linoleic-acid and tetracosapentaenoic-acid

linoleic-acid has been researched along with tetracosapentaenoic-acid* in 2 studies

Other Studies

2 other study(ies) available for linoleic-acid and tetracosapentaenoic-acid

Article	Year
Evidence of isozymes for delta6 fatty acid desaturase in rat hepatocytes. Bioscience, biotechnology, and biochemistry, 2003, Volume: 67, Issue:2 The expression of delta6 fatty acid desaturase, previously identified, was suppressed almost completely by hyper expression of the corresponding antisense gene in a transformant of the rat hepatic cell line BRL-3A. Conversion rates of [1-14C] linoleic acid, alpha-linolenic acid, and tetracosapentaenoic acid into the respective delta6 fatty acids were equivalent to those in control cells. This finding suggested that all of these reactions were catalyzed by at least two delta6 desaturase isozymes in rat hepatocytes. Topics: alpha-Linolenic Acid; Animals; Antisense Elements (Genetics); Blotting, Western; Carbon Radioisotopes; Cell Line; Docosahexaenoic Acids; Fatty Acid Desaturases; Fatty Acids, Unsaturated; Gene Expression; Hepatocytes; Isoenzymes; Linoleic Acid; Rats; Transformation, Genetic	2003
Effect of the delta6-desaturase inhibitor SC-26196 on PUFA metabolism in human cells. Lipids, 2003, Volume: 38, Issue:4 The objective of this study was to determine the effect of 2,2-diphenyl-5-(4-[[(1 E)-pyridin-3-yl-methylidene]amino]piperazin-1-yl)pentanenitrile (SC-26196), a delta6-desaturase inhibitor, on PUFA metabolism in human cells. SC-26196 inhibited the desaturation of 2 microM [1-14C] 18:2n-6 by 87-95% in cultured human skin fibroblasts, coronary artery smooth muscle cells, and astrocytes. By contrast, SC-26196 did not affect the conversion of [1-14C]20:3n-6 to 20:4 in the fibroblasts, demonstrating that it is selective for delta6-desaturase. The IC50 values for inhibition of the desaturation of 2 microM [1-14C] 18:3n-3 and [3-14C]24:5n-3 in the fibroblasts, 0.2-0.4 microM, were similar to those for the inhibition of [1-14C 18:2n-6 desaturation, and the rates of recovery of [1-14C]18:2n-6 and [3-14C]24:5n-3 desaturation after removal of SC-26196 from the culture medium also were similar. SC-26196 reduced the conversion of [3-14C]22:5n-3 and [3-14C]24:5n-3 to DHA by 75 and 84%, respectively, but it had no effect on the retroconversion of [3-14C]24:6n-3 to DHA. These results demonstrate that SC-26196 effectively inhibits the desaturation of 18- and 24-carbon PUFA and, therefore, decreases the synthesis of arachidonic acid, EPA, and DHA in human cells. Furthermore, they provide additional evidence that the conversion of 22:5n-3 to DHA involves delta6-desaturation. Topics: Carbon Radioisotopes; Cell Line; Docosahexaenoic Acids; Enzyme Inhibitors; Fatty Acid Desaturases; Fatty Acids, Unsaturated; Humans; Linoleic Acid; Piperazines; Stearoyl-CoA Desaturase; Time Factors	2003

Article

Year

Evidence of isozymes for delta6 fatty acid desaturase in rat hepatocytes.

Bioscience, biotechnology, and biochemistry, 2003, Volume: 67, Issue:2

The expression of delta6 fatty acid desaturase, previously identified, was suppressed almost completely by hyper expression of the corresponding antisense gene in a transformant of the rat hepatic cell line BRL-3A. Conversion rates of [1-14C] linoleic acid, alpha-linolenic acid, and tetracosapentaenoic acid into the respective delta6 fatty acids were equivalent to those in control cells. This finding suggested that all of these reactions were catalyzed by at least two delta6 desaturase isozymes in rat hepatocytes.

Topics: alpha-Linolenic Acid; Animals; Antisense Elements (Genetics); Blotting, Western; Carbon Radioisotopes; Cell Line; Docosahexaenoic Acids; Fatty Acid Desaturases; Fatty Acids, Unsaturated; Gene Expression; Hepatocytes; Isoenzymes; Linoleic Acid; Rats; Transformation, Genetic

2003

Effect of the delta6-desaturase inhibitor SC-26196 on PUFA metabolism in human cells.

Lipids, 2003, Volume: 38, Issue:4

The objective of this study was to determine the effect of 2,2-diphenyl-5-(4-[[(1 E)-pyridin-3-yl-methylidene]amino]piperazin-1-yl)pentanenitrile (SC-26196), a delta6-desaturase inhibitor, on PUFA metabolism in human cells. SC-26196 inhibited the desaturation of 2 microM [1-14C] 18:2n-6 by 87-95% in cultured human skin fibroblasts, coronary artery smooth muscle cells, and astrocytes. By contrast, SC-26196 did not affect the conversion of [1-14C]20:3n-6 to 20:4 in the fibroblasts, demonstrating that it is selective for delta6-desaturase. The IC50 values for inhibition of the desaturation of 2 microM [1-14C] 18:3n-3 and [3-14C]24:5n-3 in the fibroblasts, 0.2-0.4 microM, were similar to those for the inhibition of [1-14C 18:2n-6 desaturation, and the rates of recovery of [1-14C]18:2n-6 and [3-14C]24:5n-3 desaturation after removal of SC-26196 from the culture medium also were similar. SC-26196 reduced the conversion of [3-14C]22:5n-3 and [3-14C]24:5n-3 to DHA by 75 and 84%, respectively, but it had no effect on the retroconversion of [3-14C]24:6n-3 to DHA. These results demonstrate that SC-26196 effectively inhibits the desaturation of 18- and 24-carbon PUFA and, therefore, decreases the synthesis of arachidonic acid, EPA, and DHA in human cells. Furthermore, they provide additional evidence that the conversion of 22:5n-3 to DHA involves delta6-desaturation.

Topics: Carbon Radioisotopes; Cell Line; Docosahexaenoic Acids; Enzyme Inhibitors; Fatty Acid Desaturases; Fatty Acids, Unsaturated; Humans; Linoleic Acid; Piperazines; Stearoyl-CoA Desaturase; Time Factors

2003