linoleic-acid and 6-methoxy-2-naphthylacetic-acid

The photobiological properties of 6-methoxy-2-naphthylacetic acid (6-MNAA) were studied using a variety of in vitro phototoxicity assays: photohemolysis, photoperoxidation of linoleic acid, photosensitized degradation of histidine and thymine and the Candida phototoxicity test. 6-MNAA was phototoxic in vitro. 6-MNAA reduced nitro blue tetrazolium (NBT) when irradiated with lambda > or = 300 nm in deoxygenated aqueous buffer solution (pH 7.4). NBT can be reduced by reaction with the excited state of 6-MNAA subject to interference with molecular oxygen. The photohemolysis rate was inhibited by the presence of 1,4-diazabicyclo[2.2.2]octane (DABCO), sodium azide (NaN3) and reduced glutathione (GSH). Photoperoxidation of linoleic acid and photosensitized degradation of histidine and thymine were significantly inhibited by sodium azide and reduced glutathione. 6-MNAA was phototoxic to C. albicans, C. lipolytica and C. tropicalis. A mechanism involving singlet oxygen, radicals, and electron transfer reactions is suggested for the observed phototoxicity.

Topics: Aerobiosis; Anaerobiosis; Anti-Inflammatory Agents, Non-Steroidal; Butanones; Candida; Dermatitis, Phototoxic; DNA; Erythrocytes; Hemolysis; Histidine; Humans; In Vitro Techniques; Light; Linoleic Acid; Lipid Peroxidation; Nabumetone; Naphthaleneacetic Acids; Nitroblue Tetrazolium; Photochemistry; Photosensitizing Agents; Thymine