likviriton and nodakenin

A simple, convenient, rapid and accurate high-performance liquid chromatographic (HPLC) method was established for the simultaneous determination about three ingredients of a traditional herbal prescription, Guibi-tang (GBT): liquiritin (1), nodakenin (2) and glycyrrhizin (3). Chromatographic analysis on the three components was separated within 35 min on a Gemini C18 column and maintained at 40°C. The mobile phase consisted of water with 1.0% (v/v) acetic acid (solvent A) and acetonitrile with 1.0% (v/v) acetic acid (solvent B) in gradient mode at a flow-rate of 1.0 mL/min. Chromatograms were acquired at 254, 280 and 330 nm in a photodiode array (PDA) detector. The calibration curves showed excellent linearity (R(2)=1.0000). The average recovery of three compounds was >93.5%, with a relative standard deviation (RSD) of <2.0%. The intra-day and inter-day precision (RSD) of the three components were 0.04%-3.04% and 0.11%-2.48%, respectively. The contents of compounds 1-3 in GBT were 1.09-1.10, 2.35-2.37 and 0.81-0.82 mg/g, respectively.

Topics: Calibration; Chromatography, High Pressure Liquid; Coumarins; Drugs, Chinese Herbal; Flavanones; Glucosides; Glycyrrhizic Acid; Limit of Detection

A simple and accurate high-performance liquid chromatographic method was applied to the quantitative analysis of seven components of the traditional herbal prescription Jaeumganghwa-tang (JGT), including 5-hydroxymethyl-2-furaldehyde, albiflorin, paeoniflorin, liquiritin, ferulic acid, nodakenin, and glycyrrhizin. All seven compounds were separated in less than 40 min on a Gemini C18 column at 40°C by gradient elution using 1.0% (v/v) aqueous acetic acid and acetonitrile containing 1.0% (v/v) acetic acid as mobile phase. The flow rate was 1.0 mL/min and the detector was a photodiode array (PDA) set at 230 nm, 254 nm, 280 nm, and 330 nm. The calibration curves showed good linearity (r (2) > 0.9998) in different concentration ranges. The recovery of each component was in the range of 91.47-102.62%, with relative standard deviations (RSDs, %) less than 4.5%. The RSDs (%) for intra- and interday precision were 0.06-2.85% and 0.06-2.83%, respectively. The concentrations of the seven components in JGT were in the range 0.74-5.48 mg/g.

Topics: Benzoates; Bridged-Ring Compounds; Chromatography, High Pressure Liquid; Coumaric Acids; Coumarins; Drugs, Chinese Herbal; Flavanones; Furaldehyde; Glucosides; Glycyrrhizic Acid; Magnoliopsida; Monoterpenes

A high-performance liquid chromatographic (HPLC) method was developed for quantitative analysis of seven components, 5-hydroxymethyl-2-furaldehyde (1), albiflorin (2), paeoniflorin (3), liquiritin (4), ferulic acid (5), nodakenin (6), and glycyrrhizin (7) of Palmul-tang (PMT), a traditional Korean medicine. HPLC analysis was performed using a Gemini C18 column at 40°C, and photodiode array (PDA) detection at 230 nm, 254 nm, 280 nm, 320 nm, and 330 nm was used for quantification of the seven components in PMT. The mobile phase was a gradient flow composed of two solvent systems. Solvent A was 1.0% (v/v) aqueous acetic acid and solvent B was acetonitrile containing 1.0% (v/v) acetic acid. Calibration curves were acquired with r (2) values > 0.9998, and the relative standard deviations (RSDs, %) for intra- and interday precision were both less than 6.0%. The recovery of each component was in the range of 90.66-103.79%, with a RSD less than 5.0%. The contents of the seven components in PMT range form 0.61-6.21 mg/g. Additionally, we investigated the cytotoxicity of the extract against the RBL-1 and BEAS-2B cell lines, as well as splenocytes.

Topics: Animals; Benzoates; Bridged-Ring Compounds; Cell Line, Tumor; Chemistry, Pharmaceutical; Chromatography, High Pressure Liquid; Coumaric Acids; Coumarins; Cytotoxins; Drug Evaluation, Preclinical; Flavanones; Furaldehyde; Glucosides; Glycyrrhizic Acid; Medicine, Korean Traditional; Mice; Mice, Inbred BALB C; Monoterpenes; Plant Preparations; Rats