linoleic-acid and linoleyl-alcohol

To extract and analyze the liposoluble chemical constituents of the petroleum ether extraction and alcohol extraction from fruits of Harrisonia perforata.. To analyze the liposoluble components by GC-MS.. 29 compounds were identified from petroleum ether extraction, amounting to 69.67% of the total detected constituents and the ir relative contents were confirmed. From the alcohol extraction, 12 compounds were identified, amounting to 71.00% of the total detected constituents and the ir relative contents were also confirmed.. Linoleic acid, Palmitic acid, beta-Stitosterol are common liposoluble components in two extractions.

Topics: Fatty Alcohols; Fruit; Gas Chromatography-Mass Spectrometry; Hot Temperature; Linoleic Acid; Oleic Acids; Palmitic Acid; Plants, Medicinal; Simaroubaceae; Sitosterols

We have studied the aerobic oxidation of linoleyl alcohol (LAL) by potato tuber lipoxygenase in the presence of 0.02% (w/v) non-ionic detergent Lubrol PX (and its analog C12E10) and 0.1 mM sodium dodecyl sulfate to investigate the role of carboxylic group in substrate binding. While the enzyme displayed a comparable affinity toward LA and LAL, the rate of LAL oxidation was approximately one-fourth of that of linoleic acid. The pH-profile of the reaction suggests that the rate of LAL oxidation is controlled by two ionizable groups with pKa values of 5.3 and 7.5, with optimal pH being 6.4+/-0.1. Since LAL is not ionizable at this pH, we conclude that the rate of the reaction is controlled by two ionogenic groups of the enzyme. The primary dioxygenation product(s) of LAL had a maximal absorbance at 233+/-1 nm. The products have been isolated, catalytically hydrogenated with H2 over Pd on carbon, and analyzed by GC-MS. Two major equimolar products were found to be 9- and 13-hydroxystearyl alcohols, indicating that 9- and 13-hydroperoxylinoleyl alcohols are the primary dioxygenation products. Based on these results we propose that the carboxyl group of polyunsaturated fatty acid may not be involved in substrate binding of potato tuber lipoxygenase.

Topics: Binding Sites; Detergents; Fatty Alcohols; Gas Chromatography-Mass Spectrometry; Hydrogen-Ion Concentration; Linoleic Acid; Lipoxygenase; Oxidation-Reduction; Polidocanol; Polyethylene Glycols; Sodium Dodecyl Sulfate; Solanum tuberosum; Solubility; Substrate Specificity