leupeptins and benzylamine

leupeptins has been researched along with benzylamine* in 1 studies

Other Studies

1 other study(ies) available for leupeptins and benzylamine

Article	Year
Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands. Angewandte Chemie (International ed. in English), 2015, Apr-13, Volume: 54, Issue:16 Fluorine-19 NMR and hyperpolarization form a powerful combination for drug screening. Under a competitive equilibrium with a selected fluorinated reporter ligand, the dissociation constant (K(D)) of other ligands of interest is measurable using a single-scan Carr-Purcell-Meiboom-Gill (CPMG) experiment, without the need for a titration. This method is demonstrated by characterizing the binding of three ligands with different affinities for the serine protease trypsin. Monte Carlo simulations show that the highest accuracy is obtained when about one-half of the bound reporter ligand is displaced in the binding competition. Such conditions can be achieved over a wide range of affinities, allowing for rapid screening of non-fluorinated compounds when a single fluorinated ligand for the binding pocket of interest is known. Topics: Benzamidines; Benzylamines; Binding, Competitive; Fluorine; Kinetics; Leupeptins; Ligands; Magnetic Resonance Spectroscopy; Monte Carlo Method; Trypsin	2015

Article

Year

Affinity screening using competitive binding with fluorine-19 hyperpolarized ligands.

Angewandte Chemie (International ed. in English), 2015, Apr-13, Volume: 54, Issue:16

Fluorine-19 NMR and hyperpolarization form a powerful combination for drug screening. Under a competitive equilibrium with a selected fluorinated reporter ligand, the dissociation constant (K(D)) of other ligands of interest is measurable using a single-scan Carr-Purcell-Meiboom-Gill (CPMG) experiment, without the need for a titration. This method is demonstrated by characterizing the binding of three ligands with different affinities for the serine protease trypsin. Monte Carlo simulations show that the highest accuracy is obtained when about one-half of the bound reporter ligand is displaced in the binding competition. Such conditions can be achieved over a wide range of affinities, allowing for rapid screening of non-fluorinated compounds when a single fluorinated ligand for the binding pocket of interest is known.

Topics: Benzamidines; Benzylamines; Binding, Competitive; Fluorine; Kinetics; Leupeptins; Ligands; Magnetic Resonance Spectroscopy; Monte Carlo Method; Trypsin

2015