Page last updated: 2024-08-17

lactose and isopropyl thiogalactoside

lactose has been researched along with isopropyl thiogalactoside in 58 studies

Research

Studies (58)

TimeframeStudies, this research(%)All Research%
pre-199011 (18.97)18.7374
1990's13 (22.41)18.2507
2000's18 (31.03)29.6817
2010's12 (20.69)24.3611
2020's4 (6.90)2.80

Authors

AuthorsStudies
Goldschmidt, R; Hidalgo, C; Reyes, J1
Friedman, BE; Matthews, KS; Olson, JS2
Burgum, AA; Friedman, BE; Matthews, KS; Sams, CF; Yang, DS1
Matthews, KS; O'Gorman, RB2
Huber, RE; Turner, CL1
Brochon, JC; Charlier, M; Hélène, C; Maurizot, JC; Wahl, P1
Coynault, C; Guiso, N; Le Minor, L1
Boichenko, MN; Maisky, AI1
Brooker, RJ1
Brand, JM; Gray, JS; Lindner, WA; Mildenhall, JP1
Hasan, N; Szybalski, W1
Frillingos, S; Kaback, HR; Voss, J; Wu, J1
Saier, MH; Ye, JJ1
Okazaki, N; Tsuchiya, T; Tsuda, M; Wilson, TH1
Dyr, JE; Hirsch, I; Liska, V; Suttnar, J; Vonka, V1
Ho, YR; Hsueh, HC; Huang, WT; Lai, MD; Leung, HW; Lin, MT; Liu, HS; Wu, JD1
Hansen, LH; Knudsen, S; Sørensen, SJ1
Chiou, CY; Kao, HS; Shaw, GC1
Broome-Smith, JK; Hashemzadeh-Bonehi, L; Hennessey, ES; Jacob, JP; Mehraein-Ghomi, F; Mitsopoulos, C1
Arents, JC; Bader, R; Hogema, BM; Postma, PW1
Gosink, M; Liu, JY; Miller, PF; Olson, ER1
Barry, JK; Maki, AH; Matthews, KS; Ozarowski, A1
Condemine, G1
Gerk, LP; Leven, O; Müller-Hill, B1
Donovan, RS; Glick, BR; Robinson, CW1
Koh, I; Lee, J; Park, YH; Rhee, SK; Saraswat, V; Song, KB1
Cupp-Vickery, JR; Woyski, D1
Mills, AA1
Kuhn, B; Kulbe, KD; Nidetzky, B; Petzelbauer, I; Splechtna, B1
Fairbanks, BM; Maheshwari, A; Matthews, KS; Swint-Kruse, L; Zhan, H1
Iannaccone, A; Jablonski, MM; Wang, X1
Studier, FW1
Jianping, L; Peilin, C; Xiaoxia, L1
Ma, X; Ma, Y; Wang, T; Wei, D; Zheng, W1
Chen, QX; He, GQ; Li, CL1
Chou, HH; Keasling, JD; Lee, SK; Newman, JD; Pfleger, BF; Yoshikuni, Y1
Langley, DB; Matthews, KS; Swint-Kruse, L; Taraban, M; Trewhella, J; Whitten, AE; Zhan, H1
Cailliez-Grimal, C; Gelhaye, E; Jasniewski, J; Revol-Junelles, AM1
Baldwin, SA; Deacon, SE; Henderson, PJ; Knox, JP; McPherson, MJ; Phillips, SE; Postis, VL; Roach, PC; Wright, GS; Xia, X1
Khan, MA; Sadaf, S; Sajjad, M; Waheed Akhtar, M1
Browne, NK; Dockrell, M; Hashmi, P; Huang, Z; Price, RG1
Hatti-Kaul, R; Mamo, G; Mattiasson, B; Tran, TT1
Ding, Q; Ou, L; Wei, D; Wei, X1
Malakar, P; Venkatesh, KV1
Eames, M; Kortemme, T1
Duan, X; Wu, J; Zou, C1
Aviziotis, IG; Boudouvis, AG; Kavousanakis, ME1
Egger, E; Grabherr, R; Heinl, S; Heiss, S; Hörmann, A; Sonnleitner, M; Tauer, C1
Beitle, R; Fruchtl, M; Sakon, J1
Duan, X; Ran, H; Wu, D; Wu, J1
Eggenreich, B; Herwig, C; Spadiut, O; Ulonska, S; Veiter, L; Wurm, DJ1
Halbwirth, H; Hausjell, J; Molitor, C; Spadiut, O; Weissensteiner, J1
Bagheri, M; Khani, MH1
Han, JH; Kim, TH; Myung, SH; Park, J1
Bezerra, SGS; de Souza, TC; Gonçalves, LRB; Hissa, DC; Mammarella, EJ; Manzo, RM; Oliveira, RC1
Cheng, F; Wang, LY; Wu, ZS; Xi, ZJ; Xu, JM; Xue, YP; Zhao, KJ; Zheng, YG1

Reviews

1 review(s) available for lactose and isopropyl thiogalactoside

ArticleYear
Changing colors in mice: an inducible system that delivers.
    Genes & development, 2001, Jun-15, Volume: 15, Issue:12

    Topics: Animals; Gene Expression Regulation; Hair Color; Humans; Isopropyl Thiogalactoside; Lac Operon; Lactose; Mice; Mice, Mutant Strains; Monophenol Monooxygenase

2001

Other Studies

57 other study(ies) available for lactose and isopropyl thiogalactoside

ArticleYear
Induction and general properties of beta-galactosidase and beta-galactoside permease in Pseudomonas BAL-31.
    Journal of bacteriology, 1977, Volume: 129, Issue:2

    Topics: Carbohydrate Metabolism; Cell-Free System; Enzyme Induction; Galactosidases; Hydrogen-Ion Concentration; Hydrolysis; Isopropyl Thiogalactoside; Lactose; Membrane Transport Proteins; Nitrophenylgalactosides; Pseudomonas; Seawater; Temperature; Water Microbiology

1977
Interaction of lac repressor with inducer, kinetic and equilibrium measurements.
    Journal of molecular biology, 1977, Mar-25, Volume: 111, Issue:1

    Topics: Bacterial Proteins; DNA; Escherichia coli; Genes, Regulator; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Kinetics; Lactose; Operon; Osmolar Concentration; Protein Conformation; Thioglycosides

1977
Spectral studies of lactose repressor protein modified with nitrophenol reporter groups.
    The Journal of biological chemistry, 1977, May-25, Volume: 252, Issue:10

    Topics: Bacterial Proteins; Chloromercurinitrophenols; Enzyme Induction; Enzyme Repression; Glycerol; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Kinetics; Lactose; Ligands; Nitrophenylgalactosides; Operon; Phenylmercury Compounds; Protein Conformation; Spectrum Analysis

1977
N-Bromosuccinimide modification of Lac repressor protein.
    The Journal of biological chemistry, 1977, Jun-10, Volume: 252, Issue:11

    Topics: Amino Acids; Bacterial Proteins; Bromosuccinimide; Circular Dichroism; Cysteine; DNA; Escherichia coli; Fucose; Genes, Regulator; Glycosides; Isopropyl Thiogalactoside; Lactose; Molecular Weight; Oxidation-Reduction; Peptide Fragments; Protein Conformation; Succinimides; Tryptophan

1977
Differential binding of allolactose anomers to the lactose repressor of Escherichia coli.
    Journal of molecular biology, 1977, Sep-15, Volume: 115, Issue:2

    Topics: Binding, Competitive; Escherichia coli; Genes, Regulator; Isopropyl Thiogalactoside; Lactose; Operon; Stereoisomerism

1977
Time resolved spectroscopy of the tryptophyl fluorescence of the E. coli LAC repressor.
    Biochemical and biophysical research communications, 1977, Dec-21, Volume: 79, Issue:4

    Topics: Amino Acid Sequence; Bacterial Proteins; Enzyme Repression; Escherichia coli; Genes, Regulator; Isopropyl Thiogalactoside; Kinetics; Lactose; Operon; Spectrometry, Fluorescence; Tryptophan

1977
[Positivity of ONPG test versus the presence of beta-galactosidase in Enterobacteriaceae and other Gram-negative bacilli (author's transl)].
    Annales de microbiologie, 1977, Volume: 128B, Issue:1

    Topics: Bacteria; beta-Galactosidase; Enterobacteriaceae; Fermentation; Galactosidases; Glycosides; Isopropyl Thiogalactoside; Lactose; Nitrophenylgalactosides

1977
Kinetic studies of inducer binding to lactose repressor protein.
    The Journal of biological chemistry, 1976, Feb-25, Volume: 251, Issue:4

    Topics: Bacterial Proteins; Binding Sites; Enzyme Induction; Enzyme Repression; Escherichia coli; Genes; Isopropyl Thiogalactoside; Kinetics; Lactose; Mercury; Organometallic Compounds; Protein Binding; Thioglycosides

1976
Inducing effect of cortisone and insulin on the activity of beta-galactosidase in the E. coli strains K12.
    Journal of hygiene, epidemiology, microbiology, and immunology, 1976, Volume: 20, Issue:4

    Topics: Cortisone; Enzyme Induction; Escherichia coli; Galactosidases; Insulin; Isopropyl Thiogalactoside; Lactose; Mutation; Operon; Temperature

1976
Fluorescence and ultraviolet spectral studies of Lac repressor modified with N-bromosuccinimide.
    The Journal of biological chemistry, 1977, Jun-10, Volume: 252, Issue:11

    Topics: Bacterial Proteins; Bromosuccinimide; Genes, Regulator; Isopropyl Thiogalactoside; Lactose; Oxidation-Reduction; Scattering, Radiation; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Succinimides; Tryptophan

1977
An analysis of lactose permease "sugar specificity" mutations which also affect the coupling between proton and lactose transport. I. Val177 and Val177/Asn319 permeases facilitate proton uniport and sugar uniport.
    The Journal of biological chemistry, 1991, Mar-05, Volume: 266, Issue:7

    Topics: Biological Transport; Escherichia coli; Escherichia coli Proteins; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Lactose; Membrane Transport Proteins; Methylgalactosides; Monosaccharide Transport Proteins; Recombinant Proteins; Structure-Activity Relationship; Substrate Specificity; Symporters; Thiogalactosides

1991
Lactose and melibiose metabolism in Erwinia chrysanthemi.
    Journal of bacteriology, 1986, Volume: 168, Issue:2

    Topics: Acetyltransferases; alpha-Galactosidase; beta-Galactosidase; Biological Transport; Disaccharides; Enzyme Induction; Erwinia; Isopropyl Thiogalactoside; Lactose; Melibiose; Methylgalactosides; Mutation; Raffinose; Thiogalactosides

1986
Construction of lacIts and lacIqts expression plasmids and evaluation of the thermosensitive lac repressor.
    Gene, 1995, Sep-22, Volume: 163, Issue:1

    Topics: Amino Acid Sequence; Bacterial Proteins; beta-Galactosidase; Cloning, Molecular; Enzyme Induction; Escherichia coli; Escherichia coli Proteins; Gene Expression; Isopropyl Thiogalactoside; Lac Repressors; Lactose; Molecular Sequence Data; Plasmids; Point Mutation; Recombinant Proteins; Repressor Proteins; Restriction Mapping; Temperature

1995
Ligand-induced conformational changes in the lactose permease of Escherichia coli: evidence for two binding sites.
    Protein science : a publication of the Protein Society, 1994, Volume: 3, Issue:12

    Topics: Amino Acid Sequence; Anilino Naphthalenesulfonates; Bacterial Proteins; Binding Sites; Coumarins; Cysteine; Electron Spin Resonance Spectroscopy; Escherichia coli; Escherichia coli Proteins; Ethylmaleimide; Isopropyl Thiogalactoside; Lactose; Ligands; Membrane Transport Modulators; Membrane Transport Proteins; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutagenesis, Site-Directed; Protein Conformation; Recombinant Fusion Proteins; Spectrometry, Fluorescence; Spin Labels; Symporters; Thiogalactosides; Valine

1994
Cooperative binding of lactose and the phosphorylated phosphocarrier protein HPr(Ser-P) to the lactose/H+ symport permease of Lactobacillus brevis.
    Proceedings of the National Academy of Sciences of the United States of America, 1995, Jan-17, Volume: 92, Issue:2

    Topics: Allosteric Regulation; Bacterial Proteins; Binding, Competitive; Biological Transport; Carrier Proteins; Escherichia coli Proteins; Ethylmaleimide; Iodine Radioisotopes; Isopropyl Thiogalactoside; Isotope Labeling; Lactobacillus; Lactose; Melibiose; Membranes; Monosaccharide Transport Proteins; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Phosphoserine; Protein Binding; Symporters; Thiogalactosides

1995
Characterization of the lactose transport system in Citrobacter freundii.
    Biological & pharmaceutical bulletin, 1994, Volume: 17, Issue:6

    Topics: beta-Galactosidase; Citrobacter freundii; Enzyme Induction; Isopropyl Thiogalactoside; Lactose; Methylgalactosides; Substrate Specificity; Thiogalactosides

1994
Production and simple purification of a protein encoded by part of the gag gene of HIV-1 in the Escherichia coli HB101F+ expression system inducible by lactose and isopropyl-beta-D-thiogalactopyranoside.
    Journal of chromatography. B, Biomedical applications, 1994, Jun-03, Volume: 656, Issue:1

    Topics: Antibodies, Bacterial; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Gene Expression Regulation, Viral; Gene Products, gag; Genes, Viral; HIV-1; Humans; Isopropyl Thiogalactoside; Lactose; Recombinant Proteins

1994
The inducible lactose operator-repressor system is functional in the whole animal.
    DNA and cell biology, 1997, Volume: 16, Issue:1

    Topics: Animals; Bacterial Proteins; Cell Line; Escherichia coli Proteins; Gene Expression Regulation; Genes, ras; Humans; Isopropyl Thiogalactoside; Lac Operon; Lac Repressors; Lactose; Mice; Mice, Inbred BALB C; Neoplasms, Experimental; Repressor Proteins; Transfection

1997
The effect of the lacY gene on the induction of IPTG inducible promoters, studied in Escherichia coli and Pseudomonas fluorescens.
    Current microbiology, 1998, Volume: 36, Issue:6

    Topics: Escherichia coli; Escherichia coli Proteins; Genes, Bacterial; Isopropyl Thiogalactoside; Lactose; Membrane Transport Proteins; Monosaccharide Transport Proteins; Operon; Promoter Regions, Genetic; Pseudomonas fluorescens; Symporters

1998
Cloning, expression, and catabolite repression of a gene encoding beta-galactosidase of Bacillus megaterium ATCC 14581.
    Journal of bacteriology, 1998, Volume: 180, Issue:17

    Topics: Bacillus megaterium; Base Sequence; beta-Galactosidase; Cloning, Molecular; DNA Primers; Glucose; Isopropyl Thiogalactoside; Lactose; Mutation; Promoter Regions, Genetic; Recombinant Fusion Proteins; Transcription, Genetic

1998
Importance of using lac rather than ara promoter vectors for modulating the levels of toxic gene products in Escherichia coli.
    Molecular microbiology, 1998, Volume: 30, Issue:3

    Topics: Arabinose; Bacterial Proteins; Cloning, Molecular; Escherichia coli; Gene Expression; Genes, Bacterial; Isopropyl Thiogalactoside; Lactose; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Recombinant Proteins

1998
Autoregulation of lactose uptake through the LacY permease by enzyme IIAGlc of the PTS in Escherichia coli K-12.
    Molecular microbiology, 1999, Volume: 31, Issue:6

    Topics: beta-Galactosidase; Cyclic AMP; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli Proteins; Glucose; Glucose-6-Phosphate; Homeostasis; Isopropyl Thiogalactoside; Lactose; Membrane Transport Proteins; Monosaccharide Transport Proteins; Phosphoenolpyruvate Sugar Phosphotransferase System; Phosphorylation; Symporters; Time Factors

1999
The identification of a new family of sugar efflux pumps in Escherichia coli.
    Molecular microbiology, 1999, Volume: 31, Issue:6

    Topics: Antiporters; Bacterial Proteins; beta-Galactosidase; Dose-Response Relationship, Drug; Escherichia coli; Escherichia coli Proteins; Genomic Library; Immunoblotting; Isopropyl Thiogalactoside; Lactose; Monosaccharide Transport Proteins; Multigene Family; Plasmids; Time Factors; Yersinia pestis

1999
Ligand-induced conformational changes in lactose repressor: a phosphorescence and ODMR study of single-tryptophan mutants.
    Biochemistry, 1999, May-25, Volume: 38, Issue:21

    Topics: Amino Acid Substitution; Bacterial Proteins; Escherichia coli; Escherichia coli Proteins; Histidine; Isopropyl Thiogalactoside; Lac Repressors; Lactose; Ligands; Luminescent Measurements; Macromolecular Substances; Magnetic Resonance Spectroscopy; Models, Molecular; Mutagenesis, Site-Directed; Phenylalanine; Protein Conformation; Repressor Proteins; Tryptophan; Tyrosine

1999
Characterization of SotA and SotB, two Erwinia chrysanthemi proteins which modify isopropyl-beta-D-thiogalactopyranoside and lactose induction of the Escherichia coli lac promoter.
    Journal of bacteriology, 2000, Volume: 182, Issue:5

    Topics: Bacterial Outer Membrane Proteins; Bacterial Proteins; Biological Transport; Carbohydrate Metabolism; Dickeya chrysanthemi; Escherichia coli; Gene Expression Regulation, Bacterial; Genes, Bacterial; Isopropyl Thiogalactoside; Lac Operon; Lactose; Ligases; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutation; Polysaccharide-Lyases; Promoter Regions, Genetic; Recombinant Fusion Proteins; Transcription, Genetic

2000
Strengthening the dimerisation interface of Lac repressor increases its thermostability by 40 deg. C.
    Journal of molecular biology, 2000, Jun-09, Volume: 299, Issue:3

    Topics: Allosteric Regulation; Amino Acid Substitution; Bacterial Proteins; Binding Sites; Circular Dichroism; Codon, Terminator; Dimerization; DNA, Bacterial; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Lac Operon; Lac Repressors; Lactose; Lysine; Models, Molecular; Mutation; Operator Regions, Genetic; Protein Binding; Protein Conformation; Protein Denaturation; Protein Folding; Protein Renaturation; Repressor Proteins; Temperature; Thermodynamics

2000
Optimizing the expression of a monoclonal antibody fragment under the transcriptional control of the Escherichia coli lac promoter.
    Canadian journal of microbiology, 2000, Volume: 46, Issue:6

    Topics: Antibodies, Monoclonal; beta-Galactosidase; Blotting, Western; Culture Media; Escherichia coli; Glucose; Glycerol; Immunoglobulin Fab Fragments; Isopropyl Thiogalactoside; Lac Operon; Lactose; Promoter Regions, Genetic; Recombinant Proteins; Transcriptional Activation

2000
Secretory production of Arthrobacter levan fructotransferase from recombinant Escherichia coli.
    FEMS microbiology letters, 2001, Feb-20, Volume: 195, Issue:2

    Topics: Amino Acid Sequence; Arthrobacter; Bioreactors; Escherichia coli; Gene Expression; Genes, Bacterial; Hexosyltransferases; Isopropyl Thiogalactoside; Lactose; Molecular Sequence Data; Recombinant Fusion Proteins

2001
Enhanced expression of cytochrome P450s from lac-based plasmids using lactose as the inducer.
    Archives of biochemistry and biophysics, 2001, Apr-15, Volume: 388, Issue:2

    Topics: Cytochrome P-450 Enzyme System; Escherichia coli; Gene Expression Regulation, Enzymologic; Isopropyl Thiogalactoside; Lactose; Plasmids

2001
Hydrolysis of lactose by beta-glycosidase CelB from hyperthermophilic archaeon Pyrococcus furiosus: comparison of hollow-fiber membrane and packed-bed immobilized enzyme reactors for continuous processing of ultrahigh temperature-treated skim milk.
    Applied biochemistry and biotechnology, 2002,Spring, Volume: 98-100

    Topics: Animals; Bioreactors; Cellulase; Hydrolysis; Isopropyl Thiogalactoside; Kinetics; Lactose; Milk; Pyrococcus furiosus

2002
Perturbation from a distance: mutations that alter LacI function through long-range effects.
    Biochemistry, 2003, Dec-02, Volume: 42, Issue:47

    Topics: Amino Acid Substitution; Bacterial Proteins; DNA-Binding Proteins; Escherichia coli Proteins; Isopropyl Thiogalactoside; Lac Repressors; Lactose; Melibiose; Mutagenesis, Site-Directed; Operator Regions, Genetic; Phenotype; Protein Conformation; Protein Denaturation; Protein Structure, Tertiary; Repressor Proteins; Spectrometry, Fluorescence; Spectrophotometry, Ultraviolet; Urea

2003
Permissive glycan support of photoreceptor outer segment assembly occurs via a non-metabolic mechanism.
    Molecular vision, 2003, Dec-16, Volume: 9

    Topics: Animals; Autoradiography; Cell Differentiation; Cell Membrane; Culture Media; Dose-Response Relationship, Drug; Embryo, Nonmammalian; Galactose; Isopropyl Thiogalactoside; Lactose; Mannose; Organ Culture Techniques; Pigment Epithelium of Eye; Rod Cell Outer Segment; Xenopus laevis

2003
Protein production by auto-induction in high density shaking cultures.
    Protein expression and purification, 2005, Volume: 41, Issue:1

    Topics: Amino Acids; Bacteriological Techniques; Colony Count, Microbial; Culture Media; DNA-Directed RNA Polymerases; Energy Metabolism; Escherichia coli; Gene Expression; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Lactose; Phosphates; Recombinant Proteins; Viral Proteins

2005
Effect of inducers on the production of 5-aminolevulinic acid by recombinant Escherichia coli.
    Preparative biochemistry & biotechnology, 2006, Volume: 36, Issue:3

    Topics: 5-Aminolevulinate Synthetase; Aminolevulinic Acid; Enzyme Induction; Escherichia coli; Isopropyl Thiogalactoside; Lactose

2006
Optimization and high-level expression of a functional GST-tagged rHLT-B in Escherichia coli and GM1 binding ability of purified rHLT-B.
    Journal of microbiology (Seoul, Korea), 2006, Volume: 44, Issue:3

    Topics: Bacterial Toxins; Biotechnology; Culture Media; Enterotoxins; Enzyme-Linked Immunosorbent Assay; Escherichia coli; Escherichia coli Proteins; G(M1) Ganglioside; Glutathione Transferase; Isopropyl Thiogalactoside; Lactose; Recombinant Fusion Proteins

2006
[The influences of lactose as an inducer on expression of plant des-pGlu1-brazzein in Escherichia coli].
    Sheng wu gong cheng xue bao = Chinese journal of biotechnology, 2006, Volume: 22, Issue:6

    Topics: Dose-Response Relationship, Drug; Escherichia coli; Isopropyl Thiogalactoside; Lactose; Plant Proteins; Plasmids; Temperature; Time Factors; Up-Regulation

2006
Directed evolution of AraC for improved compatibility of arabinose- and lactose-inducible promoters.
    Applied and environmental microbiology, 2007, Volume: 73, Issue:18

    Topics: Arabinose; AraC Transcription Factor; Escherichia coli; Escherichia coli Proteins; Evolution, Molecular; Isopropyl Thiogalactoside; Lactose; Mutagenesis, Site-Directed; Operon; Promoter Regions, Genetic

2007
Ligand-induced conformational changes and conformational dynamics in the solution structure of the lactose repressor protein.
    Journal of molecular biology, 2008, Feb-15, Volume: 376, Issue:2

    Topics: Allosteric Regulation; Bacterial Proteins; Binding Sites; Dimerization; DNA; Isopropyl Thiogalactoside; Lactose; Ligands; Models, Biological; Models, Molecular; Molecular Weight; Protein Binding; Protein Conformation; Protein Structure, Secondary; Protein Structure, Tertiary; Repressor Proteins; Scattering, Small Angle; Thermodynamics

2008
Optimization of the production and purification processes of carnobacteriocins Cbn BM1 and Cbn B2 from Carnobacterium maltaromaticum CP5 by heterologous expression in Escherichia coli.
    Journal of microbiological methods, 2008, Volume: 73, Issue:1

    Topics: Amino Acid Sequence; Bacterial Proteins; Bacteriocins; Chromatography, Affinity; Culture Media; Escherichia coli; Fermentation; Gene Expression; Isopropyl Thiogalactoside; Lactobacillus; Lactose; Molecular Sequence Data; Protein Engineering; Recombinant Fusion Proteins; Thioredoxins

2008
Reliable scale-up of membrane protein over-expression by bacterial auto-induction: from microwell plates to pilot scale fermentations.
    Molecular membrane biology, 2008, Volume: 25, Issue:8

    Topics: Bioreactors; Crystallography, X-Ray; Culture Media; Fermentation; Glucose; Glycerol; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Lactose; Membrane Proteins; Oxygen; Pilot Projects; Recombinant Proteins; Transcriptional Activation

2008
Production enhancement and refolding of caprine growth hormone expressed in Escherichia coli.
    Protein expression and purification, 2009, Volume: 68, Issue:1

    Topics: Animals; Cell Line, Tumor; Cell Proliferation; Chromatography, Affinity; Culture Media; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Goats; Growth Hormone; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Lactose; Pilot Projects; Protein Folding; Rats; Recombinant Proteins; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

2009
Evaluation of new chromogenic substrates for the detection of coliforms.
    Journal of applied microbiology, 2010, Volume: 108, Issue:5

    Topics: Agar; Bacteria; beta-Galactosidase; Chromogenic Compounds; Culture Media; Enterobacteriaceae; Escherichia coli; Isopropyl Thiogalactoside; Lactose; Urine

2010
A thermostable phytase from Bacillus sp. MD2: cloning, expression and high-level production in Escherichia coli.
    Journal of industrial microbiology & biotechnology, 2010, Volume: 37, Issue:3

    Topics: 6-Phytase; Bacillus; Bacterial Proteins; Bioreactors; Calcium Chloride; Cloning, Molecular; Culture Media; Enzyme Activators; Enzyme Stability; Escherichia coli; Gene Expression; Hot Temperature; Isopropyl Thiogalactoside; Lactose; Protein Stability; Recombinant Proteins; Transcriptional Activation

2010
Optimum induction of recombinant thymidine phosphorylase and its application.
    Nucleosides, nucleotides & nucleic acids, 2011, Volume: 30, Issue:5

    Topics: Enzyme Activation; Enzyme Stability; Escherichia coli; Gene Expression; Gene Expression Regulation, Bacterial; Isopropyl Thiogalactoside; Lactose; Recombinant Proteins; Thymidine Phosphorylase; Time Factors

2011
Effect of substrate and IPTG concentrations on the burden to growth of Escherichia coli on glycerol due to the expression of Lac proteins.
    Applied microbiology and biotechnology, 2012, Volume: 93, Issue:6

    Topics: Escherichia coli; Gene Expression Regulation, Bacterial; Glycerol; Isopropyl Thiogalactoside; Lac Operon; Lactose

2012
Cost-benefit tradeoffs in engineered lac operons.
    Science (New York, N.Y.), 2012, May-18, Volume: 336, Issue:6083

    Topics: Base Sequence; beta-Galactosidase; Biocatalysis; Biological Transport; Escherichia coli; Escherichia coli Proteins; Gene Expression Regulation, Bacterial; Gene Knockout Techniques; Genetic Engineering; Isopropyl Thiogalactoside; Lac Operon; Lac Repressors; Lactose; Models, Biological; Molecular Sequence Data; Monosaccharide Transport Proteins; Mutation; Symporters

2012
Enhanced extracellular production of recombinant Bacillus deramificans pullulanase in Escherichia coli through induction mode optimization and a glycine feeding strategy.
    Bioresource technology, 2014, Volume: 172

    Topics: Bacillus; Cell Proliferation; Escherichia coli; Glycine; Glycoside Hydrolases; Isopropyl Thiogalactoside; Lactose

2014
Effect of Intrinsic Noise on the Phenotype of Cell Populations Featuring Solution Multiplicity: An Artificial lac Operon Network Paradigm.
    PloS one, 2015, Volume: 10, Issue:7

    Topics: Asymmetric Cell Division; Escherichia coli; Gene Regulatory Networks; Genetic Heterogeneity; Isopropyl Thiogalactoside; Lac Operon; Lactose; Models, Statistical; Phenotype; Stochastic Processes

2015
Evaluation of novel inducible promoter/repressor systems for recombinant protein expression in Lactobacillus plantarum.
    Microbial cell factories, 2016, Mar-10, Volume: 15

    Topics: Base Sequence; Blotting, Western; DNA-Directed RNA Polymerases; Isopropyl Thiogalactoside; Lactobacillus plantarum; Lactose; Molecular Sequence Data; Plasmids; Promoter Regions, Genetic; Recombinant Proteins; Repressor Proteins; Viral Proteins; Xylose

2016
Alternate carbohydrate and nontraditional inducer leads to increased productivity of a collagen binding domain fusion protein via fed-batch fermentation.
    Journal of biotechnology, 2016, May-20, Volume: 226

    Topics: Acetates; Batch Cell Culture Techniques; Biomass; Blotting, Western; Carbohydrates; Collagen; Fermentation; Glucose; Glycerol; Isopropyl Thiogalactoside; Lactose; Plasmids; Protein Domains; Recombinant Fusion Proteins; Time Factors

2016
Enhanced Production of Recombinant Thermobifida fusca Isoamylase in Escherichia coli MDS42.
    Applied biochemistry and biotechnology, 2016, Volume: 180, Issue:3

    Topics: Actinomycetales; Biomass; Bioreactors; Electrophoresis, Polyacrylamide Gel; Escherichia coli; Isoamylase; Isopropyl Thiogalactoside; Lactose; Recombinant Proteins; Temperature; Time Factors

2016
The E. coli pET expression system revisited-mechanistic correlation between glucose and lactose uptake.
    Applied microbiology and biotechnology, 2016, Volume: 100, Issue:20

    Topics: Escherichia coli; Gene Expression; Gene Expression Regulation; Genetic Vectors; Glucose; Isopropyl Thiogalactoside; Lactose; Recombinant Proteins; Single-Chain Antibodies; Transcriptional Activation

2016
E. coli HMS174(DE3) is a sustainable alternative to BL21(DE3).
    Microbial cell factories, 2018, Oct-30, Volume: 17, Issue:1

    Topics: Batch Cell Culture Techniques; Escherichia coli; Isopropyl Thiogalactoside; Lactose; Mixed Function Oxygenases

2018
Skimmed milk as an alternative for IPTG in induction of recombinant protein expression.
    Protein expression and purification, 2020, Volume: 170

    Topics: Animals; Bacterial Proteins; Cloning, Molecular; Escherichia coli; Flagellin; Gene Expression; Genetic Vectors; Histidine; Isopropyl Thiogalactoside; Lac Operon; Lactose; Milk; Oligopeptides; Recombinant Fusion Proteins; Salmonella typhimurium

2020
Development of the Mammalian Expression Vector System that can be Induced by IPTG and/or Lactose.
    Journal of microbiology and biotechnology, 2020, Aug-28, Volume: 30, Issue:8

    Topics: Animals; Gene Expression Regulation; HEK293 Cells; HeLa Cells; Humans; Isopropyl Thiogalactoside; Lac Operon; Lactose; Transfection

2020
Alternative Heterologous Expression of L-Arabinose Isomerase from Enterococcus faecium DBFIQ E36 By Residual Whey Lactose Induction.
    Molecular biotechnology, 2021, Volume: 63, Issue:4

    Topics: Aldose-Ketose Isomerases; Bacterial Proteins; Cloning, Molecular; Culture Media; Enterococcus faecium; Escherichia coli; Gene Expression Regulation, Bacterial; Gene Expression Regulation, Enzymologic; Glucose; Glycerol; Hydrogen-Ion Concentration; Isopropyl Thiogalactoside; Lactose; Recombinant Proteins; Whey

2021
IPTG-induced high protein expression for whole-cell biosynthesis of L-phosphinothricin.
    Biotechnology journal, 2023, Volume: 18, Issue:9

    Topics: Escherichia coli; Glutamate Dehydrogenase; Isopropyl Thiogalactoside; Lactose

2023