lipid-a and dansyl-hydrazine

lipid-a has been researched along with dansyl-hydrazine* in 2 studies

Other Studies

2 other study(ies) available for lipid-a and dansyl-hydrazine

Article	Year
Two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. I. LPS activation with cyanogen bromide. Methods in molecular biology (Clifton, N.J.), 2011, Volume: 739 This chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a smooth(S)-form lipopolysaccharide (LPS). LPS is modified by the activation of the hydroxyl groups present in its O-antigen moiety with cyanogen bromide in aqueous acetone. The method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid A moiety. Conjugation of smooth-form LPS from Salmonella enterica sv. Minnesota with dansyl hydrazine and horseradish -peroxidase yields labeling ratios above 300 nmol dansyl per mg LPS, with nearly no loss of the original endotoxin activity. In the case of horseradish peroxidase, introducing a spacer, a ratio of 28 nmol HRP per mg LPS is obtained, preserving 65% of the original endotoxic activity. Topics: Amines; Chemistry Techniques, Analytical; Cyanogen Bromide; Dansyl Compounds; Electrophoresis, Polyacrylamide Gel; Horseradish Peroxidase; Hydrazines; Lipid A; O Antigens; Salmonella enterica; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	2011
Two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. II. LPS activation with a cyanopyridinium agent. Methods in molecular biology (Clifton, N.J.), 2011, Volume: 739 This chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a lipopolysaccharide (LPS). LPS is modified by the activation of the hydroxyl groups present in its O-antigen moiety with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP). The method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid A moiety. Conjugation of smooth-form LPS from Salmonella enterica sv. Minnesota with dansyl hydrazine and horseradish peroxidase yields labeling ratios above 110 nmol dansyl/mg LPS, with nearly no loss of the original endotoxic activity. In the case of horseradish peroxidase, introducing a spacer, a ratio of 29 nmol HRP/mg LPS was obtained, preserving 65% of the original endotoxic activity and an enzymatic activity of 120 U/mg. Topics: Amines; Dansyl Compounds; Fluorescent Dyes; Horseradish Peroxidase; Hydrazines; Lipid A; Nitriles; O Antigens; Pyridinium Compounds; Salmonella enterica; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization	2011

Article

Year

Two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. I. LPS activation with cyanogen bromide.

Methods in molecular biology (Clifton, N.J.), 2011, Volume: 739

This chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a smooth(S)-form lipopolysaccharide (LPS). LPS is modified by the activation of the hydroxyl groups present in its O-antigen moiety with cyanogen bromide in aqueous acetone. The method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid A moiety. Conjugation of smooth-form LPS from Salmonella enterica sv. Minnesota with dansyl hydrazine and horseradish -peroxidase yields labeling ratios above 300 nmol dansyl per mg LPS, with nearly no loss of the original endotoxin activity. In the case of horseradish peroxidase, introducing a spacer, a ratio of 28 nmol HRP per mg LPS is obtained, preserving 65% of the original endotoxic activity.

Topics: Amines; Chemistry Techniques, Analytical; Cyanogen Bromide; Dansyl Compounds; Electrophoresis, Polyacrylamide Gel; Horseradish Peroxidase; Hydrazines; Lipid A; O Antigens; Salmonella enterica; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2011

Two efficient methods for the conjugation of smooth-form lipopolysaccharides with probes bearing hydrazine or amino groups. II. LPS activation with a cyanopyridinium agent.

Methods in molecular biology (Clifton, N.J.), 2011, Volume: 739

This chapter presents a conjugation method for coupling probes bearing hydrazine or primary amino groups to a lipopolysaccharide (LPS). LPS is modified by the activation of the hydroxyl groups present in its O-antigen moiety with 1-cyano-4-dimethylaminopyridinium tetrafluoroborate (CDAP). The method yields conjugates with good labeling ratios, preserving the endotoxic activity of the lipid A moiety. Conjugation of smooth-form LPS from Salmonella enterica sv. Minnesota with dansyl hydrazine and horseradish peroxidase yields labeling ratios above 110 nmol dansyl/mg LPS, with nearly no loss of the original endotoxic activity. In the case of horseradish peroxidase, introducing a spacer, a ratio of 29 nmol HRP/mg LPS was obtained, preserving 65% of the original endotoxic activity and an enzymatic activity of 120 U/mg.

Topics: Amines; Dansyl Compounds; Fluorescent Dyes; Horseradish Peroxidase; Hydrazines; Lipid A; Nitriles; O Antigens; Pyridinium Compounds; Salmonella enterica; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2011