lucifer-yellow and mezerein

The effect of the tumor promoters 12-O-tetradecanoylphorbol-13-acetate (TPA), mezerein, teleocidin, anthralin, the Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), dichlorodiphenyltrichloroethane (DDT) and phenobarbital (PB) on lucifer yellow transfer in cultures of SV-40-transformed Djungarian hamster fibroblasts was studied. TPA, mezerein, teleocidin, A23187, DDT and BHT exerted a strong inhibitory effect on cell-to-cell dye transfer. Anthralin uncoupled cells in 3 experiments out of 6. PB appeared to enhance lucifer yellow transfer. Sodium nitrite, a substance with unknown promoting activity, effectively uncoupled cells. All the promoters investigated had a reversible effect on the dye transfer. The value of the dye transfer method for promoter screening is discussed.

Topics: Animals; Anthralin; Butylated Hydroxytoluene; Calcimycin; Carcinogens; Cells, Cultured; Cricetinae; DDT; Diterpenes; Fluorescent Dyes; Intercellular Junctions; Isoquinolines; Lyngbya Toxins; Phenobarbital; Terpenes; Tetradecanoylphorbol Acetate

The effects of tumor promoter--12-0-tetradecanoylphorbol-13-acetate (TPA), mezerein, anthralin, Ca2+-ionophore A23187, butylated hydroxytoluene (BHT), DDT and phenobarbital--on cell-to-cell exchange of Lucifer Yellow were studied in cultures of SV40-transformed Djungarian hamster fibroblasts. TPA, mezerein, A23187, DDT and BHT strongly inhibited cell-to-cell exchange of Lucifer Yellow. Anthralin uncoupled cells in 3 out of 6 experiments. Phenobarbital, in contrast to other promoters, enhanced dye transfer. The effects of all the promoters tested were fully reversible. The potential use of Lucifer Yellow exchange inhibition as a test for the screening of tumor promoters is discussed.

Topics: Animals; Anthralin; Butylated Hydroxytoluene; Calcimycin; Carcinogens; Cell Line; Cricetinae; DDT; Diterpenes; Fluorescent Dyes; In Vitro Techniques; Isoquinolines; Phenobarbital; Terpenes; Tetradecanoylphorbol Acetate