linoleic-acid and totarol

Diterpenoids, totarol (1), totaradiol (2), 19-hydroxytotarol (3), totaral (4), 4 beta-carboxy-19-nortotarol (5), sugiol (6), isolated from Podocarpus nagi, were evaluated as antioxidants. Microsomal lipid peroxidation induced by Fe(III)-ADP/NADPH and mitochondrial lipid peroxidation induced by Fe(III)-ADP/ NADH were inhibited by these terpenoids. They inhibited linoleic acid autoxidation but not generation of superoxide anion. Totarol (1) protected mitochondrial respiratory enzyme activities against NADPH induced oxidative injury. Totarane diterpenes from P. nagi were shown to be effective to protect biological systems and function against various oxidative stresses.

Topics: Abietanes; Animals; Antioxidants; Diterpenes; Japan; Linoleic Acid; Linoleic Acids; Lipid Peroxidation; Microsomes, Liver; NADH Dehydrogenase; NADP; Oxidation-Reduction; Plant Roots; Plants, Medicinal; Rats; Succinate Cytochrome c Oxidoreductase; Superoxides

A diterpenoid, totarol (1), from Podocarpus nagi was evaluated as an antioxidant. This diterpenoid inhibited autoxidation of linoleic acid. Mitochondrial and microsomal lipid peroxidation induced by Fe(III)-ADP/NADH or Fe(III)-ADP/NADPH were also inhibited. Nagilactone E (2), a norditerpene lactone isolated from the same source, had no antioxidative activity. Furthermore, totarol protected red cells against oxidative hemolysis. This diterpene was shown to be effective in protecting biological systems against oxidative stresses.

Topics: Abietanes; Adenosine Diphosphate; Animals; Antioxidants; Diterpenes; Erythrocyte Membrane; Humans; Linoleic Acid; Linoleic Acids; Lipid Peroxidation; Male; Membrane Lipids; Microsomes, Liver; Mitochondria, Liver; NAD; NADP; Oxidation-Reduction; Rats; Rats, Wistar