lactisole and acetosulfame

lactisole has been researched along with acetosulfame* in 5 studies

Other Studies

5 other study(ies) available for lactisole and acetosulfame

ArticleYear
Noncaloric Sweeteners Induce Peripheral Serotonin Secretion via the T1R3-Dependent Pathway in Human Gastric Parietal Tumor Cells (HGT-1).
    Journal of agricultural and food chemistry, 2018, Jul-11, Volume: 66, Issue:27

    The role of sweet taste in energy intake and satiety regulation is still controversial. Noncaloric artificial sweeteners (NCSs) are thought to help reduce energy intake, although little is known about their impact on the satiating neurotransmitter serotonin (5-HT). In the gastrointestinal (GI) tract, 5-HT regulates gastric acid secretion and gastric motility, both part of the complex network of mechanisms regulating food intake and satiety. This study demonstrated a stimulating impact compared to controls (100%) on 5-HT release in human gastric tumor cells (HGT-1) by the NCSs cyclamate (50 mM, 157% ± 6.3%), acesulfame potassium (Ace K, 50 mM, 197% ± 8.6%), saccharin (50 mM, 147% ± 6.7%), sucralose (50 mM, 194% ± 11%), and neohesperidin dihydrochalcone (NHDC, 1 mM, 201% ± 13%). Although these effects were not associated with the sweet taste intensity of the NCSs tested, involvement of the sweet receptor subunit T1R3 in the NCS-evoked response was demonstrated by mRNA expression of TAS1R3, co-incubation experiments using the T1R3 receptor antagonist lactisole, and a TAS1R3 siRNA knockdown approach. Analysis of the downstream signaling revealed activation of the cAMP/ERK/Ca

    Topics: Benzene Derivatives; Cell Line, Tumor; Chalcones; Cyclamates; Cyclic AMP; Gene Expression Regulation; Hesperidin; Humans; Parietal Cells, Gastric; Receptors, G-Protein-Coupled; Saccharin; Serotonin; Signal Transduction; Stomach Neoplasms; Sweetening Agents; Thiazines

2018
Modulation of sweet taste by umami compounds via sweet taste receptor subunit hT1R2.
    PloS one, 2015, Volume: 10, Issue:4

    Although the five basic taste qualities-sweet, sour, bitter, salty and umami-can be recognized by the respective gustatory system, interactions between these taste qualities are often experienced when food is consumed. Specifically, the umami taste has been investigated in terms of whether it enhances or reduces the other taste modalities. These studies, however, are based on individual perception and not on a molecular level. In this study we investigated umami-sweet taste interactions using umami compounds including monosodium glutamate (MSG), 5'-mononucleotides and glutamyl-dipeptides, glutamate-glutamate (Glu-Glu) and glutamate-aspartic acid (Glu-Asp), in human sweet taste receptor hT1R2/hT1R3-expressing cells. The sensitivity of sucrose to hT1R2/hT1R3 was significantly attenuated by MSG and umami active peptides but not by umami active nucleotides. Inhibition of sweet receptor activation by MSG and glutamyl peptides is obvious when sweet receptors are activated by sweeteners that target the extracellular domain (ECD) of T1R2, such as sucrose and acesulfame K, but not by cyclamate, which interact with the T1R3 transmembrane domain (TMD). Application of umami compounds with lactisole, inhibitory drugs that target T1R3, exerted a more severe inhibitory effect. The inhibition was also observed with F778A sweet receptor mutant, which have the defect in function of T1R3 TMD. These results suggest that umami peptides affect sweet taste receptors and this interaction prevents sweet receptor agonists from binding to the T1R2 ECD in an allosteric manner, not to the T1R3. This is the first report to define the interaction between umami and sweet taste receptors.

    Topics: Allosteric Regulation; Benzene Derivatives; Cyclamates; Dipeptides; Drug Interactions; HEK293 Cells; Humans; Protein Binding; Protein Isoforms; Receptors, G-Protein-Coupled; Sodium Glutamate; Sucrose; Sweetening Agents; Taste; Taste Perception; Thiazines

2015
Lactisole inhibits the glucose-sensing receptor T1R3 expressed in mouse pancreatic β-cells.
    The Journal of endocrinology, 2015, Volume: 226, Issue:1

    Glucose activates the glucose-sensing receptor T1R3 and facilitates its own metabolism in pancreatic β-cells. An inhibitor of this receptor would be helpful in elucidating the physiological function of the glucose-sensing receptor. The present study was conducted to examine whether or not lactisole can be used as an inhibitor of the glucose-sensing receptor. In MIN6 cells, in a dose-dependent manner, lactisole inhibited insulin secretion induced by sweeteners, acesulfame-K, sucralose and glycyrrhizin. The IC50 was ∼4 mmol/l. Lactisole attenuated the elevation of cytoplasmic Ca2+ concentration ([Ca2+]c) evoked by sucralose and acesulfame-K but did not affect the elevation of intracellular cAMP concentration ([cAMP]c) induced by these sweeteners. Lactisole also inhibited the action of glucose in MIN6 cells. Thus, lactisole significantly reduced elevations of intracellular [NADH] and intracellular [ATP] induced by glucose, and also inhibited glucose-induced insulin secretion. To further examine the effect of lactisole on T1R3, we prepared HEK293 cells stably expressing mouse T1R3. In these cells, sucralose elevated both [Ca2+]c and [cAMP]c. Lactisole attenuated the sucralose-induced increase in [Ca2+]c but did not affect the elevation of [cAMP]c. Finally, lactisole inhibited insulin secretion induced by a high concentration of glucose in mouse islets. These results indicate that the mouse glucose-sensing receptor was inhibited by lactisole. Lactisole may be useful in assessing the role of the glucose-sensing receptor in mouse pancreatic β-cells.

    Topics: Animals; Benzene Derivatives; Calcium; Cell Line; Cyclic AMP; Glucose; Glycyrrhizic Acid; HEK293 Cells; Humans; Insulin; Insulin Secretion; Insulin-Secreting Cells; Mice; Receptors, G-Protein-Coupled; Sucrose; Sweetening Agents; Thiazines

2015
Diverse signaling systems activated by the sweet taste receptor in human GLP-1-secreting cells.
    Molecular and cellular endocrinology, 2014, Aug-25, Volume: 394, Issue:1-2

    Sweet taste receptor regulates GLP-1 secretion in enteroendocrine L-cells. We investigated the signaling system activated by this receptor using Hutu-80 cells. We stimulated them with sucralose, saccharin, acesulfame K and glycyrrhizin. These sweeteners stimulated GLP-1 secretion, which was attenuated by lactisole. All these sweeteners elevated cytoplasmic cyclic AMP ([cAMP]c) whereas only sucralose and saccharin induced a monophasic increase in cytoplasmic Ca(2+) ([Ca(2+)]c). Removal of extracellular calcium or sodium and addition of a Gq/11 inhibitor greatly reduced the [Ca(2+)]c responses to two sweeteners. In contrast, acesulfame K induced rapid and sustained reduction of [Ca(2+)]c. In addition, glycyrrhizin first reduced [Ca(2+)]c which was followed by an elevation of [Ca(2+)]c. Reductions of [Ca(2+)]c induced by acesulfame K and glycyrrhizin were attenuated by a calmodulin inhibitor or by knockdown of the plasma membrane calcium pump. These results indicate that various sweet molecules act as biased agonists and evoke strikingly different patterns of intracellular signals.

    Topics: Benzene Derivatives; Calcium; Calmodulin; Cell Line, Tumor; Cyclic AMP; Duodenum; Enzyme Inhibitors; Gene Expression Regulation; Glucagon-Like Peptide 1; Glycyrrhizic Acid; Guanine Nucleotide Exchange Factors; Humans; Plasma Membrane Calcium-Transporting ATPases; Receptors, G-Protein-Coupled; RNA, Small Interfering; Saccharin; Signal Transduction; Sucrose; Sweetening Agents; Thiazines

2014
Bitter taste receptors for saccharin and acesulfame K.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 2004, Nov-10, Volume: 24, Issue:45

    Weight-conscious subjects and diabetics use the sulfonyl amide sweeteners saccharin and acesulfame K to reduce their calorie and sugar intake. However, the intrinsic bitter aftertaste, which is caused by unknown mechanisms, limits the use of these sweeteners. Here, we show by functional expression experiments in human embryonic kidney cells that saccharin and acesulfame K activate two members of the human TAS2R family (hTAS2R43 and hTAS2R44) at concentrations known to stimulate bitter taste. These receptors are expressed in tongue taste papillae. Moreover, the sweet inhibitor lactisole did not block the responses of cells transfected with TAS2R43 and TAS2R44, whereas it did block the response of cells expressing the sweet taste receptor heteromer hTAS1R2-hTAS1R3. The two receptors were also activated by nanomolar concentrations of aristolochic acid, a purely bitter-tasting compound. Thus, hTAS2R43 and hTAS2R44 function as cognate bitter taste receptors and do not contribute to the sweet taste of saccharin and acesulfame K. Consistent with the in vitro data, cross-adaptation studies in human subjects also support the existence of common receptors for both sulfonyl amide sweeteners.

    Topics: Aristolochic Acids; Benzene Derivatives; Benzyl Alcohols; Calcium Signaling; Cell Line; Dose-Response Relationship, Drug; Glucosides; GTP-Binding Protein alpha Subunits; Humans; Kidney; Receptors, G-Protein-Coupled; Recombinant Fusion Proteins; Saccharin; Sweetening Agents; Taste; Thiazines; Tongue; Transfection

2004