lithium-chloride and 3-aminocyclopentane-1-carboxylic-acid

This study examined the ability of the excitatory amino acid glutamate and its analogs to stimulate phosphatidylinositol metabolism in isolated cochlear nucleus tissue from young chicks. In the presence of lithium chloride, glutamate and (+/-)-1-aminocyclopentyl-trans-1,3-dicarboxylate (ACPD) stimulated the formation of inositol phosphates to levels significantly above unstimulated control levels. Unexpectedly, quisqualate did not stimulate inositol phosphates formation. The N-methyl-D-aspartate (NMDA) receptor antagonist 2-amino-5-phosphonovalerate (APV), the ionotropic kainate/quisqualate receptor antagonist 6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the putative metabotropic glutamate receptor antagonist 2-amino-3-phosphonopropionate (AP3) had no effect on the glutamate stimulated formation of inositol phosphates. We conclude that a metabotropic glutamate receptor is present on cochlear nucleus neurons of posthatch chicks and is able to stimulate formation of inositol phosphates.

Topics: 2-Amino-5-phosphonovalerate; 6-Cyano-7-nitroquinoxaline-2,3-dione; Animals; Chickens; Cochlear Nucleus; Cyclopentanes; Excitatory Amino Acid Antagonists; Glutamates; Glutamic Acid; In Vitro Techniques; Inositol Phosphates; Lithium Chloride; Phosphatidylinositols; Quinoxalines; Quisqualic Acid; Receptors, Glutamate; Receptors, N-Methyl-D-Aspartate