kt-5720 has been researched along with nefiracetam* in 1 studies
1 other study(ies) available for kt-5720 and nefiracetam
Article | Year |
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Modulation of the neuronal nicotinic acetylcholine receptor-channel by the nootropic drug nefiracetam.
The effects of nefiracetam (DM-9384) on the neuronal nicotinic acetylcholine (ACh) receptor-channel were studied by the whole-cell patch clamp technique using PC12 cells. Nefiracetam had a dual effect on ACh-induced currents: it augmented the currents induced by low concentrations (10-30 microM) of ACh and suppressed those induced by high concentrations (100-1000 microM) of ACh. These effects were reversible after washing with drug-free solution. The stimulating effect of nefiracetam was clearly observed at a concentration of 10 microM, and slight increases in currents were detected even at 0.1 microM or 1 microM. Nefiracetam at 100 microM suppressed the currents induced by a low concentration (10 microM) of ACh. The rate of desensitization of ACh-induced current was greatly accelerated by nefiracetam, and this effect could not be reversed by washing with drug-free solution. When added to the internal pipette solution, the protein kinase A inhibitor KT 5720 (0. 6 microM), but not the protein kinase C inhibitor calphostin C (0.5 microM), abolished the nefiracetam stimulation of the ACh receptor. Pre-incubation of cells with 200 ng/ml pertussis toxin for 24 h also abolished the nefiracetam action. Thus, the nefiracetam modulation of the neuronal nicotinic ACh receptor-channel is exerted via G proteins and protein kinase A. The stimulation of the ACh receptor may be directly related to the cognitive enhancing action of nefiracetam. Topics: Acetylcholine; Animals; Carbazoles; Cyclic AMP-Dependent Protein Kinases; Dose-Response Relationship, Drug; Enzyme Inhibitors; GTP-Binding Proteins; Indoles; Membrane Potentials; Naphthalenes; Neurons; Nootropic Agents; Patch-Clamp Techniques; PC12 Cells; Pertussis Toxin; Protein Kinase C; Pyrroles; Pyrrolidinones; Rats; Receptors, Nicotinic; Signal Transduction; Virulence Factors, Bordetella | 1999 |