leptin and plumbagin

To investigate the effect of plumbagin on the expression of TNF-alpha and PDGF-BB in human hepatic stellate cells (HSC-LX2) activated by Leptin.. HSC-LX2 were cultured in vitro and stimulated by Leptin for 24 hours then treated with different concentrations of plumbagin for 24 hours, the expressions of TNF-alpha mRNA and PDGF-BB mRNA were determined by Realtime quantitative PCR, the protein expressions of TNF-alpha and PDGF-BB were determined-by Western blotting.. The expressions of TNF-alpha mRNA and PDGF-BB mRNA of treatment groups were significantly reduced, especially in high dose group (P < 0.01), and Western blotting analyses revealed similar trends in protein expression.. Plumbagin may prevent the formation of hepatic fibrosis and its mechanism may be related to decreasing the level of mRNA of TNF-alpha and PDGF-BB and the protein of PDGF-BB.

Topics: Becaplermin; Cells, Cultured; Gene Expression Regulation; Hepatic Stellate Cells; Humans; Leptin; Liver Cirrhosis; Naphthoquinones; Plumbaginaceae; Polymerase Chain Reaction; Proto-Oncogene Proteins c-sis; RNA, Messenger; Tumor Necrosis Factor-alpha