maleic-acid and thiophenol

Metabolites regulate protein function via covalent and noncovalent interactions. However, manipulating these interactions in living cells remains a major challenge. Here, we report a chemical strategy for inducing cysteine S-succination, a nonenzymatic post-translational modification derived from the oncometabolite fumarate. Using a combination of antibody-based detection and kinetic assays, we benchmark the

Topics: Acylation; Cell Line, Tumor; Cysteine; Fumarates; HEK293 Cells; Humans; Hydrogen-Ion Concentration; Maleates; Phenols; Protein Processing, Post-Translational; Proteome; Proteomics; Succinates; Sulfhydryl Compounds