, JM1
Kimura, T; Sawai, T; Yamaguchi, A1
Bös, C; Braun, V1
Asai, Y; Homma, M; Kawagishi, I; Shoji, T1
Pang, T; Shigekawa, M; Su, X; Wakabayashi, S1
Blaustein, RO; Collier, RJ; Finkelstein, A; Gordon, M; Oh, KJ; Senzel, L1
Blaustein, RO; Cole, PA; Miller, C; Williams, C1
Bashtovyy, D; Hemminga, MA; Marsh, D; Páli, T1
Casey, JR; Taylor, AM; Zhu, Q1
Blaustein, RO1
Aizawa, S; Handa, H; Hasegawa, M; Hiramoto, M; Kataoka, K; Nishi, T; Sato, I; Shimizu, N; Tanaka, H; Watanabe, H; Yamaguchi, Y1
Hong, SH; Maret, W1
Handel, S; Jose, J1
Pretzer, E; Tyagarajan, K; Wiktorowicz, JE1
Lolkema, JS; Sobczak, I1
TUPPY, H; WITTER, A1
Cao, W; Matherly, LH1
Chevallet, M; Diemer, H; Leize-Wagner, E; Luche, S; Rabilloud, T; Tastet, C; Van Dorsselaer, A1
Alisio, A; Mueckler, M1
Callaghan, R; Campbell, J; Kerr, ID; Linton, KJ; Rothnie, A; Storm, J1
Jin, DY; Loiselle, DR; Pope, RM; Stafford, DW; Straight, DL; Tie, JK1
Bauer, PJ; Krause, E1
Guillemette, JG; Lang, S; Palmer, M; Spratt, DE1
Coulton, JW; Eisenhauer, HA; Pawelek, PD; Shames, S1
Boldogh, IR; McKane, M; Pon, LA; Ramcharan, S; Rubenstein, PA; Wen, KK1
Brown, MH; O'Rourke, BA; Skurray, RA; Xu, Z1
Eggleston, I; Paterson, M; Pittman, H; Torrance, L; Toth, R; Ziegler, A1
Denardo, GL; Denardo, SJ; Natarajan, A; Shi, XB; Xiong, CY1
Mark, S; Mizrachi, R; Schuldiner, S; Soskine, M; Tayer, N1
Annamalai, R; Ferreira, LC; Jiang, X; Jin, B; Kaserer, W; Klebba, PE; Ma, L; Massis, LM; Maymani, H; Newton, SM; Scott, DC; Xiao, Q1
Conrads, TP; Hardwick, JP; Hood, BL; Kim, BJ; Moon, KH; Song, BJ; Veenstra, TD1
Bokma, E; Hughes, C; Koronakis, E; Koronakis, V; Lobedanz, S; Symmons, MF1
Deutsch, C; Kobertz, WR; Lu, J1
Kamo, N; Kikukawa, T; Kouyama, T; Kurata, Y; Miyauchi, S; Nara, T1
Chen, Y; Holmes, AE; Nakanishi, K; Parr, T1
Ahlgren, S; Baastrup, B; Fant, G; Feldwisch, J; Orlova, A; Rosik, D; Sandström, M; Sjöberg, A; Tolmachev, V; Widmark, O1
Bhakta, S; Eigenbrot, C; Ervin, KE; Junutula, JR; Lowman, HB; Raab, H; Scheller, RH; Vandlen, R1
Inouye, S; Sato, J2
Herrero, RM; Leuba, G; Riederer, BM; Riederer, IM1
Fang, J; Iwasa, KH; Marriott, G; Sakata, T1
Janshoff, A; Pietuch, A; Schuy, S; Treutlein, B1
Malito, E; Manolopoulou, M; Ralat, LA; Tang, WJ; Tsay, JL; Wadlington, NL1
Hosaka, M; Kosaka, K; Saitoh, S; Satoh, T1
Beuttler, J; Frejd, FY; Kontermann, RE; Müller, D; Rothdiener, M1
Bays, E; Chang, CW; Maynard, HD; Tao, L1
Baker, JR; Nathani, RI; Smith, ME; Tedaldi, LM1
Baio, JE; Baugh, L; Castner, DG; Gamble, LJ; Nguyen, PC; Stayton, PS; Weidner, T1
Campos-Reales Pineda, NB; Caron, K; Castonguay, R; Guy, J; Jacquier, V; Keillor, JW; Michnick, SW1
Kang, ST; Yeh, CK1
Hu, G; Johnson, EF; Kemper, B1
Chen, Z; Han, X; Mello, CM; Slutsky, M; Soblosky, L1
Kurosky, A; Perez-Polo, JR; Rea, H; Savidge, TC; Soman, K; Stafford, S; Urvil, P; Wiktorowicz, JE1
Lemke, EA1
Hubálek, F; Jensen, KJ; Kjeldsen, TB; Pedersen, TA; Ribel, U; Vinther, TN1
Arion, VB; Dyson, PJ; Groessl, M; Hanif, M; Hartinger, CG; Jakupec, MA; Keppler, BK; Legin, A; Nazarov, AA; Tsybin, YO1
Kemper, B; Szczesna-Skorupa, E1
Kalle, AM; Kand, D; Talukdar, P; Varma, SJ1
Auclair, K; Cosa, G; Huang, Y; Karam, P; Ménard, A1
Fang, H; Guo, P; Haque, F; Lunn, J; Smithrud, D1
Kim, HS; Nikaido, H1
Cuccuru, MA; Dessì, D; Fiori, PL; Rappelli, P1
Barrantes-Freer, A; Bhargava, Y; Dutertre, S; Grutter, T; Kaczmarek-Hájek, K; Lörinczi, É; Marino, SF; Nicke, A; Rettinger, J; Taly, A1
Hinrichs, K; Hovestaedt, M; Rappich, J; Reut, J; Sun, G; Syritski, V; Tretjakov, A; Volkmer, R; Zhang, X1
Ball, ZT; Kundu, R1
Elduque, X; Grandas, A; Pedroso, E1
Hu, XK; Li, J; Lipson, RH1
Kaji, T; Kumagai, Y; Shinkai, Y; Toyama, T1
Dong, H; Gao, X; Guo, L; Hu, X; Qian, X; Tian, H; Tong, Y; Yao, W1
Chen, Z; Liu, Y; Marsh, EN; Ogorzalek, TL; Schroeder, MM; Yang, P1
Absalom, NL; Ambrus, JI; Balle, T; Chebib, M; Harpsøe, K; Karim, N; Lewis, TM; McLeod, MD; Qudah, T; Quek, G; von Arenstorff, I1
Chen, G; Lam, S; Murray, BW; Santoso, B1
Asano, S; Barbas, CF; Toda, N1
Brooks, CL; Chen, Z; Han, X; He, N; Wang, Z1
Liang, S; Liu, W; Liu, Y1
Akiyama, K; Endo, S; Harima, K; Kinbara, K; Muraoka, T; Noji, H; Tabata, KV; Takei, T; Tsumoto, K; Ui, M1
Hara, S; Hisabori, T; Ohuchi, Y; Tatenaka, Y1
Murray, BW; Santoso, B1
Baniulis, D; Haimi, P; Sikorskaite-Gudziuniene, S1
Bao, RM; Cheng, YZ; Tang, JB; Yang, HM1
Knipp, G; Kulczar, C; Lavan, M; Mehtala, JG; Wei, A1
Honma, E; Masuda, K; Muramoto, T; Nagatomo, K; Shinmi, D; Shiraishi, Y; Yamasaki, M1
Behnke, T; Falkenhagen, J; Hamers-Allin, C; Klein-Hartwig, K; Moser, M; Resch-Genger, U1
Bertrand, HC; Demay-Drouhard, P; Policar, C; Tabares, LC; Un, S; Vincent Ching, HY1
Anderson, GP; Shriver-Lake, LC; Taitt, CR1
Chung, J; Kim, H; Kim, S; Shin, JW; Yoon, A1
Gunnoo, SB; Madder, A1
Li, H; Li, X; Liu, G; Lou, K; Qian, R; Tang, Y; Tong, H; Wang, W; Zheng, Y; Zhou, L1
Barletta, F; Clark, T; Dirico, K; Falahaptisheh, H; Gerber, HP; Graziani, EI; Green, M; Han, X; He, H; Koehn, F; Loganzo, F; Lucas, J; Ma, D; Musto, S; O'Donnell, CJ; Puthenveetil, S; Rago, B; Sapra, P; Subramanyam, C; Teske, J; Tumey, LN; Veneziale, R1
Prabhune, M; Rehfeldt, F; Schmidt, CF; von Roden, K1
Chen, Z; Fan, X; Qian, J; Sun, Q; Yao, Y; Zhang, W1
Bublil, EM; Carrillo, RS; Kraus, JP; Majtan, T; Park, I1
Demizu, Y; Hattori, T; Kurihara, M; Misawa, T; Naito, M; Okitsu, K; Shoda, T1
Hang, H; Percher, A; Thinon, E1
Mindell, JA; Mulligan, C1
Bridgford, JL; Chen, MZ; Hatters, DM; Hong, Y; Moily, NS; Pouladi, MA; Radwan, M; Reid, GE; Smith, TA; Song, Z; Tang, BZ; Tilley, L; Wood, RJ; Xu, X1
Cosino, E; Erickson, HK; Fourie-O'Donohue, A; He, J; Kamath, A; Kozak, KR; Lazar, GA; Lee, G; Leipold, D; Li, G; Ohri, R; Phillips, GL; Vollmar, BS; Wei, B; Xu, K; Yee, S; Yu, SF; Zhou, J1
Chang, J; Duan, W; Hou, T; Li, F; Li, H1
Bhakta, J; Bhakta, S; Bos, AB; Cook, R; Dela Cruz-Chuh, J; Erickson, H; Farahi, F; Fourie-O'Donohue, A; Junutula, JR; Kozak, KR; Liu, Y; Ng, C; Ohri, R; Pillow, TH; Polakis, P; Raab, H; Tsai, SP; Vandlen, R; Wei, B; Wong, AW1
Cistrone, PA; Dawson, PE; Deniz, AA; Lee, TC; Moran, CR1
Chen, S; Dong, L; Li, C; Locuson, C; Qian, MG1
Geiger, F; Wege, C1
Chen, Y; Hutchinson, M; Ji, J; Persson, J; Wang, J; Zheng, K; Zheng, L1
Akkapeddi, P; Bernardes, GJL; Bernardim, B; Burtoloso, ACB; Compañón, I; Corzana, F; Ferhati, X; Guerreiro, A; Jiménez-Osés, G; Matos, MJ1
Cardoso, MC; Glanz, M; Hackenberger, CPR; Helma, J; Kasper, MA; Klenk, S; Krause, E; Leonhardt, H; Penkert, M; Sauer, T; Schumacher, D; Stengl, A1
Ford, A; Ruan, X; Tavares-Carreón, F; Valvano, MA1
Brun, YV; Dalia, AB; Dalia, TN; Ellison, CK1
Cook, KM1
Honjo, T; Matsuura, K1
Krzyscik, MA; Nawrocka, D; Opaliński, Ł; Otlewski, J; Zakrzewska, M1
Brik, A; Glickman, MH; Mann, G; Satish, G; Sulkshane, P; Vamisetti, GB1
Fan, J; Hu, L; Li, H; Song, Y; Sun, Y; Zhang, R; Zheng, T1
Gober, IN; Riemen, AJ; Villain, M1
Chabenne, J; DiMarchi, RD; Finan, B; He, R; Mayer, JP; Mowery, SA1
Fan, H; Guan, L; Jia, Y; Sun, H; Sun, J; Tian, J; Wang, L; Xie, C; Xue, L; Yu, D1
Gober, IN; Sharan, R; Villain, M1

Reviews

2 review(s) available for maleimide and cysteine

ArticleYear
Maleimide-based method for elaboration of cysteine-containing peptide phage libraries.
    Methods in molecular biology (Clifton, N.J.), 2015, Volume: 1248

    Topics: Animals; Cysteine; ErbB Receptors; Humans; Maleimides; Peptide Library

2015
Chemical Protein Modification through Cysteine.
    Chembiochem : a European journal of chemical biology, 2016, Apr-01, Volume: 17, Issue:7

    Topics: Cysteine; Maleimides; Protein Processing, Post-Translational; Proteins

2016

Other Studies

116 other study(ies) available for maleimide and cysteine

ArticleYear
Bivalent ACTH antagonists: influence of peptide and spacer components on potency enhancement.
    Biochemical pharmacology, 1991, Mar-01, Volume: 41, Issue:5

    Topics: Adrenal Cortex; Adrenocorticotropic Hormone; Animals; Binding Sites; Corticosterone; Cysteine; Male; Maleimides; Peptide Fragments; Rats

1991
Biotin-conjugated reagents as site-specific probes of membrane protein structure: application to the study of the human erythrocyte hexose transporter.
    Analytical biochemistry, 1990, Nov-01, Volume: 190, Issue:2

    Topics: Biotin; Carrier Proteins; Cysteine; Erythrocyte Membrane; Galactose Oxidase; Hexoses; Humans; Hydrazines; Hydrolysis; Maleimides; Membrane Proteins; Serine Endopeptidases; Trypsin

1990
Reaction of an exofacial sulfhydryl group on the erythrocyte hexose carrier with an impermeant maleimide. Relevance to the mechanism of hexose transport.
    The Journal of biological chemistry, 1988, Sep-25, Volume: 263, Issue:27

    Topics: 3-O-Methylglucose; Affinity Labels; Binding, Competitive; Biological Transport; Cysteine; Cytochalasin B; Dithiothreitol; Drug Synergism; Erythrocytes; Humans; Maleimides; Maltose; Methylglucosides; Monosaccharide Transport Proteins; Phloretin; Photochemistry; Protein Conformation; Sulfhydryl Compounds

1988
Remote conformational effects of the Gly-62 --> Leu mutation of the Tn10-encoded metal-tetracycline/H+ antiporter of Escherichia coli and its second-site suppressor mutation.
    Biochemistry, 1997, Jun-10, Volume: 36, Issue:23

    Topics: Amino Acid Sequence; Bacterial Proteins; Base Sequence; Cysteine; Escherichia coli; Glycine; Leucine; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Structure, Secondary; Restriction Mapping

1997
Specific in vivo thiol-labeling of the FhuA outer membrane ferrichrome transport protein of Escherichia coli K-12: evidence for a disulfide bridge in the predicted gating loop.
    FEMS microbiology letters, 1997, Aug-15, Volume: 153, Issue:2

    Topics: Amino Acid Sequence; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Disulfides; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Hot Temperature; Ligands; Maleimides; Molecular Sequence Data; Mutation; Oxidation-Reduction; Protein Conformation; Protein Denaturation; Receptors, Virus; Sulfhydryl Reagents

1997
Cysteine-scanning mutagenesis of the periplasmic loop regions of PomA, a putative channel component of the sodium-driven flagellar motor in Vibrio alginolyticus.
    Journal of bacteriology, 2000, Volume: 182, Issue:4

    Topics: Amino Acid Sequence; Amino Acid Substitution; Bacterial Outer Membrane Proteins; Biotin; Cysteine; Dithiothreitol; Electroporation; Flagella; Maleimides; Molecular Sequence Data; Mutagenesis; Protein Structure, Secondary; Sodium; Vibrio

2000
A novel topology model of the human Na(+)/H(+) exchanger isoform 1.
    The Journal of biological chemistry, 2000, Mar-17, Volume: 275, Issue:11

    Topics: Amino Acid Sequence; Biological Transport; Biotinylation; Cell Membrane Permeability; Cysteine; Humans; Maleimides; Mesylates; Models, Molecular; Molecular Sequence Data; Mutation; Protein Isoforms; Protein Structure, Tertiary; Sodium-Hydrogen Exchangers; Sulfhydryl Reagents; Surface Properties

2000
Topography of diphtheria Toxin's T domain in the open channel state.
    The Journal of general physiology, 2000, Volume: 115, Issue:4

    Topics: Amino Acid Sequence; Amino Acid Substitution; Biotin; Cell Membrane; Cysteine; Diphtheria Toxin; Humans; Ion Channel Gating; Ion Channels; Lipid Bilayers; Maleimides; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Conformation; Streptavidin

2000
Tethered blockers as molecular 'tape measures' for a voltage-gated K+ channel.
    Nature structural biology, 2000, Volume: 7, Issue:4

    Topics: Amino Acid Substitution; Animals; Binding Sites; Calibration; Cysteine; Drosophila melanogaster; Drosophila Proteins; Electric Conductivity; Ion Channel Gating; Ligands; Maleimides; Molecular Weight; Oocytes; Point Mutation; Potassium Channel Blockers; Potassium Channels; Protein Conformation; Quaternary Ammonium Compounds; Shaker Superfamily of Potassium Channels; Solvents; Tetraethylammonium; Xenopus laevis

2000
Constrained modeling of spin-labeled major coat protein mutants from M13 bacteriophage in a phospholipid bilayer.
    Protein science : a publication of the Protein Society, 2001, Volume: 10, Issue:5

    Topics: Amino Acid Substitution; Bacteriophage M13; Capsid; Capsid Proteins; Cysteine; Electron Spin Resonance Spectroscopy; Lipid Bilayers; Maleimides; Membrane Proteins; Models, Molecular; Mutation; Nuclear Magnetic Resonance, Biomolecular; Phospholipids; Protein Structure, Quaternary; Protein Structure, Tertiary; Spin Labels

2001
Cysteine-directed cross-linking localizes regions of the human erythrocyte anion-exchange protein (AE1) relative to the dimeric interface.
    The Biochemical journal, 2001, Nov-01, Volume: 359, Issue:Pt 3

    Topics: Amino Acid Sequence; Anion Exchange Protein 1, Erythrocyte; Biotin; Cell Line; Cross-Linking Reagents; Cysteine; Dimerization; Humans; Maleimides; Molecular Sequence Data; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Fluorescence; Transfection

2001
Kinetics of tethering quaternary ammonium compounds to K(+) channels.
    The Journal of general physiology, 2002, Volume: 120, Issue:2

    Topics: Animals; Cysteine; Kinetics; Maleimides; Oocytes; Potassium Channel Blockers; Potassium Channels; Quaternary Ammonium Compounds; Shaker Superfamily of Potassium Channels; Tetraethylammonium; Xenopus

2002
Spatial redox regulation of a critical cysteine residue of NF-kappa B in vivo.
    The Journal of biological chemistry, 2002, Nov-15, Volume: 277, Issue:46

    Topics: Cysteine; DNA; Gas Chromatography-Mass Spectrometry; Gene Expression Regulation; Humans; Jurkat Cells; Maleimides; Microscopy, Fluorescence; NF-kappa B; NF-kappa B p50 Subunit; Oxidation-Reduction; Protein Binding; Recombinant Proteins; Sulfhydryl Compounds; Time Factors; Transcription Factor RelA; Transcription, Genetic

2002
A fluorescence resonance energy transfer sensor for the beta-domain of metallothionein.
    Proceedings of the National Academy of Sciences of the United States of America, 2003, Mar-04, Volume: 100, Issue:5

    Topics: Cadmium; Circular Dichroism; Cysteine; DNA, Complementary; Escherichia coli; Fluorescence Resonance Energy Transfer; Humans; Maleimides; Metallothionein; Models, Molecular; Mutagenesis, Site-Directed; Mutation; Protein Conformation; Protein Isoforms; Protein Structure, Tertiary; Proteins; Recombinant Fusion Proteins; Recombinant Proteins; Spectrometry, Fluorescence; Sulfhydryl Compounds; Time Factors; Ultraviolet Rays; Zinc

2003
Monitoring the cellular surface display of recombinant proteins by cysteine labeling and flow cytometry.
    Chembiochem : a European journal of chemical biology, 2003, May-09, Volume: 4, Issue:5

    Topics: Adrenodoxin; Amino Acid Sequence; Animals; Bacterial Outer Membrane Proteins; Base Sequence; Biotin; Cattle; Cysteine; Escherichia coli; Escherichia coli Proteins; Flow Cytometry; Fluorescein; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Transport; Recombinant Fusion Proteins; Staining and Labeling

2003
Thiol-reactive dyes for fluorescence labeling of proteomic samples.
    Electrophoresis, 2003, Volume: 24, Issue:14

    Topics: Animals; Cysteine; Fluorescent Dyes; Humans; Iodoacetamide; Isoelectric Point; Maleimides; Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds

2003
Accessibility of cysteine residues in a cytoplasmic loop of CitS of Klebsiella pneumoniae is controlled by the catalytic state of the transporter.
    Biochemistry, 2003, Aug-19, Volume: 42, Issue:32

    Topics: Bacterial Proteins; Binding Sites; Carbon Isotopes; Carrier Proteins; Catalysis; Cell Membrane; Cell Membrane Permeability; Citric Acid; Cysteine; Cytoplasm; Ethylmaleimide; Klebsiella pneumoniae; Maleimides; Mesylates; Proton-Motive Force; Sodium; Transport Vesicles

2003
N-(4-Dimethylamino-3,5-dinitrophenyl)maleimide: a coloured sulfhydryl reagent. Isolation and investigation of cysteine-containing peptides from human and bovine serum albumin.
    Biochimica et biophysica acta, 1960, Dec-18, Volume: 45

    Topics: Albumins; Cysteine; Humans; Maleates; Maleimides; Peptides; Serum Albumin; Serum Albumin, Bovine; Sulfhydryl Compounds; Sulfhydryl Reagents

1960
Analysis of the membrane topology for transmembrane domains 7-12 of the human reduced folate carrier by scanning cysteine accessibility methods.
    The Biochemical journal, 2004, Feb-15, Volume: 378, Issue:Pt 1

    Topics: Animals; Biological Transport; Biotinylation; CHO Cells; Cricetinae; Cysteine; Humans; Maleimides; Membrane Transport Proteins; Models, Molecular; Mutagenesis, Site-Directed; Protein Structure, Tertiary; Reduced Folate Carrier Protein; Sulfhydryl Reagents

2004
About thiol derivatization and resolution of basic proteins in two-dimensional electrophoresis.
    Proteomics, 2004, Volume: 4, Issue:3

    Topics: Alkylation; Animals; Cattle; Cysteine; Cystine; Disulfides; Dithiothreitol; Electrophoresis, Gel, Two-Dimensional; Gels; Hydrogen Peroxide; Hydrogen-Ion Concentration; Iodoacetamide; Maleimides; Mass Spectrometry; Mitochondria; Peptides; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Sulfhydryl Compounds; Temperature; Time Factors; Water

2004
Relative proximity and orientation of helices 4 and 8 of the GLUT1 glucose transporter.
    The Journal of biological chemistry, 2004, Jun-18, Volume: 279, Issue:25

    Topics: Animals; Binding Sites; Cross-Linking Reagents; Cysteine; Cytoplasm; DNA, Complementary; Factor Xa; Glucose Transporter Type 1; Glycosylation; Humans; Immunoblotting; Maleimides; Models, Molecular; Monosaccharide Transport Proteins; Mutation; Oocytes; Protein Conformation; Protein Structure, Secondary; Transcription, Genetic; Xenopus laevis

2004
The topography of transmembrane segment six is altered during the catalytic cycle of P-glycoprotein.
    The Journal of biological chemistry, 2004, Aug-13, Volume: 279, Issue:33

    Topics: Adenosine Triphosphatases; Adenosine Triphosphate; Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Binding Sites; Boron Compounds; Catalysis; Cell Line; Cell Membrane; Codon; Coumarins; Cysteine; Electrophoresis, Polyacrylamide Gel; Hydrolysis; Insecta; Kinetics; Maleimides; Models, Chemical; Models, Molecular; Protein Conformation; Protein Isoforms; Protein Structure, Tertiary; Recombinant Proteins; Sequence Analysis, DNA

2004
Chemical modification of cysteine residues is a misleading indicator of their status as active site residues in the vitamin K-dependent gamma-glutamyl carboxylation reaction.
    The Journal of biological chemistry, 2004, Dec-24, Volume: 279, Issue:52

    Topics: Amino Acid Sequence; Binding Sites; Carbon-Carbon Ligases; Cysteine; Enzyme Inhibitors; Maleimides; Molecular Sequence Data; Molecular Structure; Mutagenesis, Site-Directed; Peptide Fragments; Point Mutation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Stilbenes; Structure-Activity Relationship; Sulfates; Sulfhydryl Reagents; Sulfonic Acids; Trypsin; Vitamin K

2004
Accessibility of cysteines in the native bovine rod cGMP-gated channel.
    Biochemistry, 2005, Feb-08, Volume: 44, Issue:5

    Topics: Amino Acid Sequence; Animals; Cattle; Conserved Sequence; Cyclic GMP; Cyclic Nucleotide-Gated Cation Channels; Cysteine; Ethylmaleimide; Eye Proteins; Ion Channels; Maleimides; Molecular Sequence Data; Polyethylene Glycols; Protein Subunits; Rod Cell Outer Segment; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2005
Dual-targeted labeling of proteins using cysteine and selenomethionine residues.
    Analytical biochemistry, 2005, Jul-15, Volume: 342, Issue:2

    Topics: Bacterial Proteins; Calmodulin; Cysteine; Energy Transfer; Escherichia coli; Fluorescence; Fluorescent Dyes; Hemolysin Proteins; Iodoacetamide; Maleimides; Mutagenesis, Site-Directed; Naphthalenesulfonates; Proteins; Selenomethionine

2005
Siderophore transport through Escherichia coli outer membrane receptor FhuA with disulfide-tethered cork and barrel domains.
    The Journal of biological chemistry, 2005, Aug-26, Volume: 280, Issue:34

    Topics: Bacterial Outer Membrane Proteins; Biological Transport; Blotting, Western; Cross-Linking Reagents; Cysteine; Disulfides; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Escherichia coli Proteins; Ferrichrome; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Biological; Models, Molecular; Mutation; Oxygen; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Virus; Siderophores; Sulfhydryl Compounds

2005
A mammalian actin substitution in yeast actin (H372R) causes a suppressible mitochondria/vacuole phenotype.
    The Journal of biological chemistry, 2005, Oct-28, Volume: 280, Issue:43

    Topics: Actins; Allosteric Site; Arginine; Carbon; Crystallography, X-Ray; Cysteine; Cytoskeleton; Glucose; Glycerol; Green Fluorescent Proteins; Histidine; Maleimides; Microscopy, Fluorescence; Mitochondria; Models, Molecular; Muscle, Skeletal; Muscles; Mutagenesis; Mutation; Phenotype; Polymers; Protein Conformation; Protein Structure, Tertiary; Pyrenes; Saccharomyces cerevisiae; Temperature; Time Factors; Vacuoles

2005
Role of transmembrane segment 10 in efflux mediated by the staphylococcal multidrug transport protein QacA.
    The Journal of biological chemistry, 2006, Jan-13, Volume: 281, Issue:2

    Topics: Amino Acid Sequence; Aspartic Acid; Bacterial Proteins; Binding Sites; Biological Transport; Blotting, Western; Cell Membrane; Cysteine; Drug Resistance, Multiple; Escherichia coli; Kinetics; Maleimides; Membrane Transport Proteins; Methionine; Microscopy, Fluorescence; Molecular Sequence Data; Mutagenesis; Mutation; Phenotype; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Protein Transport; Solvents; Staphylococcus aureus

2006
Oriented immobilisation of engineered single-chain antibodies to develop biosensors for virus detection.
    Journal of virological methods, 2006, Volume: 134, Issue:1-2

    Topics: Amino Acid Sequence; Antibodies, Viral; Biosensing Techniques; Comovirus; Complementarity Determining Regions; Cysteine; Genetic Vectors; Gold; Humans; Immunoglobulin Fragments; Immunoglobulin Variable Region; Maleimides; Molecular Sequence Data; Peptide Library; Plasmids; Recombinant Fusion Proteins; Surface Plasmon Resonance

2006
Development of tumor targeting anti-MUC-1 multimer: effects of di-scFv unpaired cysteine location on PEGylation and tumor binding.
    Protein engineering, design & selection : PEDS, 2006, Volume: 19, Issue:8

    Topics: Cell Line, Tumor; Chromatography, Affinity; Cysteine; Epithelial Cells; Glycosylation; Humans; Immunoglobulin Variable Region; Maleimides; Mucin-1; Polyethylene Glycols; Protein Engineering; Recombinant Proteins; Transfection

2006
On parallel and antiparallel topology of a homodimeric multidrug transporter.
    The Journal of biological chemistry, 2006, Nov-24, Volume: 281, Issue:47

    Topics: Antiporters; Cross-Linking Reagents; Cysteine; Detergents; Dimerization; Drug Resistance, Multiple; Escherichia coli Proteins; Hot Temperature; Kinetics; Maleimides; Models, Molecular; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Paraquat; Protein Binding; Protein Conformation

2006
Evidence of ball-and-chain transport of ferric enterobactin through FepA.
    The Journal of biological chemistry, 2007, Jan-05, Volume: 282, Issue:1

    Topics: Bacterial Outer Membrane Proteins; Carrier Proteins; Colicins; Cysteine; Dose-Response Relationship, Drug; Enterobactin; Escherichia coli; Fluorescein; Maleimides; Models, Molecular; Mutagenesis; Porins; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Receptors, Cell Surface; Siderophores

2007
Inactivation of oxidized and S-nitrosylated mitochondrial proteins in alcoholic fatty liver of rats.
    Hepatology (Baltimore, Md.), 2006, Volume: 44, Issue:5

    Topics: Animals; Central Nervous System Depressants; Cysteine; Cytochrome P-450 CYP2E1; Ethanol; Fatty Liver, Alcoholic; Isoenzymes; Male; Maleimides; Mitochondria, Liver; Mitochondrial Proteins; Nitric Oxide Synthase; Nitrites; Nitrosation; Oxidation-Reduction; Oxidative Stress; Rats; Rats, Sprague-Dawley; Sequence Analysis, Protein

2006
A periplasmic coiled-coil interface underlying TolC recruitment and the assembly of bacterial drug efflux pumps.
    Proceedings of the National Academy of Sciences of the United States of America, 2007, Mar-13, Volume: 104, Issue:11

    Topics: Bacterial Outer Membrane Proteins; Cross-Linking Reagents; Cysteine; Drug Resistance, Bacterial; Escherichia coli; Escherichia coli Proteins; Genetic Variation; Lipoproteins; Maleimides; Membrane Transport Proteins; Models, Molecular; Multidrug Resistance-Associated Proteins; Periplasm; Protein Binding; Protein Conformation; Protein Structure, Secondary; Pseudomonas aeruginosa

2007
Mapping the electrostatic potential within the ribosomal exit tunnel.
    Journal of molecular biology, 2007, Aug-31, Volume: 371, Issue:5

    Topics: Amino Acid Sequence; Bacterial Proteins; Cysteine; Kinetics; Maleimides; Microtubule-Associated Proteins; Models, Molecular; Molecular Conformation; Molecular Sequence Data; Polyethylene Glycols; Protein Biosynthesis; Protein Conformation; Protein Folding; Ribosomes; Static Electricity

2007
Anti-parallel membrane topology of a homo-dimeric multidrug transporter, EmrE.
    Journal of biochemistry, 2007, Volume: 142, Issue:5

    Topics: Antiporters; Biological Transport; Biotin; Cell Membrane Permeability; Cysteine; Cytoplasm; Dimerization; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Multidrug Resistance-Associated Proteins; Mutation; Periplasm; Polyethylene; Proline; Protein Structure, Secondary; Threonine

2007
Porphyrinmaleimides: towards thiol probes for cysteine residues in proteins.
    Bioconjugate chemistry, 2008, Volume: 19, Issue:1

    Topics: Cysteine; Electrophoresis, Polyacrylamide Gel; Light; Maleimides; Porphyrins; Rhodopsin; Sulfhydryl Compounds; Ultraviolet Rays

2008
Evaluation of maleimide derivative of DOTA for site-specific labeling of recombinant affibody molecules.
    Bioconjugate chemistry, 2008, Volume: 19, Issue:1

    Topics: Animals; Binding Sites; Chelating Agents; Cysteine; Heterocyclic Compounds, 1-Ring; Histidine; Indium Radioisotopes; Maleimides; Mice; Mice, Inbred BALB C; Neoplasms; Peptides; Radionuclide Imaging; Receptor, ErbB-2; Recombinant Fusion Proteins; Sensitivity and Specificity; Transplantation, Heterologous

2008
Rapid identification of reactive cysteine residues for site-specific labeling of antibody-Fabs.
    Journal of immunological methods, 2008, Mar-20, Volume: 332, Issue:1-2

    Topics: Antibodies, Monoclonal; Antigen-Antibody Reactions; Binding Sites; Biotin; Cell Line, Tumor; Chromatography, Liquid; Cysteine; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation; Genetic Engineering; Genetic Variation; Humans; Immunoglobulin Fab Fragments; Maleimides; Mass Spectrometry; Models, Molecular; Mutagenesis, Site-Directed; Plasmids; Sensitivity and Specificity; Staining and Labeling; Surface Properties

2008
Recombinant aequorin with a reactive cysteine residue for conjugation with maleimide-activated antibody.
    Analytical biochemistry, 2008, Jul-01, Volume: 378, Issue:1

    Topics: Aequorin; Amino Acid Sequence; Antibodies; Biosensing Techniques; Cysteine; Genetic Vectors; Luminescent Measurements; Maleimides; Molecular Sequence Data; Recombinant Proteins

2008
Serial protein labeling with infrared maleimide dyes to identify cysteine modifications.
    Journal of proteomics, 2008, Jul-21, Volume: 71, Issue:2

    Topics: Affinity Labels; Aged; Cysteine; Fluorescent Dyes; Humans; Male; Maleimides; Nerve Tissue Proteins; Proteomics; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Staining and Labeling; Sulfhydryl Compounds

2008
Probing conformational changes of prestin with thiol-reactive optical switches.
    Biophysical journal, 2008, Sep-15, Volume: 95, Issue:6

    Topics: Animals; Anion Transport Proteins; Biotin; Cell Membrane; Cysteine; Electric Capacitance; Guinea Pigs; Hair Cells, Auditory, Outer; Humans; Iodoacetamide; Maleimides; Optics and Photonics; Protein Conformation; Sulfhydryl Compounds; Transfection

2008
In situ synthesis of lipopeptides as versatile receptors for the specific binding of nanoparticles and liposomes to solid-supported membranes.
    Small (Weinheim an der Bergstrasse, Germany), 2008, Volume: 4, Issue:7

    Topics: Cysteine; Diffusion; Lipid Bilayers; Liposomes; Lysine; Maleimides; Micelles; Microscopy, Confocal; Models, Chemical; Nanoparticles; Nanotechnology; Peptides; Phospholipids; Static Electricity; Water

2008
Molecular bases for the recognition of short peptide substrates and cysteine-directed modifications of human insulin-degrading enzyme.
    Biochemistry, 2008, Dec-02, Volume: 47, Issue:48

    Topics: Alkylation; Amino Acid Substitution; Catalytic Domain; Conserved Sequence; Crystallography, X-Ray; Cysteine; Enzyme Inhibitors; Humans; Insulysin; Kallidin; Maleimides; Models, Molecular; Oxidation-Reduction; Peptides; Protein Processing, Post-Translational; Substrate Specificity; Sulfhydryl Compounds

2008
Simple ortho- and para-hydroquinones as compounds neuroprotective against oxidative stress in a manner associated with specific transcriptional activation.
    Biochemical and biophysical research communications, 2009, Feb-06, Volume: 379, Issue:2

    Topics: Abietanes; Animals; Antioxidants; Cattle; Cell Line; Cysteine; Fluorescent Dyes; Glutathione; Humans; Hydroquinones; Maleimides; Neurons; Neuroprotective Agents; Oxidative Stress; Plant Extracts; Response Elements; Serum Albumin, Bovine; Transcriptional Activation

2009
Targeting of epidermal growth factor receptor (EGFR)-expressing tumor cells with sterically stabilized affibody liposomes (SAL).
    Bioconjugate chemistry, 2009, Volume: 20, Issue:6

    Topics: Antibodies; Biological Transport; Cell Line, Tumor; Cysteine; ErbB Receptors; Gene Expression Regulation, Neoplastic; Humans; Inhibitory Concentration 50; Liposomes; Maleimides; Mitoxantrone; Nanostructures; Neoplasms; Phosphatidylethanolamines; Polyethylene Glycols; Recombinant Fusion Proteins; Substrate Specificity

2009
Synthesis of semitelechelic maleimide poly(PEGA) for protein conjugation by RAFT polymerization.
    Biomacromolecules, 2009, Jul-13, Volume: 10, Issue:7

    Topics: Chromatography, Gel; Cysteine; Electrophoresis, Polyacrylamide Gel; Maleimides; Molecular Weight; Muramidase; Polyethylene Glycols; Polymerization; Proteins

2009
Bromomaleimides: new reagents for the selective and reversible modification of cysteine.
    Chemical communications (Cambridge, England), 2009, Nov-21, Issue:43

    Topics: Bromine; Cysteine; Indicators and Reagents; Maleimides

2009
Probing the orientation of surface-immobilized protein G B1 using ToF-SIMS, sum frequency generation, and NEXAFS spectroscopy.
    Langmuir : the ACS journal of surfaces and colloids, 2010, Nov-02, Volume: 26, Issue:21

    Topics: Adsorption; Cysteine; Ethylene Glycol; Gold; Hydrogen-Ion Concentration; Maleimides; Molecular Structure; Mutation; Nerve Tissue Proteins; Particle Size; Spectrometry, Mass, Secondary Ion; Spectrum Analysis; Sulfhydryl Compounds; Surface Properties

2010
De novo helical peptides as target sequences for a specific, fluorogenic protein labelling strategy.
    Molecular bioSystems, 2010, Volume: 6, Issue:6

    Topics: Amino Acid Sequence; Cell Line; Circular Dichroism; Cysteine; ErbB Receptors; Fluorescent Dyes; Humans; Maleimides; Maltose-Binding Proteins; Microscopy, Confocal; Models, Chemical; Models, Molecular; Molecular Sequence Data; Molecular Structure; Peptides; Periplasmic Binding Proteins; Protein Structure, Secondary; Proteins; Proto-Oncogene Proteins c-fos; Staining and Labeling

2010
A maleimide-based in-vitro model for ultrasound targeted imaging.
    Ultrasonics sonochemistry, 2011, Volume: 18, Issue:1

    Topics: Cysteine; Gelatin; Maleimides; Models, Chemical; Surface Properties; Ultrasonics

2011
CYP2C8 exists as a dimer in natural membranes.
    Drug metabolism and disposition: the biological fate of chemicals, 2010, Volume: 38, Issue:11

    Topics: Amino Acid Sequence; Aryl Hydrocarbon Hydroxylases; Binding Sites; Cell Line; Cross-Linking Reagents; Cysteine; Cytochrome P-450 CYP2C8; Electrophoresis, Polyacrylamide Gel; Endoplasmic Reticulum; Escherichia coli; Humans; Intracellular Membranes; Maleimides; Models, Molecular; Molecular Sequence Data; Protein Conformation; Protein Multimerization; Transfection

2010
Solvent effect and time-dependent behavior of C-terminus-cysteine-modified cecropin P1 chemically immobilized on a polymer surface.
    Langmuir : the ACS journal of surfaces and colloids, 2011, Jun-07, Volume: 27, Issue:11

    Topics: Amino Acid Sequence; Buffers; Cysteine; Immobilized Proteins; Kinetics; Maleimides; Models, Molecular; Molecular Sequence Data; Peptides; Phosphates; Polystyrenes; Potassium Compounds; Protein Multimerization; Protein Structure, Quaternary; Reducing Agents; Solutions; Solvents; Surface Properties; Trifluoroethanol

2011
Quantification of cysteinyl S-nitrosylation by fluorescence in unbiased proteomic studies.
    Biochemistry, 2011, Jun-28, Volume: 50, Issue:25

    Topics: Animals; Boron Compounds; Calorimetry; Cysteine; Female; Fluorescence; Hypoxia; Ischemia; Luminescence; Maleimides; Nitric Oxide; Perfusion; Phosphorylation; Proteomics; Random Allocation; Rats; Rats, Wistar

2011
Site-specific labeling of proteins for single-molecule FRET measurements using genetically encoded ketone functionalities.
    Methods in molecular biology (Clifton, N.J.), 2011, Volume: 751

    Topics: Bacteriophage T4; Binding Sites; Cysteine; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Hydroxylamine; Ketones; Maleimides; Muramidase; Oximes; Phenylalanine; Protein Engineering; Recombinant Proteins; Staining and Labeling; Transformation, Genetic

2011
Identification of anchor points for chemical modification of a small cysteine-rich protein by using a cysteine scan.
    Chembiochem : a European journal of chemical biology, 2011, Nov-04, Volume: 12, Issue:16

    Topics: Alkylation; Animals; Chromatography, High Pressure Liquid; Cysteine; Disulfides; Insulin; Male; Maleimides; Polyethylene Glycols; Rats; Rats, Wistar; Stereoisomerism; Tandem Mass Spectrometry

2011
Maleimide-functionalised organoruthenium anticancer agents and their binding to thiol-containing biomolecules.
    Chemical communications (Cambridge, England), 2012, Feb-01, Volume: 48, Issue:10

    Topics: Acetylcysteine; Antineoplastic Agents; Binding Sites; Cell Line, Tumor; Cell Proliferation; Cysteine; Dose-Response Relationship, Drug; Glutathione; Humans; Maleimides; Models, Molecular; Molecular Structure; Organometallic Compounds; Ruthenium; Structure-Activity Relationship; Sulfhydryl Compounds

2012
The signal-anchor sequence of CYP2C1 inserts into the membrane as a hairpin structure.
    Biochemical and biophysical research communications, 2011, Nov-18, Volume: 415, Issue:2

    Topics: Amino Acid Sequence; Animals; Cell Membrane; Chlorocebus aethiops; COS Cells; Cysteine; Cytochrome P-450 CYP2E1; Cytochrome P-450 CYP3A; Cytochrome P-450 Enzyme System; HEK293 Cells; Humans; Maleimides; Molecular Sequence Data; Polyethylene Glycols; Protein Sorting Signals; Protein Structure, Secondary

2011
A chromenoquinoline-based fluorescent off-on thiol probe for bioimaging.
    Chemical communications (Cambridge, England), 2012, Mar-11, Volume: 48, Issue:21

    Topics: Benzopyrans; Cell Line, Tumor; Cell Membrane Permeability; Cysteine; Fluorescent Dyes; Humans; Maleimides; Microscopy, Fluorescence; Quantum Theory; Quinolines; Sulfhydryl Compounds

2012
Site-specific fluorescent labeling and oriented immobilization of a triple mutant of CYP3A4 via C64.
    Bioconjugate chemistry, 2012, Apr-18, Volume: 23, Issue:4

    Topics: Amino Acid Sequence; Binding Sites; Cysteine; Cytochrome P-450 CYP3A; Enzymes, Immobilized; Fluorescent Dyes; Humans; Maleimides; Microspheres; Models, Molecular; Molecular Sequence Data; Mutant Proteins; Mutation; Protein Conformation; Silicon Dioxide; Substrate Specificity

2012
Real-time sensing and discrimination of single chemicals using the channel of phi29 DNA packaging nanomotor.
    ACS nano, 2012, Apr-24, Volume: 6, Issue:4

    Topics: Bacillus Phages; Benzene; Biosensing Techniques; Cysteine; DNA Packaging; DNA, Viral; Ethane; Maleimides; Models, Molecular; Mutation; Nanopores; Nucleic Acid Conformation; Protein Conformation; Thymine; Time Factors; Viral Proteins

2012
Purification of histidine-tagged aequorin with a reactive cysteine residue for chemical conjugations and its application for bioluminescent sandwich immunoassays.
    Protein expression and purification, 2012, Volume: 83, Issue:2

    Topics: Aequorin; alpha-Fetoproteins; Biotin; Cysteine; Histidine; Immunoassay; Luminescent Measurements; Maleimides; Models, Chemical; Recombinant Fusion Proteins; Sensitivity and Specificity; Streptavidin

2012
Different functions of MdtB and MdtC subunits in the heterotrimeric efflux transporter MdtB(2)C complex of Escherichia coli.
    Biochemistry, 2012, May-22, Volume: 51, Issue:20

    Topics: Amino Acid Sequence; Biological Transport; Cloxacillin; Cysteine; Escherichia coli Proteins; Maleimides; Membrane Transport Proteins; Models, Molecular; Molecular Sequence Data; Multidrug Resistance-Associated Proteins; Mutagenesis, Site-Directed; Protein Conformation; Protein Multimerization

2012
A simple, rapid and inexpensive technique to bind small peptides to polystyrene surfaces for immunoenzymatic assays.
    Journal of immunological methods, 2012, Aug-31, Volume: 382, Issue:1-2

    Topics: Antibodies; Carrier Proteins; Cysteine; Enzyme-Linked Immunosorbent Assay; Maleimides; Peptides; Polystyrenes; Surface Properties

2012
Involvement of the cysteine-rich head domain in activation and desensitization of the P2X1 receptor.
    Proceedings of the National Academy of Sciences of the United States of America, 2012, Jul-10, Volume: 109, Issue:28

    Topics: Animals; Cations; Cell Membrane; Crystallography, X-Ray; Cysteine; DNA, Complementary; Electrophysiology; Kinetics; Maleimides; Microscopy, Fluorescence; Mutation; Oocytes; Protein Binding; Protein Structure, Tertiary; Rats; Receptors, Purinergic P2X1; Rhodamines; Xenopus; Xenopus laevis

2012
Electrochemical functionalization of gold and silicon surfaces by a maleimide group as a biosensor for immunological application.
    Acta biomaterialia, 2013, Volume: 9, Issue:3

    Topics: Antibodies; Biosensing Techniques; Cysteine; Electrochemical Techniques; Electrodes; Gold; Immobilized Proteins; Maleimides; Peptides; Silicon; Spectrophotometry, Infrared; Surface Properties

2013
Rhodium-catalyzed cysteine modification with diazo reagents.
    Chemical communications (Cambridge, England), 2013, May-14, Volume: 49, Issue:39

    Topics: Azo Compounds; Catalysis; Coordination Complexes; Cysteine; Cystic Fibrosis Transmembrane Conductance Regulator; Humans; Maleimides; Rhodium

2013
Straightforward synthesis of cyclic and bicyclic peptides.
    Organic letters, 2013, Apr-19, Volume: 15, Issue:8

    Topics: Cyclization; Cysteine; Maleimides; Molecular Structure; Peptides; Peptides, Cyclic

2013
On-chip enrichment and analysis of peptide subsets using a maleimide-functionalized fluorous affinity biochip and nanostructure initiator mass spectrometry.
    Analytical chemistry, 2013, Jun-04, Volume: 85, Issue:11

    Topics: Affinity Labels; Cysteine; Fluorocarbons; Maleimides; Microarray Analysis; Nanostructures; Peptide Fragments; Serum Albumin, Bovine; Silicon; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Tandem Mass Spectrometry

2013
Convenient method to assess chemical modification of protein thiols by electrophilic metals.
    The Journal of toxicological sciences, 2013, Volume: 38, Issue:3

    Topics: Adaptor Proteins, Signal Transducing; Animals; Biotin; Cysteine; Cytoskeletal Proteins; Electrophoresis, Polyacrylamide Gel; Hepatocytes; Humans; Kelch-Like ECH-Associated Protein 1; Male; Maleimides; Methylmercury Compounds; Mice; Mice, Inbred C57BL; Neuroblastoma; Sulfhydryl Compounds; Tumor Cells, Cultured

2013
Characterization of a site-specific PEGylated analog of exendin-4 and determination of the PEGylation site.
    International journal of pharmaceutics, 2013, Sep-15, Volume: 454, Issue:1

    Topics: Amino Acid Sequence; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Cysteine; Electrophoresis, Polyacrylamide Gel; Exenatide; Maleimides; Molecular Sequence Data; Peptide Mapping; Peptides; Polyethylene Glycols; Protein Conformation; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Technology, Pharmaceutical; Venoms

2013
Molecular orientation of enzymes attached to surfaces through defined chemical linkages at the solid-liquid interface.
    Journal of the American Chemical Society, 2013, Aug-28, Volume: 135, Issue:34

    Topics: Binding Sites; Cysteine; Enzymes, Immobilized; Ethylene Glycol; Glycoside Hydrolases; Lactococcus lactis; Maleimides; Models, Molecular; Surface Properties

2013
Covalent trapping of methyllycaconitine at the α4-α4 interface of the α4β2 nicotinic acetylcholine receptor: antagonist binding site and mode of receptor inhibition revealed.
    The Journal of biological chemistry, 2013, Sep-13, Volume: 288, Issue:37

    Topics: Aconitine; Animals; Binding Sites; Cysteine; Escherichia coli; Female; Ligands; Maleimides; Mutagenesis, Site-Directed; Nicotinic Antagonists; Oocytes; Protein Binding; Protein Conformation; Rats; Receptors, Nicotinic; Recombinant Proteins; Xenopus laevis

2013
A simple and efficient maleimide-based approach for peptide extension with a cysteine-containing peptide phage library.
    Bioorganic & medicinal chemistry letters, 2013, Oct-15, Volume: 23, Issue:20

    Topics: Amino Acid Sequence; Cysteine; ErbB Receptors; Humans; Maleimides; Molecular Sequence Data; Peptide Library; Peptides; Protein Binding; Recombinant Fusion Proteins

2013
Rapid, stable, chemoselective labeling of thiols with Julia-Kocieński-like reagents: a serum-stable alternative to maleimide-based protein conjugation.
    Angewandte Chemie (International ed. in English), 2013, Nov-25, Volume: 52, Issue:48

    Topics: Click Chemistry; Cysteine; Humans; Maleimides; Models, Molecular; Oxadiazoles; Plasma; Protein Stability; Proteins; Sulfhydryl Compounds

2013
Molecular structures of C- and N-terminus cysteine modified cecropin P1 chemically immobilized onto maleimide-terminated self-assembled monolayers investigated by molecular dynamics simulation.
    The journal of physical chemistry. B, 2014, May-29, Volume: 118, Issue:21

    Topics: Amino Acid Sequence; Anti-Infective Agents; Cysteine; Immobilized Proteins; Maleimides; Molecular Dynamics Simulation; Peptides; Protein Structure, Secondary; Surface Properties; Vibration; Water

2014
Two-photon fluorescent probe derived from naphthalimide for cysteine detection and imaging in living cells.
    Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 2015, Feb-25, Volume: 137

    Topics: Cysteine; Fluorescent Dyes; Glutathione; HeLa Cells; Homocysteine; Humans; Magnetic Resonance Spectroscopy; Maleimides; Models, Chemical; Molecular Probe Techniques; Naphthalimides; Photons; Sensitivity and Specificity; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Sulfhydryl Compounds

2015
Grafting synthetic transmembrane units to the engineered low-toxicity α-hemolysin to restore its hemolytic activity.
    Molecular bioSystems, 2014, Volume: 10, Issue:12

    Topics: Amino Acid Sequence; Animals; Bacterial Proteins; Bacterial Toxins; Cysteine; Erythrocytes; Fluoresceins; Gene Expression Regulation; Hemolysin Proteins; Hemolysis; Hydrogen-Ion Concentration; Maleimides; Microscopy, Electron, Transmission; Molecular Sequence Data; Molecular Structure; Mutation; Protein Conformation; Protein Engineering; Sheep; Staphylococcus aureus

2014
Direct determination of the redox status of cysteine residues in proteins in vivo.
    Biochemical and biophysical research communications, 2015, Jan-02, Volume: 456, Issue:1

    Topics: Arabidopsis; Blotting, Western; Cysteine; DNA; Electrophoresis, Gel, Two-Dimensional; Escherichia coli; HeLa Cells; Humans; Maleimides; Oxidation-Reduction; Oxidative Stress; Proteins; Sulfhydryl Compounds; Ultraviolet Rays

2015
Application of multiplexed cysteine-labeled complex protein sample for 2D electrophoretic gel alignment.
    Proteomics, 2015, Volume: 15, Issue:11

    Topics: Benzopyrans; Cysteine; Electrophoresis, Gel, Two-Dimensional; Fluorescent Dyes; Image Processing, Computer-Assisted; Indoles; Maleimides; Malus; Pilot Projects; Plant Proteins; Software

2015
Site-specific covalent attachment of an engineered Z-domain onto a solid matrix: an efficient platform for 3D IgG immobilization.
    Analytica chimica acta, 2015, May-04, Volume: 872

    Topics: Antibodies, Immobilized; Chromatography, Affinity; Chromatography, Gel; Cysteine; Immunoglobulin G; Maleimides; Peptides; Polystyrenes; Protein Engineering; Protein Structure, Tertiary; Surface Properties

2015
Cys34-PEGylated Human Serum Albumin for Drug Binding and Delivery.
    Bioconjugate chemistry, 2015, May-20, Volume: 26, Issue:5

    Topics: Cell Survival; Chemistry, Pharmaceutical; Cysteine; Drug Carriers; Humans; Maleimides; MCF-7 Cells; Models, Molecular; Nanoparticles; Paclitaxel; Permeability; Polyethylene Glycols; Protein Conformation; Serum Albumin

2015
Identification of highly reactive cysteine residues at less exposed positions in the Fab constant region for site-specific conjugation.
    Bioconjugate chemistry, 2015, Jun-17, Volume: 26, Issue:6

    Topics: Cell Line, Tumor; Cysteine; Escherichia coli; Humans; Immunoconjugates; Immunoglobulin Fab Fragments; Maleimides; Polyethylene Glycols; Protein Engineering; Protein Stability; Sulfhydryl Compounds

2015
Quantification of PEG-maleimide ligands and coupling efficiencies on nanoparticles with Ellman's reagent.
    Analytical chemistry, 2015, Sep-15, Volume: 87, Issue:18

    Topics: Coloring Agents; Cysteine; Dithionitrobenzoic Acid; Hydrogen-Ion Concentration; Ligands; Maleimides; Nanoparticles; Polyethylene Glycols; Polystyrenes

2015
Nanometric distance measurements between Mn(ii)DOTA centers.
    Physical chemistry chemical physics : PCCP, 2015, Sep-28, Volume: 17, Issue:36

    Topics: Coordination Complexes; Cysteine; Electron Spin Resonance Spectroscopy; Heterocyclic Compounds, 1-Ring; Maleimides; Manganese; Models, Molecular; Peptides; Protein Structure, Secondary; Spin Labels

2015
Oriented Peptide Immobilization on Microspheres.
    Methods in molecular biology (Clifton, N.J.), 2016, Volume: 1352

    Topics: Amines; Antimicrobial Cationic Peptides; Carbodiimides; Cysteine; Immobilized Proteins; Maleimides; Microspheres; Polyethylene Glycols; Protein Array Analysis; Protein Structure, Secondary; Succinimides; Surface Properties

2016
Chicken scFvs with an Artificial Cysteine for Site-Directed Conjugation.
    PloS one, 2016, Volume: 11, Issue:1

    Topics: Amino Acid Substitution; Animals; Chickens; Cysteine; Immunoconjugates; Immunoglobulin kappa-Chains; Maleimides; Mutagenesis, Site-Directed; Mutation; Recombinant Fusion Proteins; Single-Chain Antibodies; Sulfhydryl Compounds

2016
Rational Design of an Ultrasensitive and Highly Selective Chemodosimeter by a Dual Quenching Mechanism for Cysteine Based on a Facile Michael-Transcyclization Cascade Reaction.
    Chemistry (Weinheim an der Bergstrasse, Germany), 2016, Jun-27, Volume: 22, Issue:27

    Topics: Click Chemistry; Cyclization; Cysteine; Electron Transport; Fluorescent Dyes; Glutathione; HeLa Cells; Homocysteine; Humans; Limit of Detection; Maleimides; Microscopy, Fluorescence; Quantum Theory; Spectrometry, Fluorescence

2016
Natural Product Splicing Inhibitors: A New Class of Antibody-Drug Conjugate (ADC) Payloads.
    Bioconjugate chemistry, 2016, 08-17, Volume: 27, Issue:8

    Topics: Animals; ATP Binding Cassette Transporter, Subfamily B, Member 1; Biological Products; Carboxylic Acids; Cell Line, Tumor; Cell Transformation, Neoplastic; Cysteine; Humans; Immunoconjugates; Lysine; Maleimides; Mice; Pyrans; Tissue Distribution

2016
Sulfo-SMCC Prevents Annealing of Taxol-Stabilized Microtubules In Vitro.
    PloS one, 2016, Volume: 11, Issue:8

    Topics: Animals; Antineoplastic Agents, Phytogenic; Cysteine; Esters; Maleimides; Microtubules; Paclitaxel; Polymers; Succinimides; Swine; Tubulin

2016
Highly sensitive detection of cysteine over glutathione and homo-cysteine: New insight into the Michael addition of mercapto group to maleimide.
    Biosensors & bioelectronics, 2017, May-15, Volume: 91

    Topics: Animals; Biosensing Techniques; Cattle; Cell Line; Coumarins; Cysteine; Fluorescent Dyes; Glutathione; Homocysteine; Limit of Detection; Maleimides; Mice; Models, Molecular; Optical Imaging; Serum Albumin, Bovine; Spectrometry, Fluorescence; Sulfhydryl Compounds

2017
Engineering and Characterization of an Enzyme Replacement Therapy for Classical Homocystinuria.
    Biomacromolecules, 2017, Jun-12, Volume: 18, Issue:6

    Topics: Amino Acid Sequence; Animals; Cross-Linking Reagents; Cystathionine beta-Synthase; Cysteine; Delayed-Action Preparations; Disease Models, Animal; Enzyme Replacement Therapy; Homocysteine; Homocystinuria; Humans; Maleimides; Mice; Polyethylene Glycols; Succinimides

2017
Development of a Small Hybrid Molecule That Mediates Degradation of His-Tag Fused Proteins.
    Journal of medicinal chemistry, 2018, 01-25, Volume: 61, Issue:2

    Topics: Biotin; Cell Line; Cysteine; Histidine; Humans; Leupeptins; Maleimides; Nitrilotriacetic Acid; Organometallic Compounds; Proteasome Inhibitors; Proteolysis; Receptors, Retinoic Acid; Recombinant Fusion Proteins; Smad2 Protein; Ubiquitin-Protein Ligases

2018
Mass-Tag Labeling Using Acyl-PEG Exchange for the Determination of Endogenous Protein S-Fatty Acylation.
    Current protocols in protein science, 2017, 08-01, Volume: 89

    Topics: Acrylates; Acylation; Cell Line; Cysteine; Fatty Acids; HEK293 Cells; Humans; Maleimides; Polyethylene Glycols; Protein Binding; Proteins

2017
Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific Labeling.
    Methods in enzymology, 2017, Volume: 594

    Topics: Biochemistry; Biological Transport; Carrier Proteins; Cross-Linking Reagents; Cysteine; Electrophoresis, Polyacrylamide Gel; Maleimides; Mass Spectrometry; Mercury; Mesylates; Protein Conformation

2017
A thiol probe for measuring unfolded protein load and proteostasis in cells.
    Nature communications, 2017, 09-07, Volume: 8, Issue:1

    Topics: Animals; Artemisinins; Cells; Cysteine; Fluorescent Dyes; HEK293 Cells; HeLa Cells; Humans; Huntingtin Protein; Malaria; Maleimides; Mice; Molecular Probes; Mutant Proteins; Oligopeptides; Parasites; Protein Folding; Proteome; Proteostasis; Solubility; Sulfhydryl Compounds; Tunicamycin

2017
Attachment Site Cysteine Thiol pK
    Bioconjugate chemistry, 2017, 10-18, Volume: 28, Issue:10

    Topics: Benzodiazepines; Cysteine; Drug Stability; Immunoconjugates; Maleimides; Models, Molecular; Mutation; Protein Conformation; Pyrroles

2017
Paper-based fluorescent sensor via aggregation induced emission fluorogen for facile and sensitive visual detection of hydrogen peroxide and glucose.
    Biosensors & bioelectronics, 2018, May-01, Volume: 104

    Topics: Biosensing Techniques; Blood Glucose; Cysteine; Fluorescent Dyes; Glucose Oxidase; Humans; Hydrogen Peroxide; Limit of Detection; Maleimides; Oxidation-Reduction; Reactive Oxygen Species; Stilbenes

2018
High-Throughput Cysteine Scanning To Identify Stable Antibody Conjugation Sites for Maleimide- and Disulfide-Based Linkers.
    Bioconjugate chemistry, 2018, 02-21, Volume: 29, Issue:2

    Topics: Animals; Antineoplastic Agents, Immunological; Cysteine; Disulfides; Drug Stability; High-Throughput Screening Assays; Humans; Immunoconjugates; Maleimides; Models, Molecular; Mutagenesis, Site-Directed; Oligopeptides; Protein Aggregates; Protein Stability; Rats; Trastuzumab

2018
Site-Specific Three-Color Labeling of α-Synuclein via Conjugation to Uniquely Reactive Cysteines during Assembly by Native Chemical Ligation.
    Cell chemical biology, 2018, 06-21, Volume: 25, Issue:6

    Topics: alpha-Synuclein; Color; Cysteine; Fluorescence Resonance Energy Transfer; Fluorescent Dyes; Humans; Maleimides; Protein Conformation

2018
A Two-Step Immunocapture LC/MS/MS Assay for Plasma Stability and Payload Migration Assessment of Cysteine-Maleimide-Based Antibody Drug Conjugates.
    Analytical chemistry, 2018, 05-15, Volume: 90, Issue:10

    Topics: Albumins; Animals; Antibodies, Monoclonal; Antibodies, Monoclonal, Humanized; Chromatography, Liquid; Cysteine; Humans; Immunoassay; Immunoconjugates; Maleimides; Mice; Tandem Mass Spectrometry

2018
Dual Functionalization of Rod-Shaped Viruses on Single Coat Protein Subunits.
    Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1776

    Topics: Amino Acid Sequence; Capsid Proteins; Cysteine; Maleimides; Nanotechnology; Nicotiana; Protein Subunits; RNA, Viral; Succinimides; Sulfhydryl Compounds; Tobacco Mosaic Virus

2018
Characterization of Ring-Opening Reaction of Succinimide Linkers in ADCs.
    Journal of pharmaceutical sciences, 2019, Volume: 108, Issue:1

    Topics: Antibodies, Monoclonal; Antineoplastic Agents; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Cysteine; Hydrolysis; Immunoconjugates; Maleimides; Succinimides; Sulfhydryl Compounds

2019
Efficient and irreversible antibody-cysteine bioconjugation using carbonylacrylic reagents.
    Nature protocols, 2019, Volume: 14, Issue:1

    Topics: Acrylates; Analytic Sample Preparation Methods; Chromatography, Liquid; Cross-Linking Reagents; Cysteine; Humans; Immunoconjugates; Immunoglobulins; Maleimides; Single-Domain Antibodies; Tandem Mass Spectrometry

2019
Cysteine-Selective Phosphonamidate Electrophiles for Modular Protein Bioconjugations.
    Angewandte Chemie (International ed. in English), 2019, 08-19, Volume: 58, Issue:34

    Topics: Antineoplastic Agents, Immunological; Cysteine; Humans; Immunoconjugates; Iodoacetamide; Maleimides; Organophosphonates; Receptor, ErbB-2; Sulfhydryl Compounds; Trastuzumab

2019
Sulfhydryl Labeling as a Tool to Investigate the Topology of Membrane Proteins Involved in Lipopolysaccharide Biosynthesis.
    Methods in molecular biology (Clifton, N.J.), 2019, Volume: 1954

    Topics: Bacteria; Bacterial Proteins; Biosynthetic Pathways; Cysteine; Electrophoresis, Polyacrylamide Gel; Ethylmaleimide; Genes, Bacterial; Lipopolysaccharides; Maleimides; Mutagenesis, Site-Directed; Polyethylene Glycols; Staining and Labeling; Sulfhydryl Compounds

2019
Real-time microscopy and physical perturbation of bacterial pili using maleimide-conjugated molecules.
    Nature protocols, 2019, Volume: 14, Issue:6

    Topics: Biotin; Caulobacter; Cysteine; Fimbriae, Bacterial; Fluorescent Dyes; Maleimides; Microscopy, Fluorescence; Models, Molecular; Optical Imaging; Staining and Labeling; Vibrio cholerae

2019
Determining the Redox Potential of a Protein Disulphide Bond.
    Methods in molecular biology (Clifton, N.J.), 2019, Volume: 1967

    Topics: Biotin; Cysteine; Disulfides; Maleimides; Oxidation-Reduction; Protein Domains; Protein Folding; Proteins; Sulfhydryl Compounds; Tandem Mass Spectrometry

2019
Artificial Viral Capsid Dressed Up with Human Serum Albumin.
    Bioconjugate chemistry, 2019, 06-19, Volume: 30, Issue:6

    Topics: Capsid; Cross-Linking Reagents; Cysteine; Humans; Maleimides; Models, Molecular; Nanocapsules; Peptides; Serum Albumin, Human

2019
Stable Fibroblast Growth Factor 2 Dimers with High Pro-Survival and Mitogenic Potential.
    International journal of molecular sciences, 2020, Jun-09, Volume: 21, Issue:11

    Topics: Animals; Apoptosis; Cell Line, Tumor; Cell Movement; Cysteine; Fibroblast Growth Factor 2; Heparin; Humans; Maleimides; Mice; Mitogens; Mitosis; NIH 3T3 Cells; Osteosarcoma; Polyethylene Glycols; Protein Engineering; Protein Multimerization; Receptor, Fibroblast Growth Factor, Type 1; Recombinant Proteins

2020
On-Demand Detachment of Succinimides on Cysteine to Facilitate (Semi)Synthesis of Challenging Proteins.
    Journal of the American Chemical Society, 2020, 11-18, Volume: 142, Issue:46

    Topics: Catalysis; Coordination Complexes; Cycloaddition Reaction; Cysteine; Disulfides; Globins; Inteins; Maleimides; Palladium; Peptides; Proteins; Solid-Phase Synthesis Techniques; Succinimides; Thiazolidines; Thioredoxins; Ubiquitin; Ubiquitin Thiolesterase

2020
Ultrasensitive and selective fluorescent sensor for cysteine and application to drug analysis and bioimaging.
    Analytical biochemistry, 2021, 05-01, Volume: 620

    Topics: Coumarins; Cysteine; Fluorescent Dyes; HeLa Cells; Humans; Maleimides; Molecular Structure; Optical Imaging

2021
Sequence sensitivity and pH dependence of maleimide conjugated N-terminal cysteine peptides to thiazine rearrangement.
    Journal of peptide science : an official publication of the European Peptide Society, 2021, Volume: 27, Issue:7

    Topics: Cysteine; Hydrogen-Ion Concentration; Maleimides; Molecular Structure; Peptides; Thiazines

2021
A Facile Procedure for One-Pot Stable Conjugation of Two Proglucagon Cysteine-Containing Peptide Analogs.
    Frontiers in endocrinology, 2021, Volume: 12

    Topics: Amino Acid Sequence; Animals; Cells, Cultured; Cricetinae; Cysteine; Glucagon-Like Peptide-1 Receptor; Humans; Maleimides; Organic Chemistry Phenomena; Peptides; Polymers; Proglucagon

2021
Rapid GSH detection and versatile peptide/protein labelling to track cell penetration using coumarin-based probes.
    The Analyst, 2023, Jan-31, Volume: 148, Issue:3

    Topics: Animals; Coumarins; Cysteine; Fluorescent Dyes; Glutathione; Homocysteine; Nucleosomes; Peptides; Sulfhydryl Compounds; Zebrafish

2023
Improving the stability of thiol-maleimide bioconjugates via the formation of a thiazine structure.
    Journal of peptide science : an official publication of the European Peptide Society, 2023, Volume: 29, Issue:10

    Topics: Cysteine; Glutathione; Maleimides; Peptides; Sulfhydryl Compounds

2023