lucifer-yellow and irsogladine

The effects of drugs related to cyclic AMP and a tumor promoter, phorbol ester, on intercellular communications via gap junctions were investigated by the Lucifer Yellow-transfer method in cultured rabbit gastric epithelial cells. Cells were in contact with each drug for 4 hr before the microinjection of the dye into a cell. Dye transfer capacity was significantly increased by dibutyryl cyclic AMP (10(-3) M), theophylline (10(-3) M), 3-isobutyl-1-methylxanthine (10(-4) M), forskolin (10(-6) M) and irsogladine (10(-4) M); and it was inhibited by 12-O-tetradecanoyl-phorbol-13-acetate (100 ng/ml). These results suggest that the intercellular communication between cultured rabbit gastric epithelial cells is upregulated by cyclic AMP.

Topics: Animals; Anti-Ulcer Agents; Cell Communication; Cells, Cultured; Cyclic AMP; Epithelial Cells; Epithelium; Extracellular Space; Gastric Mucosa; Intercellular Junctions; Isoquinolines; Microscopy, Electron; Rabbits; Stomach; Triazines; Up-Regulation