lipid-a has been researched along with 9-anthroic-acid* in 1 studies
1 other study(ies) available for lipid-a and 9-anthroic-acid
| Article | Year |
|---|---|
Development of a new quantitative method for detection of endotoxin by fluorescence labeling of 3-hydroxy fatty acid.
New quantitative method for the detection of minute amounts of endotoxin has been developed using 3-hydroxytetradecanoic acid as a chemical marker. After converting 3-hydroxytetradecanoic acid to methyl ester, it was coupled with a fluorescent probe, anthracene-9-carboxyl chloride, obtained by chlorization of 9-anthroic acid with oxalyl chloride. The resulting ester was isolated by HPLC on silica column. The purified product, methyl-3-0-(9-carboxy-anthracenyl) tetradecanoate (M/Z 462), was highly responsive to a fluorescence spectrophotometer, showing maximum emission with excitation wavelength at 257 nm and emission wavelength at 458 nm in dichloromethane, the limit of detection being as little as 10 f mol. Using this method it is currently possible to detect Salmonella abortus equi endotoxin in aqueous solution at a level of 100 pg. Topics: Anthracenes; Chromatography, Gas; Chromatography, High Pressure Liquid; Endotoxins; Fatty Acids; Fluorescence; Lipid A; Mass Spectrometry; Myristic Acids; Polysaccharides, Bacterial; Salmonella | 1990 |