lithium-chloride and oxophenylarsine

Extracellularly applied sodium orthovanadate (30-3000 microM) evoked oscillatory inward Cl- currents in defolliculated Xenopus laevis oocytes. The current responses were attenuated by microinjection of EGTA into the oocytes and by treatment of the oocytes with pertussis toxin (2 micrograms/ml). The vanadate responses were not affected by preceding vanadate (1 mM) responses or an angiotensin II (200 nM) response, or by pre-application of atropine (5 microM). Intracellular injection of vanadate was ineffective. These results suggest that vanadate stimulates Ca2+ mobilization in Xenopus oocytes possibly by activating surface membrane receptors, which is coupled to pertussis toxin-sensitive G-protein.

Topics: Angiotensin II; Animals; Arsenicals; Atropine; Calcium; Chloride Channels; Collagenases; Electrophysiology; Female; GTP-Binding Proteins; In Vitro Techniques; Lithium Chloride; Oocytes; Patch-Clamp Techniques; Pertussis Toxin; Vanadates; Virulence Factors, Bordetella; Xenopus laevis