losartan-potassium has been researched along with 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate* in 2 studies
2 other study(ies) available for losartan-potassium and 3-((3-cholamidopropyl)dimethylammonium)-1-propanesulfonate
Article | Year |
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Characterisation of CHAPS-solubilised active erythropoietin receptors from Rauscher erythroleukaemic cells using biotinylated erythropoietin.
Topics: Animals; Autoradiography; Biotin; Cholic Acids; Detergents; Electrophoresis, Polyacrylamide Gel; Erythropoietin; Iodine Radioisotopes; Leukemia, Experimental; Mice; Rauscher Virus; Receptors, Cell Surface; Receptors, Erythropoietin; Solubility | 1991 |
Solubilization and characterization of erythropoietin receptor from transplantable mouse erythroblastic leukemic cells.
Functional erythropoietin (Ep) receptor (Ep-R) on transplantable mouse leukemic cells was solubilized by 3[(3-cholamidopropyl)dimethyl ammonio]-1-propanesulfate (CHAPS). An assay of the solubilized Ep-R was established: (1) radioiodinated Ep was bound to the solubilized Ep-R, (2) Ep.Ep-R complexes were covalently cross-linked, (3) the cross-linked products were selectively precipitated by polyethylene glycol, (4) the products were separated on sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and (5) the Ep.Ep-R cross-linked products were measured. Optimal conditions to yield the cross-linked products and to stabilize the solubilized Ep-R were found. The solubilized Ep-R had properties similar to those of Ep-R on the membrane with respect to binding with Ep and the cross-linked products. These results would be exploited to allow purification of Ep-R. Topics: Animals; Cell Membrane; Chemical Precipitation; Cholic Acids; Cross-Linking Reagents; Electrophoresis, Polyacrylamide Gel; Erythropoietin; Leukemia, Erythroblastic, Acute; Mice; Mice, Inbred C3H; Neoplasm Transplantation; Polyethylene Glycols; Receptors, Cell Surface; Receptors, Erythropoietin; Solubility; Succinimides | 1989 |