losartan-potassium has been researched along with benzidine* in 1 studies
1 other study(ies) available for losartan-potassium and benzidine
| Article | Year |
|---|---|
Comparison of gene expression profiles in erythroid-like cells derived from mouse embryonic stem cells differentiated in simple and co-culture systems.
The feeder layer and the presence of specific growth factors are thought to induce the differentiation of embryonic stem cells (ESCs) in culture. The aim of this study was to evaluate the effect of erythropoietin (EPO) on the differentiation of ESCs into erythroid colonies in simple and co-culture systems. Embryoid bodies were dissociated and replated in semisolid medium in simple culture or in a co-culture system with bone-marrow stromal cells (BMSCs), both in the presence or absence of EPO. Colony assays, benzidine staining, and ultrastructural studies were carried out until day 10 of culture. Expression of the epsilon globin, betaH1 globin, runt-related transcription factor 1 (RUNX1), betamajor globin, and erythropoietin receptor (EPOR) genes was evaluated using semi-quantitative RT-PCR. A comparison with the corresponding controls showed that colony size increased in both systems (P Topics: Animals; Benzidines; Bone Marrow Cells; Cell Culture Techniques; Cell Differentiation; Coculture Techniques; DNA Primers; Embryonic Stem Cells; Erythropoietin; Fibronectins; Gene Expression Profiling; Mice; Mitosis; Octamer Transcription Factor-3; Reverse Transcriptase Polymerase Chain Reaction | 2008 |