liriodendrin and syringin

To study the chemical constituents of Syringa veutina Kom.. The constituents were isolated and repeatedly purified on silica and Sephadex LH-20 column chromatography. They were dentified and structurally elucidated by spectral analysis.. Seven compounds were obtained. They were identified as Syningin(1), Liriodendrin(2), (+) -Medioresinol di-O-beta-D-glucopyranosied (3), Rutin(4), Quercetin 3-O-beta-D-glucopyranoside (5), Kaempferol-3-O-beta-D-glucopyranosied (6), D-mannitol (7).. Compound 2 and 3 were isolated from this genus for the first time. Compound 4, 5, 6 and 7 were isolated from this plant for the first time.

Topics: Furans; Glucosides; Magnetic Resonance Spectroscopy; Phenylpropionates; Plant Leaves; Plants, Medicinal; Quercetin; Rutin; Syringa

A high performance liquid chromatography (HPLC) method was developed for the first time to quantify simultaneously the six major active ingredients in Acanthopanax senticosus (Rupr. et Maxim.) Harms, namely protocatechuic acid, syringin, chlorogenic acid, caffeic acid, liriodendrin and isofraxidin. The analysis was performed by a reverse phase gradient elution with an aqueous mobile phase (containing 0.05% phosphoric acid) modified by acetonitrile and diode-array multiple-wavelength UV detector (DAD). Six regression equations showed good linear relationships between the peak area of each marker and concentration. The recoveries of the markers listed above were 92.3%, 93.9%, 90.3%, 93.1%, 94.3% and 90.7%, respectively. The relative standard deviation of intra-day and inter-day were less than 2.7% and 3.1%, respectively. This method was validated for specificity, accuracy, precision and limits of quantification. Medicinal materials of ten commercial brands were analyzed and found to contain different amounts of the six bioactive markers. The method developed can be used for the quality control of Acanthopanax senticosus (Rupr. et Maxim.) Harms.

Topics: Caffeic Acids; Calibration; Chlorogenic Acid; Chromatography, High Pressure Liquid; Coumarins; Eleutherococcus; Furans; Glucosides; Hydroxybenzoates; Indicators and Reagents; Phenylpropionates; Quality Control; Reference Standards; Solutions; Spectrophotometry, Ultraviolet

When liriodendrin or syringin was incubated for 24 h with human intestinal bacteria, two metabolites, (+)-syringaresinol-beta-D-glucopyranoside and (+)-syringaresinol, from liriodendrin and one metabolite, synapyl alcohol, from syringin were produced. The metabolic time course of liriodendrin was as follows: at early time, liriodendrin was converted to (+)-syringaresinol-beta-D-glucopyranoside, and then (+)-syringaresinol. The in vitro cytotoxicities of these metabolites, (+)-syringaresinol and synapyl alcohol, were superior to those of liriodendrin and syringin.

Topics: Antineoplastic Agents, Phytogenic; Bacteria; Cell Survival; Furans; Glucosides; Humans; Intestines; Magnetic Resonance Spectroscopy; Mass Spectrometry; Phenylpropionates; Tumor Cells, Cultured