lusianthridin and 7-hydroxy-2-methoxy-1-4-phenanthrenedione

Chemical investigation and activity test of Arundina graminifolia led to the isolation of six phenanthrenes: blestriarene A (1), shancidin (2), densiflorol B (3), ephemeranthoquinone (4), coelonin (5) and lusianthridin (6). The isolated compounds demonstrated antibacterial and anti-haemolytic activities. It was found that compounds 1 and 2 had medium antibacterial activity against Staphylococcus aureus, Bacillus subtilis and Escherichia coli, with MICs of 20-40 μg/mL and MBCs of 40-320 μg/mL. Bactericidal mechanisms were explored. Rupture of cell wall and membrane and leakage of nuclear mass were observed by transmission electron microscopy (TEM). Moreover, compounds 1-3 attenuated the erythrocyte damage. Compounds 1 and 2 showed significant anti-haemolytic activity with inhibition rate about 50% at 16 μg/mL.

Topics: Animals; Anti-Bacterial Agents; Bacillus subtilis; Drug Evaluation, Preclinical; Erythrocytes; Escherichia coli; Hemolysis; Microbial Sensitivity Tests; Microscopy, Electron, Transmission; Molecular Structure; Orchidaceae; Phenanthrenes; Rabbits; Staphylococcus aureus

To isolate and elucidate the constituents from rhizome of Arundina graminifolia.. Theconstituents were extracted with 95% alcohol and isolated by chromatography on silica gel, Sephedax LH-20. The structures were determined by UV, IR, NMR and MS spectral analysis.. Five phenanthrene constituents were identified as 7-hydroxy-2, 4-dimethoxy-9, 10-dihydrophenanthrene( I ), 4, 7-dihydroxy-2-methoxy-9, 10-dihydrophenanthrene ( II ), 2, 7-dihydroxy-4-methoxy-9, 10-dihydrophenanthrene ( III ), 7-hydroxy-2-methoxyphenanthrene-1,4-dione ( IV ), 7-hydroxy-2-methoxy-9, 10-dihydrophenanthrene-1,4-dione (V), respectively.. All compounds were isolated from rhizome of A. graminifolia for the first time.

Topics: Orchidaceae; Phenanthrenes; Plants, Medicinal; Rhizome