Page last updated: 2024-08-17

lysine and pyrrolysine

lysine has been researched along with pyrrolysine in 141 studies

Research

Studies (141)

Timeframe	Studies, this research(%)	All Research%
pre-1990	0 (0.00)	18.7374
1990's	0 (0.00)	18.2507
2000's	50 (35.46)	29.6817
2010's	82 (58.16)	24.3611
2020's	9 (6.38)	2.80

Authors

Authors	Studies
Atkins, JF; Gesteland, R	1
James, CM; Krzycki, JA; Srinivasan, G	1
Chan, MK; Ferguson, TK; Gong, W; Hao, B; James, CM; Krzycki, JA	1
Ibba, M; Söll, D	1
Fenske, C; Hinrichs, W; Palm, GJ	1
Ibba, M; Praetorius-Ibba, M	1
Ambrogelly, A; Ataide, SF; Ibba, M; Ishitani, R; Nureki, O; Polycarpo, C; Ruan, B; Söll, D; Tumbula-Hansen, D; Yokoyama, S	1
Brierley, I; Namy, O; Napthine, S; Rousset, JP	1
Frugier, M; Giegé, R; Rudinger-Thirion, J; Théobald-Dietrich, A	1
Ambrogelly, A; Bérubé, A; Crain, PF; McCloskey, JA; Polycarpo, C; Söll, D; Winbush, SM; Wood, JL	1
Blight, SK; Chan, MK; Chang, E; Green-Church, KB; Kang, PT; Krzycki, JA; Larue, RC; Longstaff, DG; Mahapatra, A; Zhao, G	1
Beebe, K; Schimmel, P	1
Chan, MK; Ferguson, TK; Gallucci, J; Hao, B; Kang, PT; Krzycki, JA; Soares, JA; Zhao, G	1
Krzycki, JA	3
Cobucci-Ponzano, B; Moracci, M; Rossi, M	2
Atkins, JF; Baranov, PV; Gladyshev, VN; Zhang, Y	1
Amster, J; Green-Church, KB; Jones, RB; Kleinholz, NM; Krzycki, JA; Pitsch, RL; Soares, JA; Wolff, JJ; Zhang, L	1
Chaudhuri, BN; Yeates, TO	1
Krzycki, JA; Larue, RC; Mahapatra, A; Metcalf, WW; Patel, A; Soares, JA; Zhang, JK	1
Gladyshev, VN; Hatfield, DL; Kryukov, GV; Lobanov, AV	1
Ambrogelly, A; Bérubé, A; Herring, S; Polycarpo, CR; Söll, D; Wood, JL	1
Blight, SK; Green-Church, KB; Krzycki, JA; Longstaff, DG; Zhang, L	1
Ambrogelly, A; Palioura, S; Söll, D	1
Faust, JE; Green-Church, KB; Krzycki, JA; Larue, RC; Longstaff, DG; Mahapatra, A; Zhang, L	1
Ambrogelly, A; Herring, S; Polycarpo, CR; Söll, D	1
Ambrogelly, A; Frauer, C; Gundllapalli, S; Herring, S; Polycarpo, C; Söll, D	1
Chan, M; Gottschalk, G; Kang, PT; Krzycki, JA; Lienard, T; Mahapatra, A; Metcalf, WW; Meyer, A; Richter, KB; Srinivasan, G; Zhang, JK; Zhao, G	1
Ambrogelly, A; Gundllapalli, S; Herring, S; O'Donoghue, P; Polycarpo, CR; Söll, D	1
Englert, M; Gundllapalli, S; Kavran, JM; O'Donoghue, P; Söll, D; Steitz, TA	1
Gladyshev, VN; Zhang, Y	1
Atkins, JF; Baranov, PV	1
Boucherie, H; Gomes, AC; Holstege, FC; Koerkamp, MJ; Lima-Costa, T; Manadas, B; Miranda, I; Moura, GR; Paredes, JA; Perrot, M; Rocha, R; Santos, MA; Silva, RM	1
Ambrogelly, A; Denise, A; Gundllapalli, S; Namy, O; Polycarpo, CR; Rousset, JP; Söll, D; Zhou, Y	1
Fukunaga, R; Ishii, R; Kobayashi, T; Sakamoto, K; Yanagisawa, T; Yokoyama, S	2
Hino, N; Kobayashi, T; Mukai, T; Sakamoto, K; Yanagisawa, T; Yokoyama, S	1
Chan, MK; Jain, R; Jiang, R; Krzycki, J; Larue, RC; Lee, MM	1
Ambrogelly, A; Gundllapalli, S; Li, D; Polycarpo, C; Söll, D; Umehara, T	1
Bechtel, J; Chan, MK; Kang, PT; Krzycki, JA; Li, WT; Longstaff, DG; Mahapatra, A; Zhao, G	1
Kobayashi, T; Sakamoto, K; Yanagisawa, T; Yokoyama, S	1
Araiso, Y; Gundllapalli, S; Ishitani, R; Nozawa, K; Nureki, O; O'Donoghue, P; Söll, D; Umehara, T	1
Chan, MK; Fekner, T; Lee, MM; Li, X	1
Cellitti, S; Chen, PR; Geierstanger, BH; Groff, D; Guo, J; Ou, W; Schultz, PG	1
Schwarzer, D	1
Dudev, T; Lim, C	1
Alkalaeva, E; Ambrogelly, A; Eliseev, B; Frolova, L; Gundllapalli, S; Kisselev, L; Kondrashov, FA; Söll, D; Vlasov, P	1
Chan, MK; Fekner, T; Li, X; Ottesen, JJ	1
Ambrogelly, A; Gundllapalli, S; O'Donoghue, P; Palioura, S; Sherrer, RL; Simonović, M; Söll, D; Yuan, J	1
Benner, J; Heinemann, IU; Madinger, C; Noren, CJ; O'Donoghue, P; Randau, L; Söll, D	1
Chan, MK; Fekner, T; Li, X	1
Lemke, EA	1
Ishitani, R; Nozawa, K; Nureki, O	1
Krzycki, JA; Rother, M	1
Lukashenko, NP	1
Nureki, O	1
Dodd, LE; Liu, WR; Pai, PJ; Russell, DH; Russell, WK; Wan, W; Wang, YS; Wang, Z	1
Chen, PR; Hao, Z; Liang, Y; Lin, S; Song, Y; Wang, J; Yang, M	1
Ragsdale, SW	1
Gaston, MA; Green-Church, KB; Krzycki, JA; Zhang, L	1
Chan, MK; Fekner, T	2
Anfora, AT; Brock, A; Cellitti, SE; Chiu, HP; Fan, Q; Geierstanger, BH; Grünewald, J; Hao, X; Jones, DH; Lesley, SA; Ng, K; Ou, W; Quinn, LL; Uno, T	1
Dellas, N; Noel, JP; Takimoto, JK; Wang, L	1
Gaston, MA; Jiang, R; Krzycki, JA	1
Chen, PR; Chen, X; Hao, Z; Hong, S	1
Bhattacharyya, M; Vishveshwara, S	1
Ai, HW; Chen, PR; Sagi, A; Schultz, PG; Shen, W	1
Chin, JW; Elliott, T; Kapadnis, PB; Lang, K; Madrzak, J; Nguyen, DP; Riechmann, L; Virdee, S	1
Chin, JW; Elliott, T; Holt, M; Muir, TW; Nguyen, DP	1
Hertweck, C	1
Bacher, A; Eisenreich, W; Groll, M; List, A; Quitterer, F	1
Hojo, H; Katayama, H; Nakahara, Y; Nozawa, K; Nureki, O	1
Dinman, JD	1
Guzei, IA; Kiessling, LL; Wong, ML	1
Chin, JW; Chou, C; Davis, L; Deiters, A; Lang, K; Torres-Kolbus, J	1
Jiang, R; Krzycki, JA	1
Bacher, A; Beck, P; Groll, M; List, A; Quitterer, F	1
Lin, Q; Pan, Y; Wang, J; Wang, Z; Yu, Z	1
Heinemann, IU; Ling, J; Liu, WR; O'Donoghue, P; Odoi, K; Prat, L; Söll, D	1
Chen, HY; Fang, X; Hilty, C; Liu, WR; Wang, YS; Wang, ZU; Wu, B	1
Carell, T; Gattner, MJ; Vrabel, M	1
Ishii, R; Sumida, T; Yanagisawa, T; Yokoyama, S	1
Das, G; Mandal, S	1
Das, G	2
Guo, Q; Huang, Y; Li, Y; Pan, M	1
Christiansen, H; Theil Have, C; Zambach, S	1
Au, SW; Chan, AH; Chan, MK; Fekner, T; Hsu, PH; Lee, MM; Tang, TH; Wang, L	1
An, WK; Han, MY; Jia, JY; Ma, BC; Wang, HZ; Wang, W; Zhang, Y	1
Bacher, A; Beck, P; Groll, M; Quitterer, F	2
Guo, LT; Ko, JH; Nakamura, A; Söll, D; Umehara, T; Wang, YS	1
Lacey, VK; Louie, GV; Noel, JP; Wang, L	1
Carell, T; Flügel, V; Gattner, MJ; López-Carrillo, V; Prill, S; Schneider, S; Vrabel, M	1
Chang, H; Chen, J; Chen, PR; Chen, R; Han, M; Huang, W; Wei, G; Xu, P; Xu, T; Zhang, J	1
Liu, WR; Tharp, JM; Wan, W	1
Borrel, G; Brugère, JF; Gaci, N; Gribaldo, S; O'Toole, PW; Peyret, P	1
Petersson, EJ; Warner, JB	1
Chin, JW; Cox, DJ; Lang, K; Mehl, RA; Sachdeva, A; Wallace, S; Wang, K; Wilf, NM	1
Behrman, EJ; Chan, MK; Fekner, T; Heater, BS; Hsu, PH; Lee, MM; Lu, J; Zhang, L	1
Meng, L; Wang, Z; Wu, W; Zhang, J; Zhou, M	1
Bardot, O; Borrel, G; Brugère, JF; Gaci, N; Gribaldo, S; Harris, HM; O'Toole, PW; Parisot, N; Peyret, P; Peyretaillade, E; Raymann, K; Tottey, W	1
Ferguson, DJ; Girosky, KE; Kountz, DJ; Krzycki, JA; Ticak, T	1
Heinemann, IU; Kucklick, M; O'Donoghue, P; Prat, L; Riedel, K; Rinehart, J; Schäfer, JG; Söll, D	1
Bretzler, J; Carell, T; Ehrlich, M; Eisen, D; Gattner, MJ; Stadlmeier, M; Viverge, B; Vrabel, M	1
Hohsaka, T; Kato, Y; Matsuura, T; Uyeda, A; Watanabe, H; Watanabe, T; Yomo, T	1
Güntert, P; Yilmaz, EM	1
Arbely, E; Cohen, S	1
Chern, J; Chou, CC; Kuo, CH; Lai, MC; Lai, SJ; Liang, SY; Wu, SH; Wu, WL; Yang, JT	1
Fiebig, J; Ludwig, MG; Lühmann, T; Meinel, L; Mueller, TD; Spieler, V; Werner, V	1
Liu, WR; Sharma, V; Wang, YS	1
Fasan, R; Grasso, KT; Owens, AE; Ziegler, CA	1
Alam, I; Ferry, JG; Guan, Y; Haroon, MF; Stingl, U	1
Brabham, R; Fascione, MA	1
Lopatniuk, M; Luzhetskyy, A; Myronovskyi, M	1
Cho, K; Park, HS; Yang, A	1
Bryson, DI; Guo, LT; Ho, JML; Liu, DR; Miller, C; Söll, D; Suzuki, T; Wang, YS	1
Böttke, T; Coin, I; Etzel, M; Lorenz, C; Mörl, M; Schicht, G; Serfling, R	1
Schmidt, MJ; Summerer, D	1
Chen, PR; Lin, S; Zheng, H	1
Ahern, CA; Galles, GD; Galpin, JD; Infield, DT; Lueck, JD	1
Bertozzi, CR; Gordon, CG; Han, Y; Sletten, EM; Tomlin, FM; Wu, TS	1
Aloush, N; Arbely, E; Ben-David, O; Brozgol, E; Cohen, S; Elia, N; Garini, Y; König, AI; Nachmias, D; Schvartz, T; Tam, B	1
Convertino, M; Deiters, A; Dokholyan, NV; Luo, J; Samanta, S	1
Lin, AE; Lin, Q	1
Budisa, N; Gerrits, M; Merk, H	1
Girona, GE; Lemke, EA; Reinkemeier, CD	1
Garcia, A; Kiefer, C; Marelli, M; Roy, G; Wilson, S	1
Kang, D; Lee, KJ; Park, HS	1
Seligmann, H; Warthi, G	1
Chen, Y; Ding, W; Lin, S; Wu, J; Yang, Y; Zang, J; Zhang, B; Zhao, H	1
Israsena, N; Jantarug, K; Sappakhaw, K; Slavoff, SA; Uttamapinant, C	1
Liu, WR; Rowlett, CM	1
Bennett, MR; Ho, JML; Mattia, JR; Miller, CA; Smith, KA	1
Budisa, N; Goettig, P; Koch, NG; Rappsilber, J	1
Geng, ZZ; Li, P; Liu, WR; Qiao, Y; Vatansever, EC; Xu, S; Yang, KS	1
Des Soye, BJ; Jewett, MC; Kelleher, NL; Ntai, I; Ranji Charna, A	1
Amikura, K; Fu, X; Guo, LT; Jiang, HK; Mukai, T; O'Donoghue, P; Söll, D; Tharp, JM; Wang, YS	1

Reviews

21 review(s) available for lysine and pyrrolysine

Article	Year
Genetic code: introducing pyrrolysine. Current biology : CB, 2002, Jul-09, Volume: 12, Issue:13 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Codon; Genes, Archaeal; Lysine; Methanosarcina barkeri; Methyltransferases; Protein Biosynthesis	2002
Aminoacyl-tRNA synthesis in archaea: different but not unique. Molecular microbiology, 2003, Volume: 48, Issue:3 Topics: Archaea; Codon; Lysine; Lysine-tRNA Ligase; RNA, Transfer, Amino Acyl; Selenocysteine; Transferases	2003
Reprogrammed genetic decoding in cellular gene expression. Molecular cell, 2004, Jan-30, Volume: 13, Issue:2 Topics: Animals; Base Sequence; Codon; Escherichia coli; Gene Expression; Humans; Lysine; Molecular Sequence Data; Protein Biosynthesis; RNA, Messenger; Saccharomyces cerevisiae; Selenocysteine	2004
Function of genetically encoded pyrrolysine in corrinoid-dependent methylamine methyltransferases. Current opinion in chemical biology, 2004, Volume: 8, Issue:5 Topics: Amides; Codon; Corrinoids; Dimethylamines; Imines; Lysine; Methane; Methylamines; Methyltransferases; Models, Molecular; Pyrroles	2004
Recoding in archaea. Molecular microbiology, 2005, Volume: 55, Issue:2 Topics: Archaea; Codon, Terminator; Frameshifting, Ribosomal; Lysine; Protein Biosynthesis; Selenocysteine	2005
The direct genetic encoding of pyrrolysine. Current opinion in microbiology, 2005, Volume: 8, Issue:6 Topics: Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Archaeal Proteins; Base Sequence; Codon; Genetic Code; Lysine; Methanosarcinaceae; Methylamines; Molecular Sequence Data; RNA, Transfer, Amino Acid-Specific	2005
Natural expansion of the genetic code. Nature chemical biology, 2007, Volume: 3, Issue:1 Topics: Amino Acyl-tRNA Synthetases; Archaea; Eukaryotic Cells; Evolution, Molecular; Genetic Code; Lysine; Molecular Structure; RNA; Selenocysteine	2007
Distinct genetic code expansion strategies for selenocysteine and pyrrolysine are reflected in different aminoacyl-tRNA formation systems. FEBS letters, 2010, Jan-21, Volume: 584, Issue:2 Topics: Codon, Terminator; Genetic Code; Lysine; RNA, Transfer, Amino Acyl; Selenocysteine; Transfer RNA Aminoacylation	2010
[Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality]. Seikagaku. The Journal of Japanese Biochemical Society, 2010, Volume: 82, Issue:7 Topics: Amino Acyl-tRNA Synthetases; Crystallography, X-Ray; Lysine; Multiprotein Complexes; Protein Biosynthesis; RNA, Transfer	2010
Selenocysteine, pyrrolysine, and the unique energy metabolism of methanogenic archaea. Archaea (Vancouver, B.C.), 2010, Aug-17, Volume: 2010 Topics: Archaeal Proteins; Codon, Terminator; Energy Metabolism; Euryarchaeota; Lysine; Methane; Phylogeny; Selenocysteine	2010
[Expanding genetic code: amino acids 21 and 22--selenocysteine and pyrrolysine]. Genetika, 2010, Volume: 46, Issue:8 Topics: Animals; Archaea; Bacteria; Codon, Terminator; Genetic Code; Humans; Lysine; Protein Biosynthesis; Selenocysteine	2010
[Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality]. Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme, 2009, Volume: 54, Issue:12 Suppl Topics: Amino Acyl-tRNA Synthetases; Codon, Terminator; Crystallography, X-Ray; Evolution, Molecular; Genetic Code; Lysine; Protein Biosynthesis; RNA, Transfer	2009
The pyrrolysine translational machinery as a genetic-code expansion tool. Current opinion in chemical biology, 2011, Volume: 15, Issue:3 Topics: Amino Acyl-tRNA Synthetases; Codon, Terminator; Escherichia coli; Eukaryota; Genetic Code; Lysine; Methanosarcina barkeri; Protein Engineering; Recombinant Proteins	2011
Functional context, biosynthesis, and genetic encoding of pyrrolysine. Current opinion in microbiology, 2011, Volume: 14, Issue:3 Topics: Archaea; Bacteria; Biosynthetic Pathways; Codon, Terminator; Gene Order; Humans; Lysine; Models, Biological; Models, Molecular; Protein Biosynthesis	2011
Control of gene expression by translational recoding. Advances in protein chemistry and structural biology, 2012, Volume: 86 Topics: Animals; Gene Expression Regulation; Humans; Lysine; Protein Biosynthesis; Ribosomes; Selenocysteine	2012
Translational recoding in archaea. Extremophiles : life under extreme conditions, 2012, Volume: 16, Issue:6 Topics: Archaea; Archaeal Proteins; Frameshifting, Ribosomal; Lysine; Selenocysteine	2012
The path of lysine to pyrrolysine. Current opinion in chemical biology, 2013, Volume: 17, Issue:4 Topics: Intramolecular Transferases; Ligases; Lysine; Methylation; Oxidation-Reduction	2013
Pyrrolysyl-tRNA synthetase: an ordinary enzyme but an outstanding genetic code expansion tool. Biochimica et biophysica acta, 2014, Volume: 1844, Issue:6 Topics: Amino Acyl-tRNA Synthetases; Codon, Terminator; Genetic Code; Genetic Engineering; Lysine; Methane; Methanosarcina; Methylamines; Models, Molecular; Protein Biosynthesis; RNA, Transfer; Substrate Specificity	2014
Pyrrolysine Amber Stop-Codon Suppression: Development and Applications. Chembiochem : a European journal of chemical biology, 2017, 10-18, Volume: 18, Issue:20 Topics: Amino Acyl-tRNA Synthetases; Click Chemistry; Codon, Terminator; Lysine; Protein Processing, Post-Translational	2017
Chemical biology approaches for studying posttranslational modifications. RNA biology, 2018, Volume: 15, Issue:4-5 Topics: Acetylation; Chemistry Techniques, Synthetic; Codon, Terminator; Genetic Code; Glycosylation; Humans; Lysine; Methylation; Nitrates; Phosphorylation; Protein Processing, Post-Translational; Proteome; Selenocysteine; Sulfates; Ubiquitination	2018
Site-Specific Labeling of Proteins Using Unnatural Amino Acids. Molecules and cells, 2019, May-31, Volume: 42, Issue:5 Topics: Click Chemistry; Fluorescent Dyes; Genetic Code; Lysine; Molecular Probes; Phosphoserine; Proteins; Selenocysteine	2019

Other Studies

120 other study(ies) available for lysine and pyrrolysine

Article	Year
Biochemistry. The 22nd amino acid. Science (New York, N.Y.), 2002, May-24, Volume: 296, Issue:5572 Topics: Animals; Anticodon; Codon; Codon, Terminator; Genetic Code; Lysine; Methanosarcina barkeri; Mitochondria; RNA; RNA, Archaeal; RNA, Mitochondrial; RNA, Transfer; RNA, Transfer, Amino Acyl; Selenocysteine	2002
Pyrrolysine encoded by UAG in Archaea: charging of a UAG-decoding specialized tRNA. Science (New York, N.Y.), 2002, May-24, Volume: 296, Issue:5572 Topics: Amino Acid Sequence; Amino Acyl-tRNA Synthetases; Anticodon; Archaeal Proteins; Base Sequence; Catalytic Domain; Codon; Codon, Terminator; Kinetics; Lysine; Methanosarcina barkeri; Methyltransferases; Molecular Sequence Data; Nucleic Acid Conformation; Protein Biosynthesis; Recombinant Proteins; RNA, Archaeal; RNA, Transfer; Sequence Alignment	2002
A new UAG-encoded residue in the structure of a methanogen methyltransferase. Science (New York, N.Y.), 2002, May-24, Volume: 296, Issue:5572 Topics: Archaeal Proteins; Bacterial Proteins; Codon; Crystallization; Crystallography, X-Ray; Dimerization; Genes, Archaeal; Hydrogen Bonding; Lysine; Methanosarcina barkeri; Methylamines; Methyltransferases; Models, Molecular; Molecular Weight; Protein Conformation; Protein Structure, Quaternary; Protein Structure, Secondary; Protein Structure, Tertiary; Spectrometry, Mass, Electrospray Ionization	2002
How unique is the genetic code? Angewandte Chemie (International ed. in English), 2003, Feb-10, Volume: 42, Issue:6 Topics: Amino Acids; Evolution, Molecular; Genetic Code; Genetic Variation; Lysine; Models, Molecular; RNA	2003
Activation of the pyrrolysine suppressor tRNA requires formation of a ternary complex with class I and class II lysyl-tRNA synthetases. Molecular cell, 2003, Volume: 12, Issue:2 Topics: Archaeal Proteins; Base Sequence; Codon, Terminator; DNA; Lysine; Lysine-tRNA Ligase; Methanosarcina barkeri; Methyltransferases; Models, Molecular; Molecular Sequence Data; Protein Binding; Protein Biosynthesis; RNA, Transfer; Time Factors; Transcription, Genetic	2003
Atypical archaeal tRNA pyrrolysine transcript behaves towards EF-Tu as a typical elongator tRNA. Nucleic acids research, 2004, Volume: 32, Issue:3 Topics: Anticodon; Base Sequence; Lysine; Lysine-tRNA Ligase; Methanosarcina barkeri; Mitochondria; Molecular Sequence Data; Nucleic Acid Conformation; Peptide Elongation Factor Tu; RNA, Archaeal; RNA, Transfer; RNA, Transfer, Ser; Selenocysteine; Yeasts	2004
An aminoacyl-tRNA synthetase that specifically activates pyrrolysine. Proceedings of the National Academy of Sciences of the United States of America, 2004, Aug-24, Volume: 101, Issue:34 Topics: Adenosine Monophosphate; Adenosine Triphosphate; Amino Acyl-tRNA Synthetases; Archaeal Proteins; Codon, Terminator; Lysine; Methanosarcina barkeri; Methyltransferases; Molecular Structure; Protein Biosynthesis	2004
Direct charging of tRNA(CUA) with pyrrolysine in vitro and in vivo. Nature, 2004, Sep-16, Volume: 431, Issue:7006 Topics: Acylation; Adenosine Triphosphate; Amino Acyl-tRNA Synthetases; Anticodon; Archaea; Archaeal Proteins; Cell-Free System; Codon; Diphosphates; Escherichia coli; Genetic Code; Lysine; Methyltransferases; RNA, Transfer, Amino Acid-Specific; Substrate Specificity; Suppression, Genetic	2004
Molecular biology: genetic code seizes pyrrolysine. Nature, 2004, Sep-16, Volume: 431, Issue:7006 Topics: Acylation; Amino Acyl-tRNA Synthetases; Archaea; Codon; Escherichia coli; Genetic Code; Lysine; RNA, Transfer, Amino Acid-Specific; Substrate Specificity	2004
Reactivity and chemical synthesis of L-pyrrolysine- the 22(nd) genetically encoded amino acid. Chemistry & biology, 2004, Volume: 11, Issue:9 Topics: Amino Acid Sequence; Archaeal Proteins; Crystallography, X-Ray; Dithionite; Hydroxylamine; Hydroxylamines; Lysine; Methanosarcina barkeri; Methyltransferases; Models, Molecular; Molecular Sequence Data; Molecular Structure	2004
Pyrrolysine and selenocysteine use dissimilar decoding strategies. The Journal of biological chemistry, 2005, May-27, Volume: 280, Issue:21 Topics: Amino Acid Sequence; Archaea; Archaeal Proteins; Codon; Codon, Terminator; Conserved Sequence; DNA Transposable Elements; Lysine; Methanosarcina; Methanosarcinaceae; Methylamines; Methyltransferases; Molecular Sequence Data; Operon; Phylogeny; Selenocysteine; Sequence Alignment	2005
The residue mass of L-pyrrolysine in three distinct methylamine methyltransferases. The Journal of biological chemistry, 2005, Nov-04, Volume: 280, Issue:44 Topics: Amides; Archaeal Proteins; Chromatography, Liquid; Chymotrypsin; Codon; Lysine; Methanosarcina barkeri; Methyltransferases; Peptide Fragments; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectroscopy, Fourier Transform Infrared	2005
A computational method to predict genetically encoded rare amino acids in proteins. Genome biology, 2005, Volume: 6, Issue:9 Topics: Amino Acid Sequence; Amino Acids; Archaea; Codon, Terminator; Computational Biology; DNA, Bacterial; Genetic Code; Genome, Bacterial; Lysine; Molecular Sequence Data; Nucleic Acid Conformation; Proteome; Selenoproteins; Sequence Alignment	2005
Characterization of a Methanosarcina acetivorans mutant unable to translate UAG as pyrrolysine. Molecular microbiology, 2006, Volume: 59, Issue:1 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Codon; Lysine; Methanosarcina; Methyltransferases; Multigene Family; Open Reading Frames; Protein Biosynthesis; RNA, Transfer	2006
Is there a twenty third amino acid in the genetic code? Trends in genetics : TIG, 2006, Volume: 22, Issue:7 Topics: Amino Acids; Codon, Terminator; Computational Biology; Genetic Code; Genome, Archaeal; Genome, Bacterial; Lysine; Nucleic Acid Conformation; RNA, Transfer; Selenocysteine	2006
Pyrrolysine analogues as substrates for pyrrolysyl-tRNA synthetase. FEBS letters, 2006, Dec-11, Volume: 580, Issue:28-29 Topics: Amino Acyl-tRNA Synthetases; Bacterial Proteins; beta-Galactosidase; Escherichia coli; Lysine; RNA, Messenger; Substrate Specificity; Time Factors; Tryptophan Synthase	2006
In vivo contextual requirements for UAG translation as pyrrolysine. Molecular microbiology, 2007, Volume: 63, Issue:1 Topics: Codon; Codon, Terminator; Lysine; Methanosarcina; Protein Biosynthesis	2007
A natural genetic code expansion cassette enables transmissible biosynthesis and genetic encoding of pyrrolysine. Proceedings of the National Academy of Sciences of the United States of America, 2007, Jan-16, Volume: 104, Issue:3 Topics: Amino Acid Sequence; Codon, Terminator; Escherichia coli; Gene Expression; Genetic Code; Genetic Vectors; Lysine; Mass Spectrometry; Methanosarcina; Methyltransferases; Molecular Sequence Data; Protein Biosynthesis; Time Factors	2007
Recognition of pyrrolysine tRNA by the Desulfitobacterium hafniense pyrrolysyl-tRNA synthetase. Nucleic acids research, 2007, Volume: 35, Issue:4 Topics: Amino Acyl-tRNA Synthetases; Aminoacylation; Base Sequence; Desulfitobacterium; Iodine; Lysine; Molecular Sequence Data; Protein Footprinting; Ribonucleases; RNA, Transfer	2007
Pyrrolysine is not hardwired for cotranslational insertion at UAG codons. Proceedings of the National Academy of Sciences of the United States of America, 2007, Feb-27, Volume: 104, Issue:9 Topics: Amino Acyl-tRNA Synthetases; Base Pairing; Base Sequence; Codon; Escherichia coli; Lysine; Methanosarcina barkeri; Molecular Sequence Data; Mutation; Protein Biosynthesis; RNA, Transfer, Amino Acid-Specific; Sequence Analysis, DNA; Suppression, Genetic; Transfer RNA Aminoacylation; Two-Hybrid System Techniques	2007
Class I and class II lysyl-tRNA synthetase mutants and the genetic encoding of pyrrolysine in Methanosarcina spp. Molecular microbiology, 2007, Volume: 64, Issue:5 Topics: Chemoautotrophic Growth; Lysine; Lysine-tRNA Ligase; Methanosarcina; Mutation	2007
The amino-terminal domain of pyrrolysyl-tRNA synthetase is dispensable in vitro but required for in vivo activity. FEBS letters, 2007, Jul-10, Volume: 581, Issue:17 Topics: Amino Acyl-tRNA Synthetases; Base Sequence; Desulfitobacterium; Enzyme Activation; Genetic Variation; Lysine; Methanosarcina; Molecular Sequence Data; Nucleic Acid Conformation; Protein Structure, Tertiary; Sequence Homology, Nucleic Acid; Substrate Specificity	2007
Structure of pyrrolysyl-tRNA synthetase, an archaeal enzyme for genetic code innovation. Proceedings of the National Academy of Sciences of the United States of America, 2007, Jul-03, Volume: 104, Issue:27 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Crystallography, X-Ray; Evolution, Molecular; Genetic Code; Hydrophobic and Hydrophilic Interactions; Lysine; Methanosarcina; Substrate Specificity	2007
High content of proteins containing 21st and 22nd amino acids, selenocysteine and pyrrolysine, in a symbiotic deltaproteobacterium of gutless worm Olavius algarvensis. Nucleic acids research, 2007, Volume: 35, Issue:15 Topics: Algorithms; Amino Acid Sequence; Animals; Bacterial Proteins; Deltaproteobacteria; Genome, Bacterial; Lysine; Molecular Sequence Data; Oligochaeta; Proteomics; Selenocysteine; Selenoproteins; Sequence Alignment; Symbiosis	2007
Translation: duality in the genetic code. Nature, 2007, Aug-30, Volume: 448, Issue:7157 Topics: Animals; Bacteria; Codon, Terminator; Genetic Code; Humans; Lysine; Protein Biosynthesis; Ribosomes; Selenocysteine	2007
Critical roles for a genetic code alteration in the evolution of the genus Candida. The EMBO journal, 2007, Oct-31, Volume: 26, Issue:21 Topics: Base Sequence; Candida; Genes, Fungal; Genetic Code; Genetic Techniques; Genome, Fungal; Genomics; Lysine; Molecular Sequence Data; Mutation; Nucleic Acid Conformation; Oligonucleotide Array Sequence Analysis; Proteomics; Saccharomyces cerevisiae; Selenocysteine	2007
Adding pyrrolysine to the Escherichia coli genetic code. FEBS letters, 2007, Nov-13, Volume: 581, Issue:27 Topics: Archaeal Proteins; Base Sequence; DNA Primers; DNA, Bacterial; Escherichia coli; Genes, Archaeal; Genes, Bacterial; Genetic Code; Lysine; Methanosarcina barkeri; Methyltransferases; Molecular Sequence Data; Nucleic Acid Conformation; Ornithine; RNA, Bacterial; Sequence Homology, Nucleic Acid	2007
Crystallographic studies on multiple conformational states of active-site loops in pyrrolysyl-tRNA synthetase. Journal of molecular biology, 2008, May-02, Volume: 378, Issue:3 Topics: Amino Acyl-tRNA Synthetases; Apoproteins; Base Sequence; Binding Sites; Crystallography, X-Ray; Lysine; Methanosarcina; Molecular Sequence Data; Mutagenesis, Site-Directed; Protein Structure, Tertiary; RNA, Transfer; Structure-Activity Relationship; Transfer RNA Aminoacylation	2008
Adding l-lysine derivatives to the genetic code of mammalian cells with engineered pyrrolysyl-tRNA synthetases. Biochemical and biophysical research communications, 2008, Jul-11, Volume: 371, Issue:4 Topics: Animals; Archaeal Proteins; CHO Cells; Cricetinae; Cricetulus; Epstein-Barr Virus Nuclear Antigens; Genetic Code; Humans; Lysine; Lysine-tRNA Ligase; Methanosarcina; Protein Biosynthesis; Protein Engineering	2008
Structure of Desulfitobacterium hafniense PylSc, a pyrrolysyl-tRNA synthetase. Biochemical and biophysical research communications, 2008, Sep-26, Volume: 374, Issue:3 Topics: Amino Acid Sequence; Bacterial Proteins; Crystallography, X-Ray; Desulfitobacterium; Hydrophobic and Hydrophilic Interactions; Lysine; Lysine-tRNA Ligase; Methanosarcina; Molecular Sequence Data; Protein Structure, Secondary	2008
Misacylation of pyrrolysine tRNA in vitro and in vivo. FEBS letters, 2008, Oct-15, Volume: 582, Issue:23-24 Topics: Alanine; Alanine-tRNA Ligase; Archaeal Proteins; Escherichia coli; Lysine; Methanosarcina barkeri; Methyltransferases; Mutation; RNA, Archaeal; RNA, Transfer, Lys; Serine; Serine-tRNA Ligase; Transfer RNA Aminoacylation	2008
Multistep engineering of pyrrolysyl-tRNA synthetase to genetically encode N(epsilon)-(o-azidobenzyloxycarbonyl) lysine for site-specific protein modification. Chemistry & biology, 2008, Nov-24, Volume: 15, Issue:11 Topics: Adenosine Triphosphate; Amino Acid Sequence; Aminoacylation; Azides; Catalytic Domain; Crystallography, X-Ray; Esterification; Fluorescent Dyes; Genes, Suppressor; Lysine; Lysine-tRNA Ligase; Methanosarcina; Mutagenesis, Site-Directed; Mutant Proteins; Point Mutation; RNA, Transfer	2008
Specificity of pyrrolysyl-tRNA synthetase for pyrrolysine and pyrrolysine analogs. Journal of molecular biology, 2009, Jan-30, Volume: 385, Issue:4 Topics: Adenosine Triphosphate; Amino Acyl-tRNA Synthetases; Caproates; Codon, Terminator; Escherichia coli; Kinetics; Lysine; Methanosarcina barkeri; Substrate Specificity; Suppression, Genetic; Transfer RNA Aminoacylation	2009
Recognition of non-alpha-amino substrates by pyrrolysyl-tRNA synthetase. Journal of molecular biology, 2009, Feb-06, Volume: 385, Issue:5 Topics: Amino Acyl-tRNA Synthetases; Binding Sites; Lysine; Methanosarcina; Models, Molecular; Substrate Specificity; Transfer RNA Aminoacylation	2009
Pyrrolysyl-tRNA synthetase-tRNA(Pyl) structure reveals the molecular basis of orthogonality. Nature, 2009, Feb-26, Volume: 457, Issue:7233 Topics: Amino Acyl-tRNA Synthetases; Aminoacylation; Crystallography, X-Ray; Desulfitobacterium; Escherichia coli; Lysine; Methanosarcina barkeri; Models, Molecular; RNA, Transfer, Amino Acid-Specific; Structural Homology, Protein	2009
A pyrrolysine analogue for protein click chemistry. Angewandte Chemie (International ed. in English), 2009, Volume: 48, Issue:9 Topics: Alkynes; Azides; Calmodulin; Catalysis; Codon, Terminator; Copper; Fluorescence Resonance Energy Transfer; Lysine; Recombinant Proteins	2009
A facile system for encoding unnatural amino acids in mammalian cells. Angewandte Chemie (International ed. in English), 2009, Volume: 48, Issue:22 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Animals; Archaea; Cell Line; CHO Cells; Cricetinae; Cricetulus; Escherichia coli; Genetic Code; Humans; Lysine; Protein Biosynthesis; Proteins	2009
Hacking the genetic code of mammalian cells. Chembiochem : a European journal of chemical biology, 2009, Jul-06, Volume: 10, Issue:10 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Cell Line; Escherichia coli; Genetic Code; Humans; Lysine	2009
Metal-binding affinity and selectivity of nonstandard natural amino acid residues from DFT/CDM calculations. The journal of physical chemistry. B, 2009, Aug-27, Volume: 113, Issue:34 Topics: 1-Carboxyglutamic Acid; Binding Sites; Computer Simulation; Lysine; Metals; Models, Chemical; Selenocysteine; Static Electricity	2009
Translation termination in pyrrolysine-utilizing archaea. FEBS letters, 2009, Nov-03, Volume: 583, Issue:21 Topics: Amino Acid Sequence; Archaeal Proteins; Codon, Terminator; Genome, Archaeal; Humans; Lysine; Methanosarcinaceae; Molecular Sequence Data; Peptide Termination Factors; Phylogeny; Protein Biosynthesis	2009
A pyrrolysine analogue for site-specific protein ubiquitination. Angewandte Chemie (International ed. in English), 2009, Volume: 48, Issue:48 Topics: Animals; Calmodulin; Humans; Lysine; Molecular Structure; Rats; Ubiquitination	2009
The appearance of pyrrolysine in tRNAHis guanylyltransferase by neutral evolution. Proceedings of the National Academy of Sciences of the United States of America, 2009, Dec-15, Volume: 106, Issue:50 Topics: Biological Evolution; Codon; Codon, Terminator; Lysine; Methanosarcina; Nucleotidyltransferases; Phylogeny; RNA, Messenger; RNA, Transfer, His	2009
N6-(2-(R)-propargylglycyl)lysine as a clickable pyrrolysine mimic. Chemistry, an Asian journal, 2010, Aug-02, Volume: 5, Issue:8 Topics: Alkynes; Azides; Calmodulin; Catalysis; Copper; Coumarins; Dipeptides; Fluorescent Dyes; Lysine; Stereoisomerism	2010
Precision control of cellular pathways with light. Chembiochem : a European journal of chemical biology, 2010, Sep-03, Volume: 11, Issue:13 Topics: Active Transport, Cell Nucleus; alpha Karyopherins; beta Karyopherins; Green Fluorescent Proteins; Light; Lysine; RNA, Transfer; Tumor Suppressor Protein p53	2010
The de novo engineering of pyrrolysyl-tRNA synthetase for genetic incorporation of L-phenylalanine and its derivatives. Molecular bioSystems, 2011, Volume: 7, Issue:3 Topics: Amino Acyl-tRNA Synthetases; Binding Sites; Codon, Nonsense; Escherichia coli Proteins; Lysine; Models, Molecular; Molecular Structure; Mutation; Phenylalanine; Protein Engineering; RNA, Transfer; Stereoisomerism	2011
A readily synthesized cyclic pyrrolysine analogue for site-specific protein "click" labeling. Chemical communications (Cambridge, England), 2011, Apr-21, Volume: 47, Issue:15 Topics: Binding Sites; Click Chemistry; Directed Molecular Evolution; Escherichia coli Proteins; HEK293 Cells; Humans; Lysine; Lysine-tRNA Ligase; Methanosarcina barkeri; Proteins; Staining and Labeling; Substrate Specificity; Tumor Suppressor Protein p53	2011
Biochemistry: How two amino acids become one. Nature, 2011, Mar-31, Volume: 471, Issue:7340 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Biocatalysis; Escherichia coli; Genetic Code; Lysine; Methanosarcina; Methyltransferases; Ornithine; RNA, Transfer, Amino Acid-Specific	2011
The complete biosynthesis of the genetically encoded amino acid pyrrolysine from lysine. Nature, 2011, Mar-31, Volume: 471, Issue:7340 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Biocatalysis; Escherichia coli; Genetic Code; Lysine; Mass Spectrometry; Methanosarcina; Methyltransferases; Molecular Structure; Ornithine; Peptides; Protein Biosynthesis; RNA, Transfer, Amino Acid-Specific; Transformation, Bacterial	2011
D-Ornithine coopts pyrrolysine biosynthesis to make and insert pyrroline-carboxy-lysine. Nature chemical biology, 2011, Apr-27, Volume: 7, Issue:8 Topics: Amino Acid Sequence; Escherichia coli; Gene Expression Regulation; HEK293 Cells; Humans; Lysine; Methanosarcina; Molecular Structure; Ornithine; Plasmids; Promoter Regions, Genetic	2011
Stereochemical basis for engineered pyrrolysyl-tRNA synthetase and the efficient in vivo incorporation of structurally divergent non-native amino acids. ACS chemical biology, 2011, Jul-15, Volume: 6, Issue:7 Topics: Amino Acid Sequence; Amino Acid Substitution; Amino Acyl-tRNA Synthetases; Aminoacylation; Catalytic Domain; Crystallography, X-Ray; Directed Molecular Evolution; Escherichia coli; HeLa Cells; Humans; Lysine; Methanosarcina; Methyltyrosines; Molecular Sequence Data; Mutation; Protein Conformation; Structure-Activity Relationship; Substrate Specificity	2011
Introducing bioorthogonal functionalities into proteins in living cells. Accounts of chemical research, 2011, Sep-20, Volume: 44, Issue:9 Topics: Alkynes; Amino Acids; Amino Acyl-tRNA Synthetases; Azides; Catalysis; Cell Line; Copper; Escherichia coli; Escherichia coli Proteins; Green Fluorescent Proteins; Humans; Ligases; Lysine; Proteins	2011
Probing the allosteric mechanism in pyrrolysyl-tRNA synthetase using energy-weighted network formalism. Biochemistry, 2011, Jul-19, Volume: 50, Issue:28 Topics: Allosteric Regulation; Amino Acyl-tRNA Synthetases; Bacterial Proteins; Catalytic Domain; Crystallography, X-Ray; Desulfitobacterium; Energy Metabolism; Ligands; Lysine; Models, Molecular; Molecular Dynamics Simulation; Protein Binding; Protein Folding; Protein Multimerization	2011
Probing protein-protein interactions with a genetically encoded photo-crosslinking amino acid. Chembiochem : a European journal of chemical biology, 2011, Aug-16, Volume: 12, Issue:12 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Cross-Linking Reagents; Cyclin-Dependent Kinase 5; Diazomethane; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Fatty Acids; HEK293 Cells; Humans; Lysine; Molecular Imaging; p21-Activated Kinases; Photochemical Processes; Plasmids; Protein Binding; Recombinant Proteins; RNA, Transfer; Signal Transduction; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Transfection; Transformation, Bacterial; Ultraviolet Rays	2011
Traceless and site-specific ubiquitination of recombinant proteins. Journal of the American Chemical Society, 2011, Jul-20, Volume: 133, Issue:28 Topics: Amino Acyl-tRNA Synthetases; Binding Sites; Lysine; Methanosarcina barkeri; Protein Engineering; Recombinant Proteins; Substrate Specificity; Ubiquitination	2011
Genetically encoded 1,2-aminothiols facilitate rapid and site-specific protein labeling via a bio-orthogonal cyanobenzothiazole condensation. Journal of the American Chemical Society, 2011, Aug-03, Volume: 133, Issue:30 Topics: Amino Acyl-tRNA Synthetases; Benzothiazoles; Binding Sites; Lysine; Methanosarcina; Models, Molecular; Molecular Conformation; Staining and Labeling; Substrate Specificity; Sulfhydryl Compounds	2011
Hidden in plain sight: the biosynthetic source of pyrrolysine revealed. Chembiochem : a European journal of chemical biology, 2011, Sep-19, Volume: 12, Issue:14 Topics: Genetic Engineering; Lysine	2011
Biosynthesis and charging of pyrrolysine, the 22nd genetically encoded amino acid. Angewandte Chemie (International ed. in English), 2011, Oct-04, Volume: 50, Issue:41 Topics: Lysine; Models, Molecular; Molecular Structure	2011
Crystal structure of methylornithine synthase (PylB): insights into the pyrrolysine biosynthesis. Angewandte Chemie (International ed. in English), 2012, Feb-06, Volume: 51, Issue:6 Topics: Crystallography, X-Ray; Iron-Sulfur Proteins; Ligases; Lysine; Methanosarcina barkeri; Models, Molecular; Ornithine; Protein Biosynthesis	2012
Pyrrolysine analogs as substrates for bacterial pyrrolysyl-tRNA synthetase in vitro and in vivo. Bioscience, biotechnology, and biochemistry, 2012, Volume: 76, Issue:1 Topics: Amino Acyl-tRNA Synthetases; Desulfitobacterium; Escherichia coli; Lysine; Protein Binding; Substrate Specificity	2012
An asymmetric synthesis of L-pyrrolysine. Organic letters, 2012, Mar-16, Volume: 14, Issue:6 Topics: Crystallography, X-Ray; Cyclization; Lysine; Molecular Conformation; Molecular Structure; Stereoisomerism	2012
Genetically encoded norbornene directs site-specific cellular protein labelling via a rapid bioorthogonal reaction. Nature chemistry, 2012, Feb-05, Volume: 4, Issue:4 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Cell Line; Fluorescent Dyes; Green Fluorescent Proteins; Humans; Lysine; Norbornanes; Proteins; Rhodamines	2012
PylSn and the homologous N-terminal domain of pyrrolysyl-tRNA synthetase bind the tRNA that is essential for the genetic encoding of pyrrolysine. The Journal of biological chemistry, 2012, Sep-21, Volume: 287, Issue:39 Topics: Amino Acyl-tRNA Synthetases; Anticodon; Archaeal Proteins; Bacterial Proteins; Desulfitobacterium; Lysine; Methanosarcina barkeri; Protein Structure, Secondary; Protein Structure, Tertiary; RNA, Archaeal; RNA, Bacterial; RNA, Transfer, Amino Acid-Specific; Substrate Specificity	2012
Biosynthesis of the 22nd genetically encoded amino acid pyrrolysine: structure and reaction mechanism of PylC at 1.5Å resolution. Journal of molecular biology, 2012, Dec-14, Volume: 424, Issue:5 Topics: Amino Acid Sequence; Biosynthetic Pathways; Catalytic Domain; Crystallography, X-Ray; Lysine; Methanosarcina barkeri; Models, Molecular; Molecular Sequence Data; Phosphotransferases; Protein Conformation	2012
Genetically encoded cyclopropene directs rapid, photoclick-chemistry-mediated protein labeling in mammalian cells. Angewandte Chemie (International ed. in English), 2012, Oct-15, Volume: 51, Issue:42 Topics: Amino Acid Sequence; Amino Acids; Amino Acyl-tRNA Synthetases; Animals; Archaeal Proteins; Click Chemistry; Cyclopropanes; Humans; Lysine; Mammals; Methanosarcina barkeri; Molecular Sequence Data; RNA, Transfer, Amino Acid-Specific	2012
Near-cognate suppression of amber, opal and quadruplet codons competes with aminoacyl-tRNAPyl for genetic code expansion. FEBS letters, 2012, Nov-02, Volume: 586, Issue:21 Topics: Anticodon; Codon; Escherichia coli; Genetic Code; Lysine; Protein Biosynthesis; RNA, Transfer, Amino Acyl; Spectrometry, Mass, Electrospray Ionization; Suppression, Genetic	2012
Genetic incorporation of twelve meta-substituted phenylalanine derivatives using a single pyrrolysyl-tRNA synthetase mutant. ACS chemical biology, 2013, Feb-15, Volume: 8, Issue:2 Topics: Amino Acyl-tRNA Synthetases; Escherichia coli; Lysine; Models, Molecular; Molecular Structure; Mutant Proteins; Mutation; Phenylalanine; Substrate Specificity	2013
Synthesis of ε-N-propionyl-, ε-N-butyryl-, and ε-N-crotonyl-lysine containing histone H3 using the pyrrolysine system. Chemical communications (Cambridge, England), 2013, Jan-14, Volume: 49, Issue:4 Topics: Amino Acids; Blotting, Western; Butanols; Histones; Lysine; Models, Molecular	2013
A novel crystal form of pyrrolysyl-tRNA synthetase reveals the pre- and post-aminoacyl-tRNA synthesis conformational states of the adenylate and aminoacyl moieties and an asparagine residue in the catalytic site. Acta crystallographica. Section D, Biological crystallography, 2013, Volume: 69, Issue:Pt 1 Topics: Amino Acid Motifs; Amino Acyl-tRNA Synthetases; Aminoacylation; Asparagine; Catalytic Domain; Crystallography, X-Ray; Hydrogen Bonding; Lysine; Methanosarcina; Protein Binding; Protein Conformation; Protein Multimerization; Substrate Specificity	2013
DFT studies on the intrinsic conformational properties of non-ionic pyrrolysine in gas phase. Journal of molecular modeling, 2013, Volume: 19, Issue:4 Topics: Archaea; Gases; Hydrogen Bonding; Kinetics; Lysine; Molecular Conformation; Quantum Theory; Spectrophotometry, Infrared; Thermodynamics; Vibration	2013
Investigations of dipeptide structures containing pyrrolysine as N-terminal residues: a DFT study in gas and aqueous phase. Journal of molecular modeling, 2013, Volume: 19, Issue:4 Topics: Archaea; Dipeptides; Gases; Hydrogen Bonding; Kinetics; Lysine; Molecular Conformation; Quantum Theory; Solutions; Spectrophotometry, Infrared; Thermodynamics; Vibration; Water	2013
Thiol-yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue. Organic & biomolecular chemistry, 2013, Apr-28, Volume: 11, Issue:16 Topics: Alkynes; Amino Acyl-tRNA Synthetases; Escherichia coli; Escherichia coli Proteins; Lysine; Models, Molecular; Mutation; Staining and Labeling; Sulfhydryl Compounds	2013
Effects of using coding potential, sequence conservation and mRNA structure conservation for predicting pyrrolysine containing genes. BMC bioinformatics, 2013, Apr-04, Volume: 14 Topics: Base Sequence; Cluster Analysis; Codon, Terminator; Conserved Sequence; Evolution, Molecular; Genome, Archaeal; Genome, Bacterial; Genomics; Lysine; Methyltransferases; Open Reading Frames; Protein Biosynthesis; RNA, Messenger; Sequence Alignment	2013
Zwitterionic conformers of pyrrolysine and their interactions with metal ions--a theoretical study. Journal of molecular modeling, 2013, Volume: 19, Issue:8 Topics: Archaea; Bacteria; Cations, Divalent; Copper; Gases; Hydrogen Bonding; Isomerism; Lysine; Models, Chemical; Molecular Conformation; Quantum Theory; Temperature; Thermodynamics; Water	2013
A click-and-release pyrrolysine analogue. Chembiochem : a European journal of chemical biology, 2013, May-10, Volume: 14, Issue:7 Topics: Click Chemistry; Humans; Lysine; Sumoylation; Thymine DNA Glycosylase	2013
A concise synthesis of L-pyrrolysine. Chemistry (Weinheim an der Bergstrasse, Germany), 2013, Jun-17, Volume: 19, Issue:25 Topics: Amides; Imines; Lysine; Molecular Structure; Stereoisomerism	2013
Structure and reaction mechanism of pyrrolysine synthase (PylD). Angewandte Chemie (International ed. in English), 2013, Jul-01, Volume: 52, Issue:27 Topics: Amino Acids; Lysine; Oxidoreductases; Structure-Activity Relationship	2013
Pyrrolysyl-tRNA synthetase variants reveal ancestral aminoacylation function. FEBS letters, 2013, Oct-01, Volume: 587, Issue:19 Topics: Amino Acyl-tRNA Synthetases; Aminoacylation; Codon; Escherichia coli; Evolution, Molecular; Lysine; Mutation; Substrate Specificity	2013
Expanding the library and substrate diversity of the pyrrolysyl-tRNA synthetase to incorporate unnatural amino acids containing conjugated rings. Chembiochem : a European journal of chemical biology, 2013, Nov-04, Volume: 14, Issue:16 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Animals; Directed Molecular Evolution; Escherichia coli; Gene Library; Genetic Code; Lysine; Myoglobin; Substrate Specificity; Whales	2013
Structural insights into incorporation of norbornene amino acids for click modification of proteins. Chembiochem : a European journal of chemical biology, 2013, Nov-04, Volume: 14, Issue:16 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Click Chemistry; Crystallography, X-Ray; Lysine; Methanosarcina; Mutation; Norbornanes; Protein Structure, Tertiary	2013
Light-induced protein translocation by genetically encoded unnatural amino acid in Caenorhabditis elegans. Protein & cell, 2013, Volume: 4, Issue:12 Topics: Animals; Caenorhabditis elegans; Caenorhabditis elegans Proteins; Light; Lysine; Promoter Regions, Genetic; Protein Transport; RNA, Transfer; Tumor Necrosis Factor Ligand Superfamily Member 14	2013
Unique characteristics of the pyrrolysine system in the 7th order of methanogens: implications for the evolution of a genetic code expansion cassette. Archaea (Vancouver, B.C.), 2014, Volume: 2014 Topics: Amino Acyl-tRNA Synthetases; Bacteria; Biosynthetic Pathways; Euryarchaeota; Evolution, Molecular; Gene Transfer, Horizontal; Genetic Code; Lysine; Phylogeny; RNA, Transfer; Sequence Homology	2014
Synthetic biology: Two-for-one designer labels. Nature chemistry, 2014, Volume: 6, Issue:5 Topics: Fluorescence Resonance Energy Transfer; Lysine; Proteins; Staining and Labeling	2014
Optimized orthogonal translation of unnatural amino acids enables spontaneous protein double-labelling and FRET. Nature chemistry, 2014, Volume: 6, Issue:5 Topics: Amino Acyl-tRNA Synthetases; Base Sequence; Codon; Fluorescence Resonance Energy Transfer; Lysine; Models, Molecular; Molecular Structure; Proteins; Ribosomes; RNA, Transfer; Staining and Labeling	2014
The formation of pyrroline and tetrahydropyridine rings in amino acids catalyzed by pyrrolysine synthase (PylD). Angewandte Chemie (International ed. in English), 2014, Jul-28, Volume: 53, Issue:31 Topics: Catalysis; Catalytic Domain; Ligases; Lysine; Pyridines; Pyrroles	2014
Pyrrolysine-inspired protein cyclization. Chembiochem : a European journal of chemical biology, 2014, Aug-18, Volume: 15, Issue:12 Topics: Cyclization; Humans; Lysine; MCF-7 Cells; Oligopeptides; Protein Conformation; Proteins	2014
Low energy conformations and gas-phase acidity and basicity of pyrrolysine. The journal of physical chemistry. A, 2014, Aug-28, Volume: 118, Issue:34 Topics: Computer Simulation; Feasibility Studies; Gases; Hydrogen Bonding; Lysine; Models, Molecular; Molecular Conformation; Protons; Rotation; Spectrophotometry, Infrared; Temperature; Vibration	2014
Comparative genomics highlights the unique biology of Methanomassiliicoccales, a Thermoplasmatales-related seventh order of methanogenic archaea that encodes pyrrolysine. BMC genomics, 2014, Aug-13, Volume: 15 Topics: Archaeal Proteins; Biosynthetic Pathways; Clustered Regularly Interspaced Short Palindromic Repeats; Codon, Terminator; Energy Metabolism; Genome, Archaeal; Lysine; Molecular Sequence Data; Phylogeny; Replication Origin; RNA, Archaeal; RNA, Ribosomal; Thermoplasmales	2014
A nonpyrrolysine member of the widely distributed trimethylamine methyltransferase family is a glycine betaine methyltransferase. Proceedings of the National Academy of Sciences of the United States of America, 2014, Oct-28, Volume: 111, Issue:43 Topics: Betaine; Chromatography, Thin Layer; Desulfitobacterium; Genes, Bacterial; Glycine N-Methyltransferase; Humans; Lysine; Methylamines; Methylation; Phylogeny; Real-Time Polymerase Chain Reaction	2014
Reducing the genetic code induces massive rearrangement of the proteome. Proceedings of the National Academy of Sciences of the United States of America, 2014, Dec-02, Volume: 111, Issue:48 Topics: Adaptation, Physiological; Archaeal Proteins; Chromatography, Liquid; Codon, Terminator; Electrophoresis, Gel, Two-Dimensional; Genetic Code; Lysine; Methanosarcina; Methylamines; Mutation; Protein Biosynthesis; Proteome; Proteomics; RNA, Transfer, Amino Acid-Specific; Tandem Mass Spectrometry	2014
Orchestrating the biosynthesis of an unnatural pyrrolysine amino Acid for its direct incorporation into proteins inside living cells. Chemistry (Weinheim an der Bergstrasse, Germany), 2015, May-18, Volume: 21, Issue:21 Topics: Amino Acid Sequence; Biosynthetic Pathways; Carbonic Anhydrases; Escherichia coli; Humans; Lysine; Models, Molecular; Protein Biosynthesis; Protein Engineering	2015
Liposome-Based in Vitro Evolution of Aminoacyl-tRNA Synthetase for Enhanced Pyrrolysine Derivative Incorporation. Chembiochem : a European journal of chemical biology, 2015, Aug-17, Volume: 16, Issue:12 Topics: Amino Acyl-tRNA Synthetases; Evolution, Molecular; Gene Library; Liposomes; Lysine; Models, Molecular; Sequence Analysis	2015
NMR structure calculation for all small molecule ligands and non-standard residues from the PDB Chemical Component Dictionary. Journal of biomolecular NMR, 2015, Volume: 63, Issue:1 Topics: ADAM Proteins; ADAM17 Protein; Amino Acids; Databases, Chemical; Databases, Protein; Enzyme Inhibitors; Ligands; Lysine; Nuclear Magnetic Resonance, Biomolecular; Oligopeptides; Small Molecule Libraries	2015
Single-Plasmid-Based System for Efficient Noncanonical Amino Acid Mutagenesis in Cultured Mammalian Cells. Chembiochem : a European journal of chemical biology, 2016, 06-02, Volume: 17, Issue:11 Topics: Acetylation; Amino Acids; Amino Acyl-tRNA Synthetases; Animals; Genetic Code; HCT116 Cells; HEK293 Cells; Humans; Lysine; Mice; Mutagenesis; NIH 3T3 Cells; Plasmids; Protein Engineering; Tumor Suppressor Protein p53	2016
Phosphoproteomic analysis of Methanohalophilus portucalensis FDF1(T) identified the role of protein phosphorylation in methanogenesis and osmoregulation. Scientific reports, 2016, 06-30, Volume: 6 Topics: Amino Acid Sequence; Archaeal Proteins; Betaine; Global Warming; Glycine N-Methyltransferase; Lysine; Methane; Methanosarcinaceae; Methylation; Models, Molecular; Mutation, Missense; Osmoregulation; Phosphoproteins; Phosphorylation; Point Mutation; Protein Conformation; Protein Processing, Post-Translational; Proteomics; Salt Tolerance; Tandem Mass Spectrometry	2016
Interleukin-4-Clicked Surfaces Drive M2 Macrophage Polarization. Chembiochem : a European journal of chemical biology, 2016, Nov-17, Volume: 17, Issue:22 Topics: Alkynes; Amino Acid Sequence; Amino Acids; Azides; Catalysis; Cell Polarity; Cells, Cultured; Circular Dichroism; Copper; Cycloaddition Reaction; Genetic Code; HEK293 Cells; Humans; Interferon-gamma; Interleukin-4; Lipopolysaccharides; Lysine; Macrophage Colony-Stimulating Factor; Macrophages; Molecular Sequence Data; Monocytes; Mutagenesis, Site-Directed; Sepharose; Surface Properties	2016
Probing the Catalytic Charge-Relay System in Alanine Racemase with Genetically Encoded Histidine Mimetics. ACS chemical biology, 2016, 12-16, Volume: 11, Issue:12 Topics: Alanine Racemase; Escherichia coli; Histidine; Hydrogen Bonding; Lysine; Models, Molecular; Mutagenesis, Site-Directed	2016
Two-Tier Screening Platform for Directed Evolution of Aminoacyl-tRNA Synthetases with Enhanced Stop Codon Suppression Efficiency. Chembiochem : a European journal of chemical biology, 2017, 06-19, Volume: 18, Issue:12 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Bacterial Proteins; Cloning, Molecular; Codon, Terminator; Directed Molecular Evolution; Escherichia coli; Genetic Code; High-Throughput Screening Assays; Lysine; Methanosarcina barkeri; Mutation; Protein Biosynthesis; Protein Engineering; Protein Processing, Post-Translational; Recombinant Proteins; Ribosomes	2017
Single-cell genomics reveals pyrrolysine-encoding potential in members of uncultivated archaeal candidate division MSBL1. Environmental microbiology reports, 2017, Volume: 9, Issue:4 Topics: Archaea; Archaeal Proteins; Codon, Terminator; Genome, Archaeal; Genomics; Indian Ocean; Lysine; Methyltransferases; Phylogeny	2017
Streptomyces albus: A New Cell Factory for Non-Canonical Amino Acids Incorporation into Ribosomally Synthesized Natural Products. ACS chemical biology, 2017, 09-15, Volume: 12, Issue:9 Topics: Amino Acyl-tRNA Synthetases; Bacteriocins; Biological Products; Cloning, Molecular; Industrial Microbiology; Lysine; Methanosarcina barkeri; Multigene Family; Peptides, Cyclic; Protein Biosynthesis; Ribosomes; Streptomyces	2017
Crystal structures reveal an elusive functional domain of pyrrolysyl-tRNA synthetase. Nature chemical biology, 2017, Volume: 13, Issue:12 Topics: Amino Acyl-tRNA Synthetases; Crystallography, X-Ray; Directed Molecular Evolution; Lysine; Methanosarcina; Models, Molecular	2017
Designer tRNAs for efficient incorporation of non-canonical amino acids by the pyrrolysine system in mammalian cells. Nucleic acids research, 2018, 01-09, Volume: 46, Issue:1 Topics: Amino Acyl-tRNA Synthetases; Base Sequence; Codon, Terminator; Escherichia coli; Genes, Reporter; HEK293 Cells; Humans; Luminescent Proteins; Lysine; Nucleic Acid Conformation; Protein Engineering; Receptors, Corticotropin-Releasing Hormone; RNA Processing, Post-Transcriptional; RNA, Transfer, Amino Acyl	2018
Directed Evolution of Orthogonal Pyrrolysyl-tRNA Synthetases in Escherichia coli for the Genetic Encoding of Noncanonical Amino Acids. Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1728 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Codon; Culture Media; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Evolution, Molecular; Fluorescent Antibody Technique; Gene Expression; Gene Order; Lysine; Plasmids	2018
Genetic Code Expansion in Enteric Bacterial Pathogens. Methods in molecular biology (Clifton, N.J.), 2018, Volume: 1728 Topics: Amino Acids; Bacterial Proteins; Enterobacteriaceae; Gene Expression; Genes, Reporter; Genetic Code; Humans; Lysine; Mass Spectrometry; Mutagenesis, Site-Directed; Plasmids; Promoter Regions, Genetic; Protein Engineering; RNA, Transfer	2018
Orthogonality of Pyrrolysine tRNA in the Xenopus oocyte. Scientific reports, 2018, 03-26, Volume: 8, Issue:1 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Aminoacylation; Animals; Anticodon; Codon, Terminator; Genetic Code; Humans; Lysine; Methanosarcina barkeri; Oocytes; Patch-Clamp Techniques; Protein Biosynthesis; Rats; RNA, Transfer; Tetrahymena thermophila; Transfer RNA Aminoacylation; Voltage-Gated Sodium Channels; Xenopus laevis	2018
Site-specific incorporation of quadricyclane into a protein and photocleavage of the quadricyclane ligation adduct. Bioorganic & medicinal chemistry, 2018, 10-15, Volume: 26, Issue:19 Topics: Biotin; Bridged-Ring Compounds; Coordination Complexes; Lysine; Nickel; Photolysis; Streptavidin; Ultraviolet Rays	2018
Live Cell Imaging of Bioorthogonally Labelled Proteins Generated With a Single Pyrrolysine tRNA Gene. Scientific reports, 2018, 09-28, Volume: 8, Issue:1 Topics: Animals; Chlorocebus aethiops; Codon, Terminator; COS Cells; Genetic Code; Lysine; Optical Imaging; Protein Biosynthesis; Proteins; RNA, Transfer; Transfection	2018
Reversible and Tunable Photoswitching of Protein Function through Genetic Encoding of Azobenzene Amino Acids in Mammalian Cells. Chembiochem : a European journal of chemical biology, 2018, 10-18, Volume: 19, Issue:20 Topics: Amino Acyl-tRNA Synthetases; Azo Compounds; HEK293 Cells; Humans; Isomerism; Kinetics; Light; Luciferases; Lysine; Phenylalanine; RNA, Transfer	2018
Rapid Identification of Functional Pyrrolysyl-tRNA Synthetases via Fluorescence-Activated Cell Sorting. International journal of molecular sciences, 2018, Dec-21, Volume: 20, Issue:1 Topics: Amino Acids; Amino Acyl-tRNA Synthetases; Flow Cytometry; Gene Library; Genetic Code; Lysine; Substrate Specificity	2018
Site-Specific Chemoselective Pyrrolysine Analogues Incorporation Using the Cell-Free Protein Synthesis System. ACS synthetic biology, 2019, 02-15, Volume: 8, Issue:2 Topics: Amino Acyl-tRNA Synthetases; Cell-Free System; Lysine; Protein Biosynthesis; Protein Engineering	2019
Designer membraneless organelles enable codon reassignment of selected mRNAs in eukaryotes. Science (New York, N.Y.), 2019, 03-29, Volume: 363, Issue:6434 Topics: Animals; Caenorhabditis elegans; Cell Membrane; Chlorocebus aethiops; Codon; COS Cells; Genetic Code; HEK293 Cells; Humans; Lysine; Methanosarcina; Organelles; Protein Biosynthesis; Protein Engineering; Ribosomes; RNA, Messenger; RNA, Transfer; Synthetic Biology	2019
qPCR assays to quantitate tRNApyl and pylRS expression in engineered cell lines. PloS one, 2019, Volume: 14, Issue:5 Topics: Amino Acyl-tRNA Synthetases; Animals; Bacterial Proteins; CHO Cells; Cricetulus; Genetic Engineering; Lysine; Methanosarcina; Real-Time Polymerase Chain Reaction; RNA, Transfer	2019
Natural pyrrolysine-biased translation of stop codons in mitochondrial peptides entirely coded by expanded codons. Bio Systems, 2020, Volume: 196 Topics: Codon, Terminator; Humans; Lysine; Mitochondria; Peptide Fragments; Protein Biosynthesis; RNA, Transfer	2020
Chimeric design of pyrrolysyl-tRNA synthetase/tRNA pairs and canonical synthetase/tRNA pairs for genetic code expansion. Nature communications, 2020, 06-22, Volume: 11, Issue:1 Topics: Alanine; Amino Acids; Chimera; Escherichia coli; Genetic Code; HEK293 Cells; Histidine; Humans; Lysine; Phenylalanine; RNA, Transfer; Synthetic Biology; Tryptophan; Tyrosine	2020
A Genetic Code Expansion-Derived Molecular Beacon for the Detection of Intracellular Amyloid-β Peptide Generation. Angewandte Chemie (International ed. in English), 2021, 02-19, Volume: 60, Issue:8 Topics: Amino Acyl-tRNA Synthetases; Amyloid beta-Peptides; Amyloid beta-Protein Precursor; Archaeal Proteins; Genetic Code; HEK293 Cells; Humans; Lysine; Methanosarcina; Microscopy, Fluorescence; Mutagenesis, Site-Directed; Protein Processing, Post-Translational	2021
Site Specific Lysine Acetylation of Histones for Nucleosome Reconstitution using Genetic Code Expansion in Escherichia coli. Journal of visualized experiments : JoVE, 2020, 12-26, Issue:166 Topics: Acetylation; Amino Acyl-tRNA Synthetases; DNA; Endopeptidases; Escherichia coli; Escherichia coli Proteins; Genetic Code; Histones; Lysine; Methanosarcina; Nucleosomes; Plasmids; Protein Multimerization; Protein Processing, Post-Translational; RNA, Transfer	2020
Improved pyrrolysine biosynthesis through phage assisted non-continuous directed evolution of the complete pathway. Nature communications, 2021, 06-24, Volume: 12, Issue:1 Topics: Bacterial Proteins; Biosynthetic Pathways; Coliphages; Directed Molecular Evolution; Escherichia coli; Green Fluorescent Proteins; Lysine; Microorganisms, Genetically-Modified; Mutation; Operon; Recombinant Proteins; RNA, Transfer; Solubility	2021
Engineering Pyrrolysyl-tRNA Synthetase for the Incorporation of Non-Canonical Amino Acids with Smaller Side Chains. International journal of molecular sciences, 2021, Oct-17, Volume: 22, Issue:20 Topics: Amino Acyl-tRNA Synthetases; Archaeal Proteins; Genetic Code; Lysine; Methanosarcina barkeri; Protein Engineering; Substrate Specificity	2021
A Designed, Highly Efficient Pyrrolysyl-tRNA Synthetase Mutant Binds o-Chlorophenylalanine Using Two Halogen Bonds. Journal of molecular biology, 2022, 04-30, Volume: 434, Issue:8 Topics: Amino Acyl-tRNA Synthetases; Genetic Code; Halogens; Lysine; Methanosarcina; Mutation; Phenylalanine; Protein Binding	2022
An efficient cell-free protein synthesis platform for producing proteins with pyrrolysine-based noncanonical amino acids. Biotechnology journal, 2022, Volume: 17, Issue:9 Topics: Amino Acids; Amino Acids, Basic; Escherichia coli; Green Fluorescent Proteins; Lysine; Protein Biosynthesis; RNA, Transfer	2022
Ancestral archaea expanded the genetic code with pyrrolysine. The Journal of biological chemistry, 2022, Volume: 298, Issue:11 Topics: Amino Acyl-tRNA Synthetases; Archaea; Genetic Code; Lysine; Methanosarcina; RNA, Transfer	2022