leptin and carvacrol

Natural products are popular insights for researchers to investigate promising anti-cancer agents since some of these substances have lesser adverse effects restricting the treatment than traditional chemotherapeutic agents. A well-known monoterpene Carvacrol, widely consumed in Mediterranean cuisine and lower risks of cancer, has efficient anticancer effects. However, the mechanism of action is yet to be discovered.. The investigation aims to illuminate a new perceptive in the role of this substance on colorectal cancer treatment, by the means of differences in a well-defined range of soluble factors. Carvacrol effect on both HT-29 and HCT-116 cell lines was evaluated on proliferation and the IC50 values were calculated by the RTCA xCELLigence device. Then MAGPIX assay was performed to obtain the changes in soluble factors of the cell lines.. The Multiplexing assay suggests some of these factors were altered in favor of surviving and proliferation in aggressive cell line HCT-116 whereas they were altered against these characters in HT-29, were correlated with the increased IC50 concentration of HCT- 116 in carvacrol treatment.. The current study indicates that differences in the levels of these soluble factors could modulate the anticancer effect related to carvacrol.

Topics: Cell Proliferation; Colorectal Neoplasms; Cymenes; Granulocyte-Macrophage Colony-Stimulating Factor; HCT116 Cells; HT29 Cells; Humans; Leptin; Prolactin; Transforming Growth Factor alpha; Vascular Endothelial Growth Factor Receptor-2

The present investigation was designed to examine the possible additive hypolipidemic effect of carvacrol (CARV) in combination with simvastatin (SIM) on poloxamer 407 (P407)-induced hyperlipidemia. Rats were injected with P407, (500 mg/ kg; i.p.), twice a week, for 30 days. Treatment was carried out by administration of SIM (20 mg/kg/day; p.o.) or CARV (50 mg/kg/day; p.o.) or combination of them. Treatment with CARV significantly decreased total cholesterol, triglycerides, low-density lipoprotein, atherogenic index, leptin, and increased high-density lipoprotein and adiponectin. Moreover, CARV potentiated the hypolipidemic effect of SIM. Both SIM and CARV alleviated the oxidative stress induced by P407. Interestingly, CARV, when combined with SIM, significantly ameliorated SIM-induced liver and muscle injury by reducing the level of alanine aminotransferase, aspartate aminotransferase, lactate dehydrogenase, creatine kinase, and myoglobin and restoring the normal histological picture of both liver and muscle as well as apoptosis.

Topics: Adiponectin; Animals; Anticholesteremic Agents; Caspase 3; Catalase; Cholesterol; Cymenes; Drug Combinations; Drug Evaluation, Preclinical; Drug Synergism; Glutathione; Hypercholesterolemia; Leptin; Liver; Male; Monoterpenes; Muscle, Skeletal; Organ Size; Rats, Sprague-Dawley; Simvastatin; Triglycerides