licoflavone-c and derrone

Ten compounds were isolated and purified from cell suspension cultures of Cudrania tricuspidata with silica-gel column chromatography, semi-preparative HPLC and Sephadex LH-20. On the base of their physicochemical properties and spectral data, their structures were identified as 1, 3, 5-trihydroxy4-(3, 3-dimethylallyl) xanthone (1), wighteone (2), 6-prenylapigenin (3), licoflavone C(4), cudraflavanone C(5), erythrivarone A (6), derrone (7), carthamidin (8), genistein (9) and aromadendrin (10). Among them, compounds 2-10 were flavonoids, and compound 1 was a xanthone which was isolated from the plant for the first time.

Topics: Cell Culture Techniques; Chromatography, High Pressure Liquid; Flavanones; Flavones; Flavonoids; Genistein; Moraceae; Plant Leaves; Plants, Medicinal; Xanthones

We have investigated the antibacterial, antifungal and cytotoxic activities of two flavonoids isolated from Retama raetam flowers using the disc diffusion and micro-dilution broth methods. The cytotoxic activity was tested against Hep-2 cells using the MTT assay. The compounds licoflavone C (1) and derrone (2) were active against Pseudomonas aeruginosa and Escherichia coli (7.81-15.62 μg/mL) and showed important antifungal activity. Strong antifungal activity against Candida species (7.81 μg/mL) was for example found with compound 2. The tested compounds also showed strong cytotoxicity against Hep-2 cells. These two compounds may be interesting antimicrobial agents to be used against infectious diseases caused by many pathogens.

Topics: Anti-Bacterial Agents; Antifungal Agents; Bacteria; Candida; Fabaceae; Flavones; Flavonoids; Flowers; Hep G2 Cells; Humans; Microbial Sensitivity Tests; Plant Extracts