lipoteichoic-acid and neamine

Amphiphilic aminoglycoside derivatives are potential new antimicrobial agents mostly developed to fight resistant bacteria. The mechanism of action of the 3',6-dinonyl neamine, one of the most promising derivative, has been investigated on Gram-negative bacteria, including P. aeruginosa. In this study, we have assessed its mechanism of action against Gram-positive bacteria, S. aureus and B. subtilis. By conducting time killing experiments, we assessed the bactericidal effect induced by 3',6-dinonyl neamine on S. aureus MSSA and MRSA. By measuring the displacement of BODIPY™-TR cadaverine bound to lipoteichoic acids (LTA), we showed that 3',6-dinonyl neamine interacts with these bacterial surface components. We also highlighted the ability of 3',6-dinonyl neamine to enhance membrane depolarization and induce membrane permeability, by using fluorescent probes, DiSC

Topics: Anti-Bacterial Agents; Bacillus subtilis; Cell Membrane; Cell Membrane Permeability; Framycetin; Gram-Positive Bacteria; Lipopolysaccharides; Microbial Sensitivity Tests; Staphylococcus aureus; Structure-Activity Relationship; Surface-Active Agents; Teichoic Acids