krn-7000 has been researched along with isopentenyl-pyrophosphate* in 1 studies
1 other study(ies) available for krn-7000 and isopentenyl-pyrophosphate
Article | Year |
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Activated iNKT cells promote Vγ9Vδ2-T cell anti-tumor effector functions through the production of TNF-α.
Vγ9Vδ2-T cells constitute a proinflammatory lymphocyte subpopulation with established antitumor activity. Phosphoantigens activate Vγ9Vδ2-T cells in vivo and in vitro. We studied whether the antitumor activity of Vγ9Vδ2-T cells can be potentiated by invariant NKT cells (iNKT), an important immunoregulatory T cell subset. When activated by the glycolipid α-galactosylceramide (α-GalCer), iNKT produce large amounts of cytokines involved in antitumor immune responses. Monocyte-derived dendritic cells were loaded with both phosphoantigens (using aminobisphosphonates) and α-GalCer during maturation and subsequently co-cultured with Vγ9Vδ2-T and iNKT cells. Aminobisphosphonates dose-dependently enhanced Vγ9Vδ2-T cell activation, and this was potentiated by α-GalCer-induced iNKT co-activation. iNKT co-activation also enhanced the IFN-γ production and cytolytic potential of Vγ9Vδ2-T cells against tumor cells. Using transwell experiments and neutralizing antibodies cross-talk between iNKT and Vγ9Vδ2-T cells was found to be mediated by TNF-α. Our data provide a rationale for combining both activating ligands to improve Vγ9Vδ2-T cell based approaches in cancer-immunotherapy. Topics: Antigens, Neoplasm; Cells, Cultured; Dendritic Cells; Diphosphonates; Galactosylceramides; Hemiterpenes; Humans; Immunologic Factors; Immunotherapy; Interferon-gamma; Lymphocyte Activation; Natural Killer T-Cells; Neoplasms; Organophosphorus Compounds; T-Lymphocytes, Cytotoxic; Tumor Necrosis Factor-alpha | 2012 |