lipofectamine and 6-carboxyfluorescein

lipofectamine has been researched along with 6-carboxyfluorescein* in 1 studies

Other Studies

1 other study(ies) available for lipofectamine and 6-carboxyfluorescein

Article	Year
Selection of DNA-Encoded Libraries to Protein Targets within and on Living Cells. Journal of the American Chemical Society, 2019, 10-30, Volume: 141, Issue:43 We report the selection of DNA-encoded small molecule libraries against protein targets within the cytosol and on the surface of live cells. The approach relies on generation of a covalent linkage of the DNA to protein targets by affinity labeling. This cross-linking event enables subsequent copurification by a tag on the recombinant protein. To access targets within cells, a cyclic cell-penetrating peptide is appended to DNA-encoded libraries for delivery across the cell membrane. As this approach assesses binding of DELs to targets in live cells, it provides a strategy for selection of DELs against challenging targets that cannot be expressed and purified as active. Topics: Cell-Penetrating Peptides; Cross-Linking Reagents; Cytosol; DNA; Fluoresceins; HEK293 Cells; Humans; Lipids; Peptides, Cyclic; Polymerase Chain Reaction; Proteins; Recombinant Fusion Proteins; Small Molecule Libraries; Tetrahydrofolate Dehydrogenase; Transfection; Trimethoprim	2019

Article

Year

Selection of DNA-Encoded Libraries to Protein Targets within and on Living Cells.

Journal of the American Chemical Society, 2019, 10-30, Volume: 141, Issue:43

We report the selection of DNA-encoded small molecule libraries against protein targets within the cytosol and on the surface of live cells. The approach relies on generation of a covalent linkage of the DNA to protein targets by affinity labeling. This cross-linking event enables subsequent copurification by a tag on the recombinant protein. To access targets within cells, a cyclic cell-penetrating peptide is appended to DNA-encoded libraries for delivery across the cell membrane. As this approach assesses binding of DELs to targets in live cells, it provides a strategy for selection of DELs against challenging targets that cannot be expressed and purified as active.

Topics: Cell-Penetrating Peptides; Cross-Linking Reagents; Cytosol; DNA; Fluoresceins; HEK293 Cells; Humans; Lipids; Peptides, Cyclic; Polymerase Chain Reaction; Proteins; Recombinant Fusion Proteins; Small Molecule Libraries; Tetrahydrofolate Dehydrogenase; Transfection; Trimethoprim

2019