linoleic-acid-hydroperoxide has been researched along with 3-(dimethylamino)benzoic-acid* in 1 studies
1 other study(ies) available for linoleic-acid-hydroperoxide and 3-(dimethylamino)benzoic-acid
Article | Year |
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Colorimetric method for the determination of lipoxygenase activity.
A colorimetic assay for lipoxygenase activity has been developed. The assay is based on the detection of the lipoxygenase reaction product, linoleic acid hydroperoxide, by the oxidative coupling of 3-methyl-2-benzothiazolinone (MBTH) with 3-(dimethylamino)benzoic acid (DMAB) in a hemoglobin-catalyzed reaction. This test reaction is rapid and sensitive, and it offers advantages over other methods for detecting lipoxygenase activity. The assay is capable of detecting activity in a number of crude vegetable homogenates and should be particularly useful where a rapid visual determination of lipoxygenase activity is desired. Topics: Aminobenzoates; Benzothiazoles; Colorimetry; Hemoglobins; Hydrazones; Indicators and Reagents; Linoleic Acids; Lipid Peroxides; Lipoxygenase; meta-Aminobenzoates; Oxidation-Reduction; Plant Extracts; Solutions; Thiazoles; Vegetables | 2001 |