lucifer-yellow has been researched along with acrylodan* in 1 studies
1 other study(ies) available for lucifer-yellow and acrylodan
| Article | Year |
|---|---|
Sulfhydryl-selective fluorescence labeling of lipoprotein(a) reveals evidence for one single disulfide linkage between apoproteins(a) and B-100.
Human lipoprotein(a) and low-density lipoprotein were labeled with two different sulfhydryl-selective fluorescence markers. The hydrophilic fluorophore lucifer yellow iodoacetamide and the apolar compound 6-acryloyl-2-(dimethylamino)naphthalene were used to derivatize free -SH groups in the lipoproteins. Three sulfhydryls could be detected in low-density lipoprotein, whereas only two cysteines were available in lipoprotein(a). One of the three -SH groups in low-density lipoprotein was shown to be located in close proximity to the particle surface. We suggest that this surface-exposed cysteine of apoprotein B-100 serves as a component for the disulfide linkage to apoprotein(a) in lipoprotein(a). Topics: 2-Naphthylamine; Apolipoprotein B-100; Apolipoproteins B; Binding Sites; Disulfides; Humans; Isoquinolines; Lipoprotein(a); Lipoproteins; Protein Binding; Protein Conformation; Spectrometry, Fluorescence | 1991 |