lignans and asperuloside

To study the chemical constituents of water extracted fraction from Oldenlandia diffusa.. The compounds were isolated and purified by column chromatography on macroporous resin,silica gel,MCI gel,Sephadex LH-20,ODS medium pressure liquid chromatography and RP-semi-preparative HPLC. The structures of compounds were elucidated on the basis of physicochemical and spectral analysis.. 16 compounds were isolated from the water extract of Oldenlandia diffusa,and their structures were identified as asperuloside( 1),deacetyl asperuloside( 2),geniposide( 3),10-dehydrogeniposide( 4),daphylloside( 5),diffusoside A( 6),diffusoside B( 7),coniferin( 8),scandoside methyl ester( 9),acetyl scandoside methyl ester( 10),deacetylasperulosidic acid methyl ester( 11),gardenoside( 12),galioside( 13),galioside 10-acetate( 14),loliolide( 15) and( +)-neo-olivil( 16),respectively.. Compounds 3,8 and 14 ~ 16 are obtained from Oldenlandia diffusa for the first time.

Topics: Chromatography, High Pressure Liquid; Cyclopentane Monoterpenes; Glucosides; Iridoids; Lignans; Oldenlandia; Pyrans

To investigate the chemical constituents from the root of Lasianthus acuminatissimus of Rubiaceae.. Normal, reverse phase silica gel, macroporous resin column and HPLC chromatography were used for isolation. Spectroscopic methods (13C-NMR, 1H-NMR, DEPT, EI-MS) were used for identification.. Six compounds were isolated and elucidated as isoscopletin(I), isoscopletin-6-O-beta-D-glucopyranoside (II), beta configuration of Mudanoside-A (Ill), asperuloside (IV), (-)-pinoresinol 4-O-beta-D-glucopyranoside (V), daucosterol (VI).. These compounds were obtained from this plant for the first time.

Topics: Cyclopentane Monoterpenes; Glucosides; Glycosides; Lignans; Plant Roots; Plants, Medicinal; Pyrans; Rubiaceae; Sitosterols