leukotriene-b4 and anisodamine

The effects of leukotrienes (LTs) and platelet activating factor (PAF) on DNA synthesis in bovine anterior cerebral arterial smooth muscle cells were studied. The results showed that LTB4, LTD4 and PAF were all stimulatory to the cells. The maximal rates of stimulation were 32%, 29% and 77%, respectively, for LTB4, LTD4 and PAF at 10(-7) mol/L. Both dauricine and anisodamine at concentrations of 10(-7) to 10(-4) mol/L exhibited strong inhibitory effects on LTs and PAF induced DNA synthesis.

Topics: Alkaloids; Animals; Benzylisoquinolines; Cattle; Cells, Cultured; Cerebral Arteries; DNA; Isoquinolines; Leukotriene B4; Muscle, Smooth, Vascular; Platelet Activating Factor; Platelet Aggregation Inhibitors; Solanaceous Alkaloids; SRS-A; Tetrahydroisoquinolines; Vasodilator Agents

[3H]Arachidonic acid (AA)-prelabeled mouse peritoneal macrophages were stimulated by calcium ionophore A-23187 to release [3H]AA metabolites. The major labeled products which were co-chromatographed with the authentic PG and LT standards by TLC and determined by liquid scintillation were 6-keto-PGF1 alpha, PGE2, LTC4, and LTB4. Anisodamine (Ani) significantly inhibited the A-23187-induced release of PG and LT from mouse macrophages in a dose-dependent manner. In the presence of Ani at 0.5 mmol/L, the A-23187-induced release of 6-keto-PGF1 alpha, PGE2, LTC4 and LTB4 was reduced by 57%, 20%, 53% and 49%, respectively. A-23187-induced release of 6-keto-PGF1 alpha measured by RIA from bovine aorta endothelial cells was also significantly inhibited by Ani in a dose-dependent manner. These results indicate that the calcium-antagonistic effects of Ani may not only play a significant role in its inhibiting release of PG and LT, but also contribute to its salutary effects in the treatment of septic shock.

Topics: 6-Ketoprostaglandin F1 alpha; Animals; Aorta; Calcimycin; Cattle; Cells, Cultured; Dose-Response Relationship, Drug; Endothelium, Vascular; Female; Leukotriene B4; Macrophages; Male; Mice; Peritoneal Cavity; Prostaglandins; Prostaglandins E; Solanaceous Alkaloids; SRS-A

Topics: Animals; Arachidonic Acids; Fatty Acids, Unsaturated; Hydroxyeicosatetraenoic Acids; Leukotriene B4; Male; Neutrophils; Pleura; Rats; Rats, Inbred Strains; Solanaceous Alkaloids; Thromboxane B2