linarin and naringin

Differentiation of flavonoid congeners remains a challenging analytical problem and confirming the structures of the different isomers is difficult, even when they can be adequately separated from mixtures. In the present report, in order to overcome the limits of our recently proposed method that relies on the distinctive CID behaviors of [(flavonoid - H(+)) + Cu(2+)] complexes to obtain direct structural evidences, we decided to investigate the possibility of using Pb(II) complexation to generate significant differences upon CID. We selected five flavonoid diglycosides with targeted structural features to estimate the applicability of this methodology. Electrospray ionization from methanol-not acetonitrile-solutions was advantageously used for preparing the [(flavonoid diglycoside - H(+)) + Pb(2)+](+) complexes. Upon collisional activation, [(flavonoid diglycoside- H(+)) + Pb(2+)](+) ions mainly dissociate by glycosidic bond cleavage. Nevertheless, specific cross-ring cleavages are also induced and lead to a clear-cut determination of (i) the nature of the disaccharide group, i.e. rutinose or neohesperidose, (ii) the nature of the aglycone part, flavanone or flavone and (iii) the relative position of the disaccharide substituent on the aglycone part, i.e. 3-O- vs 7-O positions.

Topics: Disaccharides; Flavanones; Flavones; Flavonoids; Glycosides; Hesperidin; Lead; Methanol; Molecular Structure; Rutin; Solutions; Spectrometry, Mass, Electrospray Ionization

To develop a HPLC method for the simultaneous determination of chlorogenic acid, cryptochlorogenic acid, caffeic acid, naringin, hesperidin and linarin in xiao' erjinning oral liquid.. The chromatographic separation was achieved on a Lichrospher C18 (4.6 mm x 250 mm, 5 microm) column with a mobile phase which was composed of acetonitrile(A) and 0.4% phosphoric acid(B) for gradient elution (10:90-18:82-27:73). The flow rate was (0.8-1.1-0.8) mL x min(-1), the column temperature was 30 degrees C and the detection wavelength was set at 300 nm.. The results showed that 6 effective components were separated well and showed good linearity. The average recoveries were between 95%-105%.. The method is proved to be rapid, accurate, credible and repeatable. It can be used for the quality control of Xiao'erjinning oral liquid.

Topics: Caffeic Acids; Chlorogenic Acid; Chromatography, High Pressure Liquid; Dosage Forms; Drugs, Chinese Herbal; Flavanones; Glycosides; Hesperidin