linoleic-acid and cobamamide

linoleic-acid has been researched along with cobamamide* in 1 studies

Other Studies

1 other study(ies) available for linoleic-acid and cobamamide

ArticleYear
Alpha-tocopherol protects against a reduction in adenosylcobalamin in oxidatively stressed human cells.
    The Journal of nutrition, 1993, Volume: 123, Issue:7

    Excretion of methylmalonic acid by vitamin E-deficient patients and decreased labeling of adenosylcobalamin (AdoCbl) from cyanocobalamin in vitamin E-deficient rats suggest an interaction of vitamins E and B-12. We studied this interaction in two human cell culture systems: foreskin fibroblasts and a hepatoma cell line (HepG2). We measured radiolabeling of AdoCbl and methylcobalamin from [57Co]hydroxycobalamin for 6 d in the presence and absence of linoleate (an oxidative stressor) and alpha-tocopherol. In both cell types, labeling of AdoCbl was lower in the presence of linoleate unless alpha-tocopherol was present. The decrease was accentuated by peroxidized linoleic acid; AdoCbl synthetic rate was inversely associated with thiobarbituric acid-reactive compound concentration. Subcellular partitioning of labeled cobalamin revealed less in mitochondria in the linoleate-stressed cells that were not treated with alpha-tocopherol. We conclude that lipoperoxidation reduces mitochondrial AdoCbl formation and that alpha-tocopherol exerts a protective effect in oxidatively stressed cells. We suggest that this subcellular deficiency in AdoCbl may be one mechanism by which vitamin E deficiency leads to neurologic injury. The mechanism seems primarily to involve an alteration in intracellular cobalamin distribution with perhaps a minor effect upon enzymes of AdoCbl synthesis.

    Topics: Carcinoma, Hepatocellular; Cells, Cultured; Cobamides; Fibroblasts; Humans; Linoleic Acid; Linoleic Acids; Lipid Peroxidation; Liver Neoplasms; Oxidation-Reduction; Tumor Cells, Cultured; Vitamin E

1993