lactosyl-lysosphingolipid has been researched along with 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene* in 1 studies
1 other study(ies) available for lactosyl-lysosphingolipid and 4-4-difluoro-4-bora-3a-4a-diaza-s-indacene
| Article | Year |
|---|---|
Evaluation of reversed-phase nano liquid chromatography conditions by using reversed-phase thin layer chromatography based on Hansen solubility parameters for the analysis of amphiphilic glycosylsphingolipid transformations.
Pulse chase analysis is often used in investigating dynamics of cellular substances. Fluorescently labeled lactosyl sphingosine molecule is useful in chasing its transformation, however the analysis of such metabolites in attomole level is of extreme difficult due to the presence of large amount of endogenous amphiphilic molecules such as glycosphingolipids, sphingomyerin, and glycerophospholipids. Nano LC suites for analyzing the attomole scale metabolites, therefore removal of endogenous substances prior to nano LC and finding appropriate nano LC conditions are necessary. Thus, we focused on the solubility of fluorescent BODIPY-labeled lactosylsphingosine (Lac-Sph-BODIPY) to identify suitable solvents to remove endogenous compounds. In this study, we evaluated solvents by using C18 thin layer chromatography (RP TLC). The mobility (R Topics: Animals; Boron Compounds; CHO Cells; Chromatography, Reverse-Phase; Chromatography, Thin Layer; Cricetinae; Cricetulus; Hydrogen Bonding; Nanotechnology; Psychosine; Silicon Dioxide; Solubility; Solvents; Sphingolipids; Zirconium | 2018 |