linoleic-acid has been researched along with lycorine* in 1 studies
1 other study(ies) available for linoleic-acid and lycorine
| Article | Year |
|---|---|
Rapid identification of inhibitors that interfere with poliovirus replication using a cell-based assay.
A small molecule library containing 480 known bioactive compounds was screened for antiviral activity against poliovirus (PV) using a cellular fluorescence resonance energy transfer (FRET) assay for viral protease activity. The infected reporter cells treated with the viral replication-suppressing compounds were examined via fluorescence microscope 7.5 h postinfection. Twelve molecules showed moderate to potent antiviral activity at concentrations less than 32 microM during the primary screening. Three compounds, anisomycin, linoleic acid, and lycorine, were chosen for validation. A dose-dependent cytotoxicity assay and a secondary screening using conventional plaque assay were conducted to confirm the results. The developed method can be used for rapid screening for molecules with antiviral activity. Topics: Amaryllidaceae Alkaloids; Animals; Anisomycin; Antiviral Agents; Cell Line; Chlorocebus aethiops; Fluorescence Resonance Energy Transfer; Linoleic Acid; Microbial Sensitivity Tests; Microscopy, Fluorescence; Peptide Hydrolases; Phenanthridines; Poliovirus; Viral Plaque Assay; Viral Proteins; Virus Replication | 2008 |