lipoxin-a5 and 15-hydroperoxy-5-8-11-14-17-eicosapentaenoic-acid

lipoxin-a5 has been researched along with 15-hydroperoxy-5-8-11-14-17-eicosapentaenoic-acid* in 2 studies

Other Studies

2 other study(ies) available for lipoxin-a5 and 15-hydroperoxy-5-8-11-14-17-eicosapentaenoic-acid

Article	Year
Transformation of 15-hydroperoxyeicosapentaenoic acid to lipoxin A5 and B5, mono- and dihydroxyeicosapentaenoic acids by porcine leukocytes. Biochimica et biophysica acta, 1987, Feb-23, Volume: 917, Issue:3 15-Hydroperoxy[1-14C]eicosapentaenoic acid derived from eicosapentaenoic acid (EPA) was incubated with suspensions of porcine leukocytes. Incubation with porcine leukocytes resulted in the formation of seven dihydroxy compounds, one monohydroxy and one hydroxyepoxy compound. After separation by reverse-phase and straight-phase HPLC, GC/MS analysis revealed that these metabolites were four isomers of 8,15-diHEPEs, two isomers of 14,15-diHEPEs, one isomer of 5,15-diHEPE, 15-HEPE and an epoxyalcohol: 13-hydroxy-14,15-epoxyeicosatetraenoic acid. In addition to the above metabolites, two trihydroxytetraene derivatives were also isolated. GC/MS and ultraviolet spectroscopy identified the two trihydroxypentaene derivatives as 5,6,15-trihydroxy-7,9,11,13,17-eicosapentaenoic acid (lipoxin A5) and 5,14,15-trihydroxy-6,8,10,12,17-eicosapentaenoic acid (lipoxin B5). This study demonstrated that the 15-hydroperoxide of EPA can be actively converted to various hydroxylated products via the 5-, 12- and 15-lipoxygenase as well as epoxyisomerase pathways in the porcine leukocytes. Topics: Animals; Biotransformation; Chromatography, High Pressure Liquid; Eicosapentaenoic Acid; Gas Chromatography-Mass Spectrometry; In Vitro Techniques; Leukocytes; Lipoxins; Spectrophotometry, Ultraviolet; Swine	1987
Lipoxene: a new group of trihydroxy pentaenes of eicosapentaenoic acid derived from porcine leukocytes. Biochemical and biophysical research communications, 1985, Jan-31, Volume: 126, Issue:2 Incubation of porcine leukocytes with [1-14C]-15-hydroperoxyeicosapentaenoic acid (15-HPEPE) results in the formation of a group of polar metabolites which after separation and purification by RP-HPLC and SP-HPLC were found to be a series of new compounds containing three hydroxy groups and four conjugated double bonds. The structures of these new metabolites were established by U. V. spectrophotometry and GC/MS to be trihydroxy pentaenes of EPA, i.e., 5,6,15-trihydroxy-7,9,11,13,17-eicosapentaenoic acid and 5,14,15-trihydroxy-6,8,10,12,17-eicosapentaenoic acid. Because of the additional double bond at C17-18, these two new metabolites of EPA were proposed to be lipoxene A and lipoxene B, respectively. Topics: Animals; Chromatography, High Pressure Liquid; Eicosapentaenoic Acid; Fatty Acids, Unsaturated; Gas Chromatography-Mass Spectrometry; In Vitro Techniques; Leukocytes; Lipoxins; Spectrophotometry, Ultraviolet; Swine	1985

Article

Year

Transformation of 15-hydroperoxyeicosapentaenoic acid to lipoxin A5 and B5, mono- and dihydroxyeicosapentaenoic acids by porcine leukocytes.

Biochimica et biophysica acta, 1987, Feb-23, Volume: 917, Issue:3

15-Hydroperoxy[1-14C]eicosapentaenoic acid derived from eicosapentaenoic acid (EPA) was incubated with suspensions of porcine leukocytes. Incubation with porcine leukocytes resulted in the formation of seven dihydroxy compounds, one monohydroxy and one hydroxyepoxy compound. After separation by reverse-phase and straight-phase HPLC, GC/MS analysis revealed that these metabolites were four isomers of 8,15-diHEPEs, two isomers of 14,15-diHEPEs, one isomer of 5,15-diHEPE, 15-HEPE and an epoxyalcohol: 13-hydroxy-14,15-epoxyeicosatetraenoic acid. In addition to the above metabolites, two trihydroxytetraene derivatives were also isolated. GC/MS and ultraviolet spectroscopy identified the two trihydroxypentaene derivatives as 5,6,15-trihydroxy-7,9,11,13,17-eicosapentaenoic acid (lipoxin A5) and 5,14,15-trihydroxy-6,8,10,12,17-eicosapentaenoic acid (lipoxin B5). This study demonstrated that the 15-hydroperoxide of EPA can be actively converted to various hydroxylated products via the 5-, 12- and 15-lipoxygenase as well as epoxyisomerase pathways in the porcine leukocytes.

Topics: Animals; Biotransformation; Chromatography, High Pressure Liquid; Eicosapentaenoic Acid; Gas Chromatography-Mass Spectrometry; In Vitro Techniques; Leukocytes; Lipoxins; Spectrophotometry, Ultraviolet; Swine

1987

Lipoxene: a new group of trihydroxy pentaenes of eicosapentaenoic acid derived from porcine leukocytes.

Biochemical and biophysical research communications, 1985, Jan-31, Volume: 126, Issue:2

Incubation of porcine leukocytes with [1-14C]-15-hydroperoxyeicosapentaenoic acid (15-HPEPE) results in the formation of a group of polar metabolites which after separation and purification by RP-HPLC and SP-HPLC were found to be a series of new compounds containing three hydroxy groups and four conjugated double bonds. The structures of these new metabolites were established by U. V. spectrophotometry and GC/MS to be trihydroxy pentaenes of EPA, i.e., 5,6,15-trihydroxy-7,9,11,13,17-eicosapentaenoic acid and 5,14,15-trihydroxy-6,8,10,12,17-eicosapentaenoic acid. Because of the additional double bond at C17-18, these two new metabolites of EPA were proposed to be lipoxene A and lipoxene B, respectively.

Topics: Animals; Chromatography, High Pressure Liquid; Eicosapentaenoic Acid; Fatty Acids, Unsaturated; Gas Chromatography-Mass Spectrometry; In Vitro Techniques; Leukocytes; Lipoxins; Spectrophotometry, Ultraviolet; Swine

1985